Araştırma Makalesi
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Morus spp. Genotiplerinin Klonal Anaç Potansiyeli ve Morus nigra L. ile Aşı Uyumu Üzerine Biyokimyasal Etkileri

Yıl 2025, Cilt: 13 Sayı: 2, 352 - 362, 24.12.2025
https://doi.org/10.33202/comuagri.1819775

Öz

Meyve ağaçlarında uygun anaç seçimi, hem verimlilik hem de aşı başarısı açısından büyük önem taşımaktadır. Karadut (Morus nigra L.) fidanı üretiminde yaygın olarak aşılama kullanılmakta olup, aşılamayı izleyen dönemde veya bitki gelişiminin ilerleyen safhalarında anaç–kalem uyuşmazlığı ortaya çıkabilmektedir. Bu araştırmada 56 farklı Morus spp. genotipi çelikle çoğaltıldı ve köklenme oranı %70’in üzerinde olan 21 genotip Ekşi Kara çeşidi ile aşılandı. Biyokimyasal analizler sonucunda, aşı başarısı yüksek genotiplerde toplam şeker, nişasta ve toplam karbonhidrat içeriklerinin daha yüksek olduğu saptandı. Ayrıca, peroksidaz ve askorbat peroksidaz enzim aktiviteleri ile toplam fenolik bileşik düzeylerinde artış gözlendi. He Ya Bai genotipi yüksek karbonhidrat ve toplam fenolik bileşik değerleriyle dikkat çekerken, Yediveren 24-08 ve Ayaş Beyazı genotiplerinde yüksek peroksidaz ve askorbat peroksidaz aktiviteleri belirlendi. Bulgular, aşı başarısının sadece anatomik uyuma bağlı olmadığını, aynı zamanda anaç ve kalemdeki fizyolojik ve biyokimyasal durumun da belirleyici olduğunu göstermektedir. Bu çalışma, M. nigra’ya uygun klonal anaçların belirlenmesine yönelik bilimsel bir temel sunmakta ve aşı başarısını artırmaya yönelik stratejik öneriler sağlamaktadır.

