Elektroeğirme Yöntemi ile Üretilen Antimikrobiyal Nanolif Membranlarda Lycopodium Özütü Etkisinin İncelenmesi

Yıl 2024, Cilt: 39 Sayı: 4, 1027 - 1038, 25.12.2024

Ayşe Yüksekdağ

https://doi.org/10.21605/cukurovaumfd.1606113

Öz

Bu çalışmada, Lycopodium bitkisinden elde edilen fenolik bileşenler kullanılarak çevreci, ekonomik ve büyük ölçekli üretilebilen antimikrobiyal nanolif membranlar geliştirilmiştir. Bitki özütü, oda sıcaklığında polimer çözücüsü DMF ile karıştırılıp süzülerek elde edilmiştir. %12 PAN polimeri ile farklı konsantrasyonlarda Lycopodium özütü içeren çözeltiler hazırlanmış ve elektroeğirme yöntemiyle nanolif membranlar üretilmiştir. Üretilen dört farklı membran (%0, %1, %3, %5 oranlarında bitki özütü içeren membranlar) SEM görüntüleriyle incelenmiş, bitki konsantrasyonunun artışıyla boncuksu yapıların azaldığı ve nanolif kalınlıklarının arttığı gözlenmiştir. %5 oranında özüt içeren membranda nanolif kalınlığı 607 nm’ye çıkarak kontrol membranından yaklaşık üç kat daha kalın olmuştur. FT-IR analizinde 5L membranında oksijen bağlarını temsil eden piklerin kaybolduğu ve 3L membranında antimikrobiyal etkinin başarılı bir şekilde elde edildiği tespit edilmiştir.

Anahtar Kelimeler

Elektroeğirme, Nanolif, Lycopodium, Antimikrobiyal

Investigation of the Effect of Lycopodium Extract on Antimicrobial Nanofibrous Membranes Fabricated by Electrospinning Method

Öz

In this study, antimicrobial nanofiber membranes were developed using phenolic components obtained from the Lycopodium plant, which are environmentally friendly, economical, and suitable for large-scale production. The plant extract was mixed with the polymer solvent DMF at room temperature and then filtered to obtain the solution. Solutions containing 12% PAN polymer and varying concentrations of Lycopodium extract were prepared, and nanofiber membranes were produced using the electrospinning method. Four different membranes (membranes containing 0%, 1%, 3%, 5% plant extracts) were examined using SEM images, revealing a decrease in bead-like structures and an increase in nanofiber thickness with higher plant concentrations. The nanofiber thickness in the membrane having 5% plant extract increased to 607 nm, making it approximately three times thicker than the control membrane. FT-IR analysis showed that the peaks representing oxygen bonds were absent in the 5L membrane, and the 3L membrane demonstrated successful antimicrobial activity.

Anahtar Kelimeler

Electrospinning, Nanofiber, Lycopodium, Antimicrobial

Kaynakça

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