Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman

Öz

In this study, molecular characterization of endemic Serratula olygocephala grown in Adiyaman was determined. DNA isolation was performed using the Plant DNeasy (Qiagen Inc.) kit, following the kit protocol. In addition, the antioxidant activity and fatty acid composition of the plant were also determined. In terms of the antioxidant activity, total phenolic substance concentration of the plant was determined by applying the folin method and free radical scavenging capacity was determined by DPPH radical scavenging test. Fatty acid profile of Serratula oligocephala was determined by gas chromatography. The percentage of the saturated, monounsaturated, and polyunsaturated fatty acids was calculated.

Anahtar Kelimeler

• : Serratula olygocephala
• moleküler karakterizasyon
• antioksidan
• serbest radikal
• yağ asidi.

Düzeltme: Adıyaman'da Yetişen Endemik Serratula olygocephala’nın Moleküler Karakterizasyonu ve Bazı Biyokimyasal Özelliklerini Belirlenmesi

Öz

Bu çalışmada Adıyaman'da Yetişen Endemik Serratula olygocephala’nın moleküler karakterizasyonu yapıldı. DNA izolasyonu Plant DNeasy (Qiagen Inc.) kiti kullanılarak, kit protokolü takip edilerek yapıldı. Bunun yanında bitkinin antioksidan aktivitesi ve yağ asidi kompozisyonu da belirlenmiştir. Antioksidan etkinliği bakımından, bitkinin toplam fenolik madde konsantrasyonu folin yöntemiyle belirlendi ve serbest radikal süpürme kapasitesini ise invitro olarak DPPH radikal süpürme testi ile tayin edildi. Serratula olygocephala’nın yağ asidi kompozisyonu ise gaz kromatografi yöntemiyle tayin edildi. Doymuş, tekli doymamış ve çoklu doymamış yağ asitleri yüzdesi belirlenmiştir.

Anahtar Kelimeler

• Serratula oligocephala,
• moleküler karakterizasyon
• antioksidan
• serbest radikal
• yağ asidi

Kaynakça

1. Sang, T., Crawford, D.J., & Stuessy, T.F. (1995). Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: Implications for biogeography and concerted evolution. Proceedings of the National Academy of Sciences of the United States of America, 92, 6813-6817. https://doi.org/10.1073/pnas.92.15.6813
2. Taberlet, P., Gielly, L., Pautou, G., & Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17, 1105–1109.
3. Tekeli, Y., Özkaya, A., Tel, A.Z., Deniz Sönmez, G., & Akocak, S. (2019). Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman. Commagene Journal of Biology, 3 (1), 32-36. https://doi.org/10.31594/commagene.789682
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