Kaynakça

  • Alagöz, H.Ş., 2022. Farklı zamanlarda alınan ceviz aşı kalemlerinde biyokimyasal değişikliklerin ve aşı başarılarına olan etkilerinin incelenmesi. Aydın Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Yüksek Lisans Tezi, Aydın, 68 s.
  • Anis, M., Faisal, M., Singh, S.K., 2003. Micropropagation of mulberry (Morus alba L.) through in vitro culture of shoot tip and nodal explants. Plant Tissue Culture. 13 (1): 47–51.
  • Aziz, R.R., Mohammed, A.A., Ahmad, F.K., Ali, A.J., 2024. Effect of IBA concentration and water soaking on rooting hardwood cuttings of black mulberry (Morus nigra L.). arXiv preprint, arXiv:2408.11083.
  • Bharathi, K.M., Susikaran, S., Parthiban, K.T., Vasanth, V., Harika, R.K., Garai, I., Mithilasri, M., Sabarish, M., Kalpana, R., Navaneethan, S., Niranjan, M.A., Vijay, 2024. A study to evaluate the effect of using different concentrations of rooting hormone on root and shoot characters of apical cuttings of V1 mulberry (Morus indica L.) raised using mini clonal technology at nursery level. International Journal of Research in Agronomy. SP-7 (8): 38–42
  • Borsai, O., Cosmin, D., Lukács, L., Andrecan, F., Mitre, V., Negrușier, C., Rózsa, S., 2024. The influence of substrate and cuttings’ type on rooting capacity of Morus alba species. Journal of Horticulture, Forestry and Biotechnology. 28 (2): 52–59.
  • Boudet, A.M., Kajita, S., Grima-Pettenati, J., Goffner, D., 2003. Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends in Plant Science. 8: 576–581.
  • Çakmak, I., 1994. Activity of ascorbate-dependent H₂O₂-scavenging enzymes and leaf chlorosis are enhanced in magnesium- and potassium-deficient leaves, but not in phosphorus-deficient leaves. Journal of Experimental Botany. 45 (9): 1259–1266.
  • Can, A., Kazankaya, A., Orman, E., Gündoğdu, M., Ercişli, S., Choudhary, R., Karunakaran, R., 2021. Sustainable mulberry (Morus nigra L., Morus alba L., and Morus rubra L.) production in eastern Turkey. Sustainability. 13 (24): 13507.
  • Çekiç, Ç., Erdem, S.Ö., Aydemir, M., 2013. The effects of paclobutrazol and IBA treatments on the rooting of wood-cuttings of black mulberry (Morus nigra L.) and red mulberry (Morus rubra L.). Research Journal of Agricultural Sciences. 1: 174–177.
  • Çezik, F., Çekiç, Ç., Yıldız, K., Karadağ, H., Öcalan, O.N., 2022. Effects of light and dark conditions on root development of black mulberry (Morus nigra L.) hardwood cuttings. Turkish Journal of Agriculture: Food Science and Technology. 10 (sp1): 2718–2721.
  • Chandler, S.F., Dodds, J.H., 1983. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum. Plant Cell Reports. 2 (4): 105–108.
  • Cui, Q., Xie, L., Dong, C., Gao, L., Shang, Q., 2021. Stage-specific events in tomato graft formation and the regulatory effects of auxin and cytokinin. Plant Science. 304: 110803.
  • Dinçer, E., Saraçoğlu, O., Öcalan, O.N., 2022. Rooting performances of promising black mulberry (Morus nigra L.) genotypes determined in Tokat Province under same environmental conditions. Turkish Journal of Agriculture-Food Science and Technology. 10: 2708–2712.
  • Erdoğan, V., Aygün, A., 2006. Karadut’un (Morus nigra L.) yeşil çelikle çoğaltılması üzerinde bir araştırma. II. Ulusal Üzümsü Meyveler Sempozyumu, 14–16 Eylül, Tokat, ss. 172–175.
  • Foyer, C.H., Kunert, K., 2024. The ascorbate–glutathione cycle: coming of age. Journal of Experimental Botany. 75: 2682–2699.
  • Hartmann, H.T., Kester, D.E., Geneve, R.L., 2011. Hartmann and Kester’s Plant Propagation: Principles and Practices. 8. baskı. Prentice Hall, Upper Saddle River, New Jersey, ABD, 915 s.
  • Irisarri, P., Binczycki, P., Errea, P., Martens, H.J., Pina, A., 2015. Oxidative stress associated with rootstock-scion interactions in pear/quince combinations during early stages of graft development. Journal of Plant Physiology. 176: 25–35.
  • İşbilir, M.E., Saraçoğlu, O., Dinçer, E., Donat, A., Al-Salihi, A.A.M., 2022. Effects of paclobutrazol applications on rooting performance of black mulberry (Morus nigra L.) hardwood cuttings. Turkish Journal of Agriculture-Food Science and Technology. 10: 2722–2724.
  • Karimi, H.R., Nowrozy, M., 2017. Effects of rootstock and scion on graft success and vegetative parameters of pomegranate. Scientia Horticulturae. 214: 280–287.
  • Kawaguchi, M., Taji, A., Backhouse, D., Oda, M., 2008. Anatomy and physiology of graft incompatibility in solanaceous plants. Journal of Horticultural Science & Biotechnology. 83: 581–588.
  • Koç, M., 2011. Farklı köklenme ortam sıcaklığı ve nem değerinin karadut odun çeliklerinin köklenmesi üzerine etkileri. Gaziosmanpaşa Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 46 s.
  • Koyuncu, F., Şenel, E., 2003. Rooting of black mulberry (Morus nigra L.) hardwood cuttings. Journal of Fruit and Ornamental Plant Research. 11: 53–57.
  • Kurotani, K.I., Notaguchi, M., 2021. Cell-to-cell connection in plant grafting—molecular insights into symplastic reconstruction. Plant Cell Physiology. 62 (9): 1362–1371.
  • Lal, N., Ramteke, V., Diwan, G., et al., 2025. Physiological, biochemical, and molecular responses during grafting in horticultural crops. Plant Molecular Biology Reporter. 1–22.
  • Li, P., Sayre, K.D., 1975. The protein, non-protein, and total nitrogen in Solanum tuberosum spp. andigena potatoes. American Potato Journal. 52: 341–352.
