Gümüş Nanopartiküllerinin Biyosentezi ve Biyosensör Materyali Olarak Kullanımı

Öz

Nanopartiküllerin kullanılabileceği bilim alanlarını arttırmak amacıyla son zamanlarda çeşitli sentezleme metotları geliştirilmeye çalışılmaktadır. Bu metotlardan biri nanopartiküllerin bitkiler aracılığıyla sentezlenmesidir. Günümüzde biyosentez yönteminin kullanılması, fiziksel ve kimyasal yöntemler gibi geleneksel sentez yöntemlerinin sınırlamalarını ortadan kaldırmış, alternatif bir sentez yolu olarak geliştirilmiştir. Bitkisel nanofabrikalar olarak adlandırılan yeşil sentez ile bitkilerde bulunan primer ve sekonder metabolitler nanopartiküllerin indirgenmesi ve kapatıcılığını mümkün kılmaktadır. Bitkilerde bulunan alkaloidler, fenolikler, terpenoidler, ketonlar, polisakkaritler, proteinler, vitaminler, amino asitlerin fonksiyonel grupları iyon halindeki gümüş metalleri ile tepkimeye girerek “+” değerlikli metalleri “0” değerlikli nanometal yapılara indirgemektedir. Aynı zamanda sekonder metabolitlerin fonksiyonel grupları “0” değerlikli gümüş nanopartiküller ile bağlar oluşturarak gümüş nanopartiküllerin yüzeyini kaplar, böylece gümüş nanopartiküllerinin stabilizasyonu sağlanmış olur. Biyolojik yöntemler ile sentez hızlıdır, yüksek verim sağlar ve gümüş nanopartikülü üretimi maliyeti düşer. Aynı zamanda, biyosentez yoluyla nanopartikül üretimi canlı içinde gerçekleştiğinden çevre dostu bir tekniktir. Son teknoloji ile gümüş nanopartiküller, biyosensör ve fotogörüntüleme alanlarında öne çıkmıştır. Gümüş nanopartiküller ile bazı belirteçlerin spesifik olarak tespiti çeşitli çalışmalarla kanıtlanmıştır. Bu derlemede gümüş nanopartiküllerinin kullanım alanları, biyosentezi, stabilizasyonu, karakterizasyonu, antibakteriyel mekanizması ve biyosensör olarak kullanımına değinilecektir.

Anahtar Kelimeler

• Yeşil sentez
• biyonanoteknoloji
• karakterizasyon
• sekonder metabolit
• antibakteriyel aktivite

The Biosynthesis of Silver Nanoparticles and their Use as a Biosensor Material

Öz

Various synthesis methods are being developed in order to increase the number of scientific fields where nanoparticles can be used. Recently, the biosynthesis methods have eliminated the limitations of the traditional synthesis methods such as physical and chemical ones. They have been also developed as an alternative synthesis method. With green synthesis called herbal nanofactories, primary and secondary metabolites in plants enable the reduction and capping of nanoparticles. The functional groups of alkaloids, phenolics, terpenoids, ketones, polysaccharides, proteins, vitamins, and amino acids in plants react with silver metals in ionic form and reduce “+” valued metals to “0” valued nanostructures. At the same time, functional groups of secondary metabolites form bonds with “0” valued silver nanometals and cover the surface of silver nanometals; thus, stabilization is achieved. Synthesis by biological methods provides high efficiency and rapid synthesis and the production cost of silver nanoparticle decreases. Moreover, biosynthesis is an environment-friendly technique as it takes place inside a living being. With the latest technology, silver nanoparticles stand out in the fields of biosensor and photoimaging. In this review, in which areas silver nanoparticles are used and their biosynthesis, stabilization, characterization, antibacterial mechanism, and use as a biosensor will be discussed.

