Comparison of Dye Decolorization Potential of Gluconacetobacter xylinus and Newly Isolated Trametes trogii

Yıl 2025, Cilt: 9 Sayı: 2, 232 - 239, 18.12.2025

Müge Yalçın , Filiz Boran , Özfer Yeşilada

https://doi.org/10.31594/commagene.1681103

Öz

In this study, the dye removal ability of Gluconacetobacter xylinus NRRL B759 during bacterial cellulose production and dye decolorization efficiency of the newly isolated white rot fungus Trametes trogii were investigated. The most efficient results for Reactive Blue 171 dye were obtained on the 6th day under both static and agitated conditions at 30°C. The best results for Remazol Brilliant Blue R dye were determined at 30°C on the 10th and 8th days for static and agitated conditions, respectively. Most importantly, it was observed that biosorption was the main mechanism for dye removal activity of this bacterium. The decolorization ability of fungus was investigated under batch and repeated-batch processes. The most effective decolorization values were 48% on the 8th day for RB171 dye and 83% on the 6th day for RBBR dye under batch process. Decolorization activity of the pellets was tested under repeated-batch experiments in both distilled water and Sabouraud Dextrose Broth (SDB) media. In distilled water media, lower decolorization values were obtained for both dyes compared to SDB media. In SDB medium for RB171, the decolorization values are 70% in the 1st cycle and 19% in the 5th cycle. The RBBR dye decolorization values was 57% in the 1st cycle, while this value was reached 74% in the 5th cycle. The decolorization mechanism of the pellets was mainly a result of microbial metabolism.

Anahtar Kelimeler

Bacterium , bacterial cellulose , dye removal , fungus

Etik Beyan

Ethics committee approval is not required for this study.

Teşekkür

We would like to thank Prof. Dr. Abbas GÜNGÖRDÜ for his assistance with the statistical analysis.

Gluconacetobacter xylinus ve Yeni İzole Edilmiş Trametes trogii'nin Boya Renk Giderimi Potansiyelinin Karşılaştırılması

Öz

Bu çalışmada, Gluconacetobacter xylinus NRRL B759'un bakteriyel selüloz üretimi sırasında boya giderim yeteneği ve yeni izole edilmiş beyaz çürüklük fungus olan Trametes trogii'nin renk giderim etkinliği araştırılmıştır. Reaktif Mavi 171 boyası için en etkili sonuçlar hem statik hem de çalkalamalı koşullarda 30°C'de 6. günde elde edildi. Remazol Parlak Mavi R boyası için ise en iyi sonuçlar, 30°C'deki statik ve çalkalamalı koşullarda sırasıyla 10. gün ve 8. günde belirlendi. En önemlisi, biyosorpsiyonun bu bakterinin boya giderme aktivitesi için ana mekanizma olduğu gözlemlendi. Fungusun renk giderim yeteneği, kesikli ve tekrarlı kesikli süreçler altında araştırılmıştır. Kesikli süreçte en etkili renk giderim değerleri, RB171 boyası için 8. günde %48, RBBR boyası için ise 6. günde %83'tür. Peletlerin renk giderim aktivitesi, hem saf su hem de Sabouraud Dekstroz Broth (SDB) ortamında tekrarlı kesikli süreçte test edilmiştir. Saf su ortamında, her iki boya için de SDB ortamına kıyasla daha düşük renk giderim değerleri elde edilmiştir. SDB ortamında, RB171 için renk giderim değerleri 1. döngüde %70 ve 5. döngüde %19'dur. RBBR boyası renk giderim değerleri 1. döngüde %57 iken, bu değer 5. döngüde %74’e ulaşılmıştır. Peletlerin renk giderim mekanizması esas olarak mikrobiyal metabolizmanın bir sonucudur.

Anahtar Kelimeler

Bakteri , bakteriyel selüloz , boya renk giderimi , fungus

Kaynakça

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