  • Loupit, G., Cookson, S.J., 2020. Identifying molecular markers of successful graft union formation and compatibility. Frontiers in Plant Science. 11: 1903.
  • Luo, J., Wang, Y., Zhao, A.Z., 2019. The complete chloroplast genome of Morus alba (Moraceae: Morus), the herbal medicine species in China. Mitochondrial DNA Part B. 4 (2): 2467–2468.
  • MacAdam, J.W., Nelson, C.J., Sharp, R.E., 1992. Peroxidase activity in the leaf elongation zone of tall fescue: I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiology. 99 (3): 872–878.
  • Miao, L., Li, S.Z., Bai, L.Q., Anwar, A., Li, Y.S., He, C.X., Yu, X.C., 2019. Effect of grafting methods on physiological change of graft union formation in cucumber grafted onto bottle gourd rootstock. Scientia Horticulturae. 244: 249–256.
  • Mosse, B., 1962. Graft incompatibility in fruit trees. Technical communication no. 28, Comm. Bur. Horticulture and Plant Crops, East Malling.
  • Musacchi, S., Masia, A., Fachinello, J., 2002. Variation of some enzymatic activities in relationship to scion/stock compatibility in pear/quince combinations. Acta Horticulturae. 596: 389–392.
  • Nakano, Y., Asada, K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology. 22 (5): 867–880.
  • Nanda, A.K., Melnyk, C.W., 2018. The role of plant hormones during grafting. Journal of Plant Research. 131 (1): 49–58.
  • Narayan, P.S., Chakraborty, G., Subba, R., 1989. Regulation of plantlets from the callus of stem segment of mature plant of Morus alba L. Proceedings of the Indian National Science Academy. 55: 469–472.
  • Nicholson, R.L., Hammerschmidt, R., 1992. Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology. 30: 369–389.
  • Özgür, M., Uçar, A., Yılmaz, S., 2025. The multifaceted benefits of Morus nigra L.: a pharmacological powerhouse. Phytochemistry Reviews. 24: 5317–5342.
  • Peng, C., Gao, F., Wang, H., Shen, H., Yang, L., 2020. Physiological and biochemical traits in Korean pine somatic embryogenesis. Forests. 11 (5): 577.
  • Peters, J.L., Castillo, F.J., Heath, R.L., 1989. Alteration of extracellular enzymes in pinto bean leaves upon exposure to air pollutants, ozone, and sulfur dioxide. Plant Physiology. 89 (1): 159–164.
  • Phillips, N., Reynolds, A., Di Profio, F., 2015. Nonstructural carbohydrate concentrations in dormant grapevine scionwood and rootstock impact propagation success and vine growth. HortTechnology. 25 (4): 536–550.
  • Quessada, M.P., 1984. Les Peroxidases de l'abricotier (Prunus armeniaca L.) et Leur Intervention Dans l'incompatibilité au Greffage. These 3ème cycle, Montpellier.
  • Regmi, S., Puri, C., Shah, K., 2022. IBA impact at different dose on mulberry cutting (Morus alba). Research in Agriculture, Livestock and Fisheries. 9 (3): 253–257.
  • Reig, G., Font, I., Forcada, C., Mestre, L., Jimenez, S., Betrán, J.A., Moreno, M.A., 2018. Horticultural leaf mineral and fruit quality traits of two ‘greengage’ plum cultivars budded on plum based rootstocks in Mediterranean conditions. Scientia Horticulturae. 232: 84–91.
  • Sivaci, A., 2006. Seasonal changes of total carbohydrate content in three varieties of apple (Malus sylvestris Miller) stem cuttings. Scientia Horticulturae. 109: 234–237.
  • Slinkard, S., Singleton, V.L., 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 28 (1): 49–55.
  • Su, W.C., He, H.Y., Liu, Z.Z., Mo, Z.H., Cao, F., Peng, F.R., 2021. Physiological and biochemical changes during graft union formation in Carya illinoinensis. Biologia Plantarum. 65 (2): 203–211.
  • Telles, C.A., Biasi, L.A., Mindêllo Neto, U.R., Deschamps, C., 2009. Fenóis totais, peroxidase e suas relações com a compatibilidade de mudas de pessegueiro interenxertadas. Ciência e Agrotecnologia. 33 (1): 86–91.
  • Uğur, R., 2025. Evaluation of suitable rootstock and key criteria for rootstock breeding in Prunus species. Applied Fruit Science. 67 (6): 475.
  • Uğur, R., Paydaş, S., Saridas, M.A., 2023. Rootstock breeding and rootstock-scion interaction in Prunus species. Erciyes Tarım ve Hayvan Bilimleri Dergisi. 6 (2): 7–10.
  • Ünal, A., Özçağıran, R., Hepaksoy, S., 1992. Kara dut ve mor dut çeşitlerinde odun çeliklerinin köklenmesi üzerinde bir araştırma. Türkiye I. Ulusal Bahçe Bitkileri Kongresi, 1: 267–270.
  • Vural, U., Dumanoğlu, H., Erdoğan, V., 2008. Effect of grafting/budding techniques and time on propagation of black mulberry (Morus nigra L.) in cold temperate zones. Propagation of Ornamental Plants. 8 (2): 55–58.
  • Yıldız, K., Çekiç, Ç., Güneş, M., Özgen, M., Özkan, Y., Akça, Y., Gerçekçioğlu, R., 2009. Farklı dönemlerde alınan karadut (Morus nigra L.) çelik tiplerinde köklenme başarısının belirlenmesi. GOÜ. Zir. Fak. Der. 26: 1–5.
  • Yılmaz, M., 1992. Bahçe Bitkileri Yetiştirme Tekniği. Ç.Ü. Basımevi, Adana.
  • Zarrouk, O., Testillano, P.S., Risueno, M.C., Moreno, M.A., Gogorcena, Y., 2010. Changes in cell/tissue organization and peroxidase activity as markers for early detection of graft incompatibility in peach/plum combinations. Journal of the American Society for Horticultural Science. 135: 9–17.
  • Zhang, J., Liu, Y., Li, C., Yin, B., Liu, X., Guo, X., Zhang, C., Liu, D., Hwang, I., Li, H., et al., 2022. PtomtAPX is an autonomous lignification peroxidase during the earliest stage of secondary wall formation in Populus tomentosa Carr. Nature Plants. 8 (7): 828–839.