Anahtar Kelimeler

• Green synthesis
• bionanotechnology
• characterization
• secondary metabolite
• antibacterial activity

Kaynakça

1. Abbasi, B.H., Anjum, S., & Hano, C. (2017). Differential effects of in vitro cultures of Linum usitatissimum L. (Flax) on biosynthesis, stability, antibacterial and antileishmanial activities of zinc oxide nanoparticles: a mechanistic approach. Royal Society of Chemistry Advances, 7, 15931-15943. https://doi.org/10.1039/C7RA02070H
2. Abdalla, S.S.I., Katas, H., Chan, J.Y., Ganasan, P., Azmi, F., & Busra, M.F. (2020). Antimicrobial activity of multifaceted lactoferrin or graphene oxide functionalized silver nanocomposites biosynthesized using mushroom waste and chitosan. RSC Advances, 10, 4969-4983. https://doi.org/10.1039/C9RA08680C
3. Ahmed, S.A., Ahmad, M., Swami, B.L., & Ikram, S. (2016). A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced Research, 7 (1), 17-28. https://doi.org/10.1016/j.jare.2015.02.007
4. Alqadi, M.K., Abo Noqtah, O.A., Alzoubi, F.Y., Alzouby, J., & Aljarrah, K. (2014). pH effect on the aggregation of silver nanoparticles synthesized by chemical reduction. Materials Science-Poland, 32, 107-111. https://doi.org/10.2478/s13536-013-0166-9
5. Anjum, S., & Abbasi, B.H. (2016). Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.. International Journal of Nanomedicine, 11, 715-728. https://doi.org/10.2147/IJN.S102359
6. Aref, M.S., & Salem, S.S. (2020). Bio-callus synthesis of silver nanoparticles, characterization, and antibacterial activities via Cinnamomum camphora callus culture. Biocatalysis and Agricultural Biotechnology, 27, 101689. https://doi.org/10.1016/j.bcab.2020.101689
7. Bao, D., Oh, Z.G., & Chen, Z. (2016). Characterization of Silver Nanoparticles Internalized by Arabidopsis Plants Using Single Particle ICP-MS Analysis. Frontiers in Plant Science, 7, 32. https://doi.org/10.3389/fpls.2016.00032
8. Baudot, C., Tan, C.M., & Kong, J.C. (2010). FTIR spectroscopy as a tool for nano-material characterization. Infrared Physics and Technology, 53, 434-438. https://doi.org/10.1016/j.infrared.2010.09.002

