Determination of Fatty Acid Profile and Antioxidant Capacity of Rheum ribes L. Plant Growing in Ovacık District of Tunceli Province

Öz

In this study, the antioxidant capacity, total phenolic, flavonoid, protein, and fatty acid contents of Rheum ribes were evaluated. The plant material was collected from the Ovacık district of Tunceli Province, located in Eastern Anatolia, Türkiye. Methanol extracts were prepared and subjected to various spectrophotometric analyses. Antioxidant activity was assessed using DPPH (2,2-difenil-1-pikrilhidrazil) and ABTS (2,2'-azino-bis(3-etilbenzotiazolin-6-sülfonic acid) radical scavenging assays, total phenolic content was determined by the Folin–Ciocalteu method, flavonoid content by a colorimetric method, total protein content by the Bradford method, and fatty acid content by Gas chromatography (GC). According to the DPPH and ABTS assays, the extracts demonstrated strong radical scavenging activity, with inhibition percentages ranging between 93% and 99%. Total phenolic contents were calculated as 252.10, 267.28 and 267.97 mg GAE/g, while total flavonoid contents were found to be 7.89, 8.14, and 8.23 µg catechin equivalent/g. The average protein content was determined as 68.58 mg/g. The findings reveal that Rheum ribes possesses a high antioxidant potential and that it is rich in phenolic and flavonoid compounds. Additionally, its high protein content suggests that the plant may have structural and metabolic supportive properties beyond its antioxidant effects. These results indicate that it could be a promising candidate for use in the development of functional foods and pharmaceutical formulations.

Anahtar Kelimeler

• Total phenolic content
• flavonoid determination
• protein content
• DPPH free radical scavenging activity
• fatty acids

Tunceli İli Ovacık İlçesinde Yetişen Rheum ribes L. (Işkın) Bitkisinin Yağ Asidi Profili ve Antioksidan Kapasitesinin Belirlenmesi

Öz

Bu çalışmada, Rheum ribes L. (ışkın) bitkisinin antioksidan kapasitesi, toplam fenolik madde, flavonoid ve protein içerikleri değerlendirilmiştir. Bitki materyali, Türkiye'nin Doğu Anadolu Bölgesi’nde yer alan Tunceli ili Ovacık ilçesinden toplanmış ve metanol ekstresi hazırlanarak analizlerde kullanılmıştır. Antioksidan etkinlik DPPH (2,2-difenil-1-pikrilhidrazil) ve ABTS (2,2'-azino-bis(3-etilbenzotiazolin-6-sülfonik asit) serbest radikal süpürme yöntemleriyle belirlenmiş; toplam fenolik içerik Folin–Ciocalteu yöntemiyle, flavonoid içeriği kolorimetrik yöntemle, toplam protein düzeyi ise Bradford yöntemi ile analiz edilmiştir. DPPH ve ABTS yöntemleri sonucunda, ışkın bitkisi ekstresinin serbest radikalleri %93 ile %99 gibi yüksek oranlarda inhibe ettiği gözlemlenmiştir. Toplam fenolik içerik, sırasıyla 252.10; 267.28 ve 267.97 mg GAE/g; toplam flavonoid içerik ise 7.89; 8.14 ve 8.23 µg kateşin eşdeğeri/g olarak hesaplanmıştır. Örneklerin toplam protein düzeyi ise ortalama 68.58 mg/g olarak tespit edilmiştir. Elde edilen bulgular, Rheum ribes bitkisinin güçlü antioksidan kapasiteye sahip olduğunu ve fenolik içerik flavonoid bileşenler bakımından zengin olduğunu ortaya koymuştur. Ayrıca yüksek protein içeriği ile bu bitkinin sadece antioksidan değil, aynı zamanda yapısal ve metabolik açıdan da destekleyici potansiyele sahip olduğu tespit edilmiştir. Bu yönleriyle ışkın bitkisi, fonksiyonel gıda ve farmasötik ürün geliştirme çalışmalarında dikkate değer bir aday olarak değerlendirilmektedir.

Anahtar Kelimeler

• Toplam fenolik içerik
• flavonoid tayini
• protein içeriği
• DPPH serbest radikal süpürme aktivitesi
• yağ asitleri

Kaynakça

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