Clonal Rootstock Potential of Morus spp. Genotypes and Their Biochemical Influence on Graft Compatibility in Morus nigra L.

Yıl 2025, Cilt: 13 Sayı: 2, 352 - 362, 24.12.2025
https://doi.org/10.33202/comuagri.1819775

Öz

Selecting an appropriate rootstock in fruit trees is crucial for both productivity and grafting success. In black mulberry (Morus nigra L.) propagation, grafting is widely used; however, graft incompatibility may occur following grafting or during later stages of plant development. In this study, 56 different Morus spp. genotypes were propagated via cuttings, and 21 genotypes with rooting success above 70% were grafted onto the Ekşi Kara cultivar. Biochemical analyses revealed that genotypes with high grafting success had higher total sugars, starch, and total carbohydrates. In addition, increased peroxidase and ascorbate peroxidase enzyme activities, as well as higher total phenolic compound levels, were observed. The He Ya Bai genotype stood out for its high total carbohydrates and total phenolic compound levels, while Yediveren 24-08 and Ayaş Beyazı genotypes showed elevated peroxidase and ascorbate peroxidase activities. These findings indicate that grafting success is not solely determined by anatomical compatibility, but is also influenced by the physiological and biochemical status of both rootstock and scion. This study provides a scientific basis for identifying suitable clonal rootstocks for M. nigra and offers strategic guidance to enhance grafting success.

Teşekkür

I would like to express my sincere gratitude to Associate Prof. Dr. Feyza İnceoğlu for her valuable assistance with statistical analysis throughout this study. I also thank the Malatya Apricot Research Institute for providing the necessary materials and laboratory facilities for the research.