9. Calderón-Jiménez, B., Johnson, M.E., Montoro Bustos, A.R., Murphy, K.E., Winchester, M.R., & Vega Baudrit, J.R. (2017). Silver nanoparticles: Technological advances, societal impacts, and metrological challenges. Frontiers in Chemistry, 5(6). https://doi.org/10.3389/fchem.2017.00006
10. Chen, L., Xie, H., & Li, J. (2012). Electrochemical glucose biosensor based on silver nanoparticles/multiwalled carbon nanotubes modified electrode. Journal of Solid State Electrochemistry, 16, 3323-3329. https://doi.org/10.1007/s10008-012-1773-9
11. Chen, S., Quan, Y., Yu, Y.L., & Wang, J.H. (2017). Graphene quantum dot/silver nanoparticle hybrids with oxidase activities for antibacterial application. ACS Biomaterials Science and Engineering, 3, 313-321. https://doi.org/10.1021/acsbiomaterials.6b00644
12. Cheng, G.F., Huang, C.H., Zhao, J., Tan, X.L., Hep, G., & Fang, Y.Z. (2009). A Novel Electrochemical Biosensor for Deoxyribonucleic Acid Detection Based on Magnetite Nanoparticles. Chinese Journal of Analytical Chemistry, 37(2), 169-173. https://doi.org/10.1016/S1872-2040(08)60083-3
13. Choi, O., Yu, C.P., Esteban Fernández, G., & Hu, Z. (2010). Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures. Water Research, 44, 6095-6103. https://doi.org/10.1016/j.watres.2010.06.069
14. Fernando, I., & Zhou, Y. (2019) Impact of pH on the stability, dissolution and aggregation kinetics of silver nanoparticles. Chemosphere, 216, 297-305. https://doi.org/10.1016/j.chemosphere.2018.10.122
15. Gholamreza, A., Varshosaz, J., & Shahbazi, N. (2014). Synthesis of silver nanoparticle using Portulaca oleracea L. extracts. Nanomedicine Journal, 1(2), 94-99.
16. Gonzalez, D.A.C, Leo, B.F., Ruenraroengsak, P., Chen, S., Goode, A.E., Theodorou, İ. G., ………… & Porter, A.E. (2017). Silver nanoparticles reduce brain inflammation and related neurotoxicity through induction of H2S-synthesizing enzymes. Scientific Reports, 7, 42871. https://doi.org/10.1038/srep42871
17. Gorham, J.M., MacCuspie, R.I., Klein, K.L., Fairbrother, D.H., & Holbrook, D. (2012). UV-induced photochemical transformations of citrate-capped silver nanoparticle suspensions. Journal of Nanoparticle Research, 14. https://doi.org/10.1007/s11051-012-1139-3
18. Gudikandula, K., & Maringanti, S.C. (2016). Synthesis of silver nanoparticles by chemical and biological methods and their antimicrobial properties. Journal of Experimental Nanoscience, 11(9). https://doi.org/10.1080/17458080.2016.1139196
19. Hegazy, H.G., Rabie, G.H., Shabaan, L.D., & Raie, D.S. (2014). Extracellular Synthesis of Silver Nanoparticles by Callus of Medicago sativa. Life Science Journal, 11(10), 1211-1214.
20. Hegazy, H.G., Shabaan, L.D., Rabie, G.H., & Raie, D.S. (2015). Biosynthesis of Silver Nanoparticles Using Cell Free Callus Exudates of Medicago sativa L. Pakistan Journal of Botany, 47(5), 1825-1829.
21. Howe, P.D., & Dobson, S. (2002). Silver and Silver Compounds: Environmental Aspects, World Health Organization & International Programme on Chemical Safety, Geneva.
22. Iravani, S., Korbekandi, H., Mirmohammadi, S.V., & Zolfaghari, B. (2014). Synthesis of silver nanoparticles: chemical, physical and biological methods. Research in Pharmaceutical Sciences, 9(6), 385-406.
23. Javed, R., Zia, M., Naz, S, Aisida, S.O., Ain, N., & Ao, Q. (2020). Role of capping agents in the application of nanoparticles in biomedicine and environmental remediation: recent trends and future prospects. Journal of Nanobiotechnology, 18, 172. https://doi.org/10.1186/s12951-020-00704-4