Kaynakça

  • Alagöz, H.Ş., 2022. Farklı zamanlarda alınan ceviz aşı kalemlerinde biyokimyasal değişikliklerin ve aşı başarılarına olan etkilerinin incelenmesi. Aydın Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilim Dalı, Yüksek Lisans Tezi, Aydın, 68 s.
  • Anis, M., Faisal, M., Singh, S.K., 2003. Micropropagation of mulberry (Morus alba L.) through in vitro culture of shoot tip and nodal explants. Plant Tissue Culture. 13 (1): 47–51.
  • Aziz, R.R., Mohammed, A.A., Ahmad, F.K., Ali, A.J., 2024. Effect of IBA concentration and water soaking on rooting hardwood cuttings of black mulberry (Morus nigra L.). arXiv preprint, arXiv:2408.11083.
  • Bharathi, K.M., Susikaran, S., Parthiban, K.T., Vasanth, V., Harika, R.K., Garai, I., Mithilasri, M., Sabarish, M., Kalpana, R., Navaneethan, S., Niranjan, M.A., Vijay, 2024. A study to evaluate the effect of using different concentrations of rooting hormone on root and shoot characters of apical cuttings of V1 mulberry (Morus indica L.) raised using mini clonal technology at nursery level. International Journal of Research in Agronomy. SP-7 (8): 38–42
  • Borsai, O., Cosmin, D., Lukács, L., Andrecan, F., Mitre, V., Negrușier, C., Rózsa, S., 2024. The influence of substrate and cuttings’ type on rooting capacity of Morus alba species. Journal of Horticulture, Forestry and Biotechnology. 28 (2): 52–59.
  • Boudet, A.M., Kajita, S., Grima-Pettenati, J., Goffner, D., 2003. Lignins and lignocellulosics: a better control of synthesis for new and improved uses. Trends in Plant Science. 8: 576–581.
  • Çakmak, I., 1994. Activity of ascorbate-dependent H₂O₂-scavenging enzymes and leaf chlorosis are enhanced in magnesium- and potassium-deficient leaves, but not in phosphorus-deficient leaves. Journal of Experimental Botany. 45 (9): 1259–1266.
  • Can, A., Kazankaya, A., Orman, E., Gündoğdu, M., Ercişli, S., Choudhary, R., Karunakaran, R., 2021. Sustainable mulberry (Morus nigra L., Morus alba L., and Morus rubra L.) production in eastern Turkey. Sustainability. 13 (24): 13507.
  • Çekiç, Ç., Erdem, S.Ö., Aydemir, M., 2013. The effects of paclobutrazol and IBA treatments on the rooting of wood-cuttings of black mulberry (Morus nigra L.) and red mulberry (Morus rubra L.). Research Journal of Agricultural Sciences. 1: 174–177.
  • Çezik, F., Çekiç, Ç., Yıldız, K., Karadağ, H., Öcalan, O.N., 2022. Effects of light and dark conditions on root development of black mulberry (Morus nigra L.) hardwood cuttings. Turkish Journal of Agriculture: Food Science and Technology. 10 (sp1): 2718–2721.
  • Chandler, S.F., Dodds, J.H., 1983. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum. Plant Cell Reports. 2 (4): 105–108.
  • Cui, Q., Xie, L., Dong, C., Gao, L., Shang, Q., 2021. Stage-specific events in tomato graft formation and the regulatory effects of auxin and cytokinin. Plant Science. 304: 110803.
  • Dinçer, E., Saraçoğlu, O., Öcalan, O.N., 2022. Rooting performances of promising black mulberry (Morus nigra L.) genotypes determined in Tokat Province under same environmental conditions. Turkish Journal of Agriculture-Food Science and Technology. 10: 2708–2712.
  • Erdoğan, V., Aygün, A., 2006. Karadut’un (Morus nigra L.) yeşil çelikle çoğaltılması üzerinde bir araştırma. II. Ulusal Üzümsü Meyveler Sempozyumu, 14–16 Eylül, Tokat, ss. 172–175.
  • Foyer, C.H., Kunert, K., 2024. The ascorbate–glutathione cycle: coming of age. Journal of Experimental Botany. 75: 2682–2699.
  • Hartmann, H.T., Kester, D.E., Geneve, R.L., 2011. Hartmann and Kester’s Plant Propagation: Principles and Practices. 8. baskı. Prentice Hall, Upper Saddle River, New Jersey, ABD, 915 s.
  • Irisarri, P., Binczycki, P., Errea, P., Martens, H.J., Pina, A., 2015. Oxidative stress associated with rootstock-scion interactions in pear/quince combinations during early stages of graft development. Journal of Plant Physiology. 176: 25–35.
  • İşbilir, M.E., Saraçoğlu, O., Dinçer, E., Donat, A., Al-Salihi, A.A.M., 2022. Effects of paclobutrazol applications on rooting performance of black mulberry (Morus nigra L.) hardwood cuttings. Turkish Journal of Agriculture-Food Science and Technology. 10: 2722–2724.
  • Karimi, H.R., Nowrozy, M., 2017. Effects of rootstock and scion on graft success and vegetative parameters of pomegranate. Scientia Horticulturae. 214: 280–287.
  • Kawaguchi, M., Taji, A., Backhouse, D., Oda, M., 2008. Anatomy and physiology of graft incompatibility in solanaceous plants. Journal of Horticultural Science & Biotechnology. 83: 581–588.
  • Koç, M., 2011. Farklı köklenme ortam sıcaklığı ve nem değerinin karadut odun çeliklerinin köklenmesi üzerine etkileri. Gaziosmanpaşa Üniversitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 46 s.
  • Koyuncu, F., Şenel, E., 2003. Rooting of black mulberry (Morus nigra L.) hardwood cuttings. Journal of Fruit and Ornamental Plant Research. 11: 53–57.
  • Kurotani, K.I., Notaguchi, M., 2021. Cell-to-cell connection in plant grafting—molecular insights into symplastic reconstruction. Plant Cell Physiology. 62 (9): 1362–1371.
  • Lal, N., Ramteke, V., Diwan, G., et al., 2025. Physiological, biochemical, and molecular responses during grafting in horticultural crops. Plant Molecular Biology Reporter. 1–22.
  • Li, P., Sayre, K.D., 1975. The protein, non-protein, and total nitrogen in Solanum tuberosum spp. andigena potatoes. American Potato Journal. 52: 341–352.
  • Loupit, G., Cookson, S.J., 2020. Identifying molecular markers of successful graft union formation and compatibility. Frontiers in Plant Science. 11: 1903.
  • Luo, J., Wang, Y., Zhao, A.Z., 2019. The complete chloroplast genome of Morus alba (Moraceae: Morus), the herbal medicine species in China. Mitochondrial DNA Part B. 4 (2): 2467–2468.
  • MacAdam, J.W., Nelson, C.J., Sharp, R.E., 1992. Peroxidase activity in the leaf elongation zone of tall fescue: I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Physiology. 99 (3): 872–878.
  • Miao, L., Li, S.Z., Bai, L.Q., Anwar, A., Li, Y.S., He, C.X., Yu, X.C., 2019. Effect of grafting methods on physiological change of graft union formation in cucumber grafted onto bottle gourd rootstock. Scientia Horticulturae. 244: 249–256.
  • Mosse, B., 1962. Graft incompatibility in fruit trees. Technical communication no. 28, Comm. Bur. Horticulture and Plant Crops, East Malling.
  • Musacchi, S., Masia, A., Fachinello, J., 2002. Variation of some enzymatic activities in relationship to scion/stock compatibility in pear/quince combinations. Acta Horticulturae. 596: 389–392.
  • Nakano, Y., Asada, K., 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology. 22 (5): 867–880.
  • Nanda, A.K., Melnyk, C.W., 2018. The role of plant hormones during grafting. Journal of Plant Research. 131 (1): 49–58.
  • Narayan, P.S., Chakraborty, G., Subba, R., 1989. Regulation of plantlets from the callus of stem segment of mature plant of Morus alba L. Proceedings of the Indian National Science Academy. 55: 469–472.
  • Nicholson, R.L., Hammerschmidt, R., 1992. Phenolic compounds and their role in disease resistance. Annual Review of Phytopathology. 30: 369–389.
  • Özgür, M., Uçar, A., Yılmaz, S., 2025. The multifaceted benefits of Morus nigra L.: a pharmacological powerhouse. Phytochemistry Reviews. 24: 5317–5342.
  • Peng, C., Gao, F., Wang, H., Shen, H., Yang, L., 2020. Physiological and biochemical traits in Korean pine somatic embryogenesis. Forests. 11 (5): 577.
  • Peters, J.L., Castillo, F.J., Heath, R.L., 1989. Alteration of extracellular enzymes in pinto bean leaves upon exposure to air pollutants, ozone, and sulfur dioxide. Plant Physiology. 89 (1): 159–164.