24. Khatami, M., Nejad, M.S., Salari, S., & Almani, P.G.N. (2015). Plant-mediated green synthesis of silver nanoparticles using Trifolium resupinatum seed exudate and their antifungal efficacy on Neofusicoccum parvum and Rhizoctonia solani. IET Nanobiotechnology, 10, 4, 237-243. https://doi.org/10.1049/iet-nbt.2015.0078
25. Kim, J.Y., & Lee, J.S. 2012. Multiplexed DNA Detection with DNA-Functionalized Silver and Silver/Gold Nanoparticle Superstructure Probes. Bulletin of the Korean Chemical Society, 33(1), 221. http://dx.doi.org/10.5012/bkcs.2012.33.1.221
26. Kumar, K.S., & Kathireswari, P. (2016). Biological synthesis of Silver nanoparticles (Ag-NPS) by Lawsonia inermis (Henna) plant aqueous extract and its antimicrobial activity against human pathogens. International Journal of Current Microbiology and Applied Sciences, 5(3), 926-937. http://dx.doi.org/10.20546/ijcmas.2016.503.107
27. Kvitek, L., Panáček, A., Soukupová, J., Kolář, M., Večeřová, R., Prucek, R., Holecová, M., & Zbořil, R. (2008). Effect of Surfactants and Polymers on Stability and Antibacterial Activity of Silver Nanoparticles (NPs). The Journal of Physical Chemistry C, 112(15), 5825-5834. https://doi.org/10.1021/jp711616v
28. Liu, X., Knauer, M., Ivleva, N.P., Niessner, R., & Haisch, C. (2010). Synthesis of Core−Shell Surface-Enhanced Raman Tags for Bioimaging. Analytical Chemistry, 82(1), 441-446. https://doi.org/10.1021/ac902573p
29. Loiseau, A., Asila, V., Boitel-Aullen, G., Lam, M., Salmain, M., & Boujday, S. (2019). Silver-Based Plasmonic Nanoparticles for and Their Use in Biosensing. Biosensors, 9(2), 78. https://doi.org/10.3390/bios9020078
30. Lukman, A.I., Gong, B., Marjo, C.E., Roessner, U., & Harris, U.T. (2011). Facile synthesis, stabilization, and anti-bacterial performance of discrete Ag nanoparticles using Medicago sativa seed exudates. Journal of Colloid and Interface Science, 353, 433-444. https://doi.org/10.1016/j.jcis.2010.09.088
31. Mandeh, M., Omidi, M., & Rahaie, M. (2012). In Vitro Influences of TiO2 Nanoparticles on Barley (Hordeum vulgare L.). Tissue Culture, Biological Trace Element Research, 150, 376-380. https://doi.org/10.1007/s12011-012-9480-z
32. Moldovan, B., David, L., Achim, M., Clichici, S., & Filip, G.A. (2016). A green approach to phytomediated synthesis of silver nanoparticles using Sambucus nigra L. fruits extract and their antioxidant activity. Journal of Molecular Liquids, 221, 271-278. https://doi.org/10.1016/j.molliq.2016.06.003
33. Mude, N., Ingle, A., Gade, A., & Rai, M. (2009). Synthesis of Silver Nanoparticles Using Callus Extract of Carica papaya – A First Report. Journal of Plant Biochemistry and Biotechnology, 18(1), 83-86. https://doi.org/10.1007/BF03263300
34. Mukherjee, S., Chowdhury D., Kotcherlakota R., Patra S., Vinothkumar B., Bhadra M.P., …… & Patra, C.R. (2014). Potential theranostics application of bio-synthesised silver nanoparticles (4-in-1 system). Theranostics, 4, 316-335. https://doi.org/10.7150/thno.7819
35. Nabikhan, A., Kandasamy, K., Raj, A., & Alikunhi, N.M. (2010). Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L.. Colloids and Surfaces B: Biointerfaces, 79, 488-493. https://doi.org/10.1016/j.colsurfb.2010.05.018
36. Naja, G., Bouvrette, P., Hrapovic, S., & Luong, J.H.T. (2007). Raman-based detection of bacteria using silver nanoparticles conjugated with antibodies. Analyst, 132(7), 679-86. https://doi.org/10.1039/B701160A