  • Phillips, N., Reynolds, A., Di Profio, F., 2015. Nonstructural carbohydrate concentrations in dormant grapevine scionwood and rootstock impact propagation success and vine growth. HortTechnology. 25 (4): 536–550.
  • Quessada, M.P., 1984. Les Peroxidases de l'abricotier (Prunus armeniaca L.) et Leur Intervention Dans l'incompatibilité au Greffage. These 3ème cycle, Montpellier.
  • Regmi, S., Puri, C., Shah, K., 2022. IBA impact at different dose on mulberry cutting (Morus alba). Research in Agriculture, Livestock and Fisheries. 9 (3): 253–257.
  • Reig, G., Font, I., Forcada, C., Mestre, L., Jimenez, S., Betrán, J.A., Moreno, M.A., 2018. Horticultural leaf mineral and fruit quality traits of two ‘greengage’ plum cultivars budded on plum based rootstocks in Mediterranean conditions. Scientia Horticulturae. 232: 84–91.
  • Sivaci, A., 2006. Seasonal changes of total carbohydrate content in three varieties of apple (Malus sylvestris Miller) stem cuttings. Scientia Horticulturae. 109: 234–237.
  • Slinkard, S., Singleton, V.L., 1977. Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture. 28 (1): 49–55.
  • Su, W.C., He, H.Y., Liu, Z.Z., Mo, Z.H., Cao, F., Peng, F.R., 2021. Physiological and biochemical changes during graft union formation in Carya illinoinensis. Biologia Plantarum. 65 (2): 203–211.
  • Telles, C.A., Biasi, L.A., Mindêllo Neto, U.R., Deschamps, C., 2009. Fenóis totais, peroxidase e suas relações com a compatibilidade de mudas de pessegueiro interenxertadas. Ciência e Agrotecnologia. 33 (1): 86–91.
  • Uğur, R., 2025. Evaluation of suitable rootstock and key criteria for rootstock breeding in Prunus species. Applied Fruit Science. 67 (6): 475.
  • Uğur, R., Paydaş, S., Saridas, M.A., 2023. Rootstock breeding and rootstock-scion interaction in Prunus species. Erciyes Tarım ve Hayvan Bilimleri Dergisi. 6 (2): 7–10.
  • Ünal, A., Özçağıran, R., Hepaksoy, S., 1992. Kara dut ve mor dut çeşitlerinde odun çeliklerinin köklenmesi üzerinde bir araştırma. Türkiye I. Ulusal Bahçe Bitkileri Kongresi, 1: 267–270.
  • Vural, U., Dumanoğlu, H., Erdoğan, V., 2008. Effect of grafting/budding techniques and time on propagation of black mulberry (Morus nigra L.) in cold temperate zones. Propagation of Ornamental Plants. 8 (2): 55–58.
  • Yıldız, K., Çekiç, Ç., Güneş, M., Özgen, M., Özkan, Y., Akça, Y., Gerçekçioğlu, R., 2009. Farklı dönemlerde alınan karadut (Morus nigra L.) çelik tiplerinde köklenme başarısının belirlenmesi. GOÜ. Zir. Fak. Der. 26: 1–5.
  • Yılmaz, M., 1992. Bahçe Bitkileri Yetiştirme Tekniği. Ç.Ü. Basımevi, Adana.
  • Zarrouk, O., Testillano, P.S., Risueno, M.C., Moreno, M.A., Gogorcena, Y., 2010. Changes in cell/tissue organization and peroxidase activity as markers for early detection of graft incompatibility in peach/plum combinations. Journal of the American Society for Horticultural Science. 135: 9–17.
  • Zhang, J., Liu, Y., Li, C., Yin, B., Liu, X., Guo, X., Zhang, C., Liu, D., Hwang, I., Li, H., et al., 2022. PtomtAPX is an autonomous lignification peroxidase during the earliest stage of secondary wall formation in Populus tomentosa Carr. Nature Plants. 8 (7): 828–839.
Toplam 54 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ekoloji (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Duygu Özelçi 0000-0003-1621-1980

Gönderilme Tarihi 7 Kasım 2025
Kabul Tarihi 17 Aralık 2025
Yayımlanma Tarihi 24 Aralık 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 13 Sayı: 2

Kaynak Göster

APA Özelçi, D. (2025). Clonal Rootstock Potential of Morus spp. Genotypes and Their Biochemical Influence on Graft Compatibility in Morus nigra L. ÇOMÜ Ziraat Fakültesi Dergisi, 13(2), 352-362. https://doi.org/10.33202/comuagri.1819775