37. Netala, V.R., Kotakadi, V.S., Nagam, V., Bobbu, P., Ghosh, S.B., & Tartte, V. (2015). First report of biomimetic synthesis of silver nanoparticles using aqueous callus extract of Centella asiatica and their antimicrobial activity. Applied Nanoscience, 5, 801-807. https://doi.org/10.1007/s13204-014-0374-6
38. Oliveira, P. F. M., Michalchuk, A. A. L., Marquardt, J., Feiler, T., Prinz, C., Torresi, R. M., & Emmerling, F. (2020). Investigating the Role of Reducing Agents on Mechanosynthesis of Au Nanoparticles. CrystEngComm, 22, 6261-6267. https://doi.org/10.1039/D0CE00826E
39. Pal, S., Nisi, R., Stoppa, M., & Licciulli, A. (2017). Silver-Functionalized bacterial cellulose as antibacterial membrane for wound-healing applications. ACS Omega, 2, 3632-3639. https://doi.org/10.1021/acsomega.7b00442
40. Parvathiraja, C., Shailajha, S., Shanavas, S., & Gurung J. (2021). Biosynthesis of silver nanoparticles by Cyperus pangorei and its potential in structural, optical and catalytic dye degradation. Applied Nanoscience, 11, 477-491. https://doi.org/10.1007/s13204-020-01585-7
41. Patra, J.K., Kwon, Y., & Baek, K.H. (2016). Green biosynthesis of gold nanoparticles by onion peel extract: Synthesis, characterization and biological activities. Advanced Powder Technology, 27, 2204-2213. https://doi.org/10.1016/j.apt.2016.08.005
42. Ramanathan, S., Gopinath, S.C.B., Anbu, P., Lakshmipriya, T., Kasim, F.H., & Lee, C.G. (2018). Eco-friendly synthesis of Solanum trilobatum extract-capped silver nanoparticles is compatible with good antimicrobial activities. Journal of Molecular Structure, 1160, 80-91. https://doi.org/10.1016/j.molstruc.2018.01.056
43. Rasheed, T., Bilal, M., Iqbal, H.M.N., & Li, C. (2017). Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications. Colloids and Surfaces B: Biointerfaces, 158, 408-15. https://doi.org/10.1016/j.colsurfb.2017.07.020
44. Rashid, S., Azeem, M., Khan, S.A., Shah, M.M., & Ahmad, R. (2019). Characterization and synergistic antibacterial potential of green synthesized silver nanoparticles using aqueous root extracts of important medicinal plants of Pakistan. Colloids and Surfaces B: Biointerfaces, 179, 317-25. https://doi.org/10.1016/j.colsurfb.2019.04.016
45. Rashmi, V., Prabhushankar, H.B., & Sanjay, K.R. (2021). Centella asiatica L. callus mediated biosynthesis of silver nanoparticles, optimization using central composite design, and study on their antioxidant activity. Plant Cell Tissue Organ Culture, 146, 515-529. https://doi.org/10.1007/s11240-021-02086-3
46. Sallah, A., Naomi, R., Utami, N.D., Mohammad, A.W., Mahmoudi, E., Mustafa, N., & Fauzi, B. (2020). The Potential of Silver Nanoparticles for Antiviral and Antibacterial Applications: A Mechanism of Action. Nanomaterials, 10(8), 1566. https://doi.org/10.3390/nano10081566
47. Satyavani, K., Ramanathan, Y., & Gurudeeban, S. (2011). Green Synthesis of Silver Nanoparticles by Using Stem Derived Callus Extract of Bitter Apple (Citrullus Colocynthis). Digest Journal of Nanomaterials and Biostructures, 6(3), 1019-1024. https://doi.org/10.1007/1019_Satyavani.pdf
48. Sharifi-Rad, M., Pohl, P., Epifano, F., & Álvarez-Suarez, J.M. (2020). Green Synthesis of Silver Nanoparticles Using Astragalus tribuloides Delile. Root Extract: Characterization, Antioxidant, Antibacterial, and Anti-Inflammatory Activities, Nanomaterials, 10, 2383. https://doi.org/10.3390/nano10122383
49. Siddiqi, K.S., & Husen, A. (2017). Recent advances in plant-mediated engineered gold nanoparticles and their application in biological system. Journal of Trace Elements in Medicine and Biology, 40, 10-23. https://doi.org/10.1016/j.jtemb.2016.11.012
50. Singh, P., Kim, Y.J., Zhang, D., & Yang, D.C. (2016). Biological Synthesis of Nanoparticles from Plants and Microorganisms. Trends in Biotechnology, 34, 7. https://doi.org/10.1016/j.tibtech.2016.02.006
51. Song, J., Wu, F., Wan, Y., & Ma, L. (2015). Colorimetric detection of melamine in pretreated milk using silver nanoparticles functionalized with sulfanilic acid. Food Control, 50, 356-361. https://doi.org/10.1016/j.foodcont.2014.08.049
52. Sotiriou, G.A., & Pratsinis, S.E. (2011). Engineering nanosilver as an antibacterial, biosensor and bioimaging material. Current Opinion in Chemical Engineering, 1(1), 3-10. https://doi.org/10.1016/j.coche.2011.07.001
53. Szymanski, M.S., & Porter, R.A. (2013). Preparation and quality control of silver nanoparticle–antibody conjugate for use in electrochemical immunoassays. Journal of Immunological Methods, 387(1-2), 262-269. https://doi.org/10.1016/j.jim.2012.11.003
54. Tan, P., Li, H.S., Wang, J., & Gopinath, S.C.B. (2020). Silver nanoparticle in biosensor and bioimaging: Clinical perspectives. Biotechnology and Applied Biochemistry, https://doi.org/10.1002/bab.2045
55. Tian, X., Jiang, X., Welch, C., Croley, T.R., Wong, T.Y., Chen, C., ……. & Yin, J.J. (2018). Bactericidal Effects of Silver Nanoparticles on Lactobacilli and the Underlying Mechanism. ACS Applied Materials & Interfaces, 10, 8443-8450. https://doi.org/10.1021/acsami.7b17274
56. Toh, S., Jurkschat, K., & Compton, R.G. (2015). The Influence of the Capping Agent on the Oxidation of Silver Nanoparticles: Nano-impacts versus Stripping Voltammetry. Chemistry-A European Journal, 21, 2998-3004. https://doi.org/10.1002/chem.201406278
57. Vanaja, M., & Annadurai, G. (2013). Coleus aromaticus leaf extract mediated synthesis of silver nanoparticles and its bactericidal activity. Applied Nanoscience, 3, 217-223. https://doi.org/10.1007/s13204-012-0121-9
58. Vennila, K., Chitra, L., Balagurunathan, R., & Palvannan, T. (2018). Comparison of biological activities of selenium and silver nanoparticles attached with bioactive phytoconstituents: green synthesized using Spermacoce hispida extract. Advances in Natural Sciences: Nanoscience and Nanotechnology, 9, 5-15. https://doi.org/10.1088/2043-6254/aa9f4d
59. Wakshlak, R.B.K., Pedahzur, R., & Avnir, D. (2015). Antibacterial activity of silver-killed bacteria: The “zombies” effect. Scientific Reports, 5, 1-5. https://doi.org/10.1038/srep09555
60. Xia, Q.H., Ma, Y.J., & Wang, J.W. (2016). Biosynthesis of Silver Nanoparticles Using Taxus yunnanensis Callus and Their Antibacterial Activity and Cytotoxicity in Human Cancer Cells. Nanomaterials, 6, 160. https://doi.org/10.3390/nano6090160
61. Xuan, Z., Li, M., Rong, P., Wang, W., Li, Y., & Liu, D. (2016). Plasmonic ELISA based on the controlled growth of silver nanoparticles. Nanoscale, 39. https://doi.org/10.1039/C6NR06079J
62. Zhang, H., & Zhang, C. (2014). Transport of silver nanoparticles capped with different stabilizers in water saturated porous media. Journal of Materials and Environmental Science, 5:231-236.
63. Zhao, L., Yu, R.J., Ma, W., Han, H.X., Tian, H., Qian, R.C., & Long, Y.T. (2017). Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay. Theranostics, 7(4), 876-883. https://www.thno.org/v07p0876.htm
64. Zook, J.M., Long, S.E., Cleveland, D., Geronimo, C.L.A., & MacCuspie, R.I. (2011). Measuring silver nanoparticle dissolution in complex biological and environmental matrices using UV–visible absorbance. Analytical and Bioanalytical Chemistry, 401, 1993. https://doi.org/10.1007/s00216-011-5266-y