Öz
The aim of the present study was to investigate the effect of chitosan modification of silicon urethral catheters to improve antibacterial activity against to biofilm formation for long term catheterization. Chitosan was first immobilized on the silicon surface through a (3-aminopropyl) triethoxysilane (APTES) bridge in ethanol, toluene and acetone solutions, and glutaraldehyde used as cross linker. Fourier Transform Infrared Spectroscopy and Scanning Electron Microscopy were used for imaging surface modifications and characterization of silicone catheters. Biodegradability of chitosan hydrogel coatings was investigated with using artificial urea solution through pH measurements. The results showed that successful formation of chitosan hydrogels at catheter surface was observed both silicone urinary catheters
Anahtar Kelimeler
Kaynakça
- 1. Al-Aown, A., Kyriazis, I. et al. 2010. Ureteral Stents: New Ideas, New Designs, Therapeutic Advances in Urology, 2(2), 85.
- 2. Tunney, M.M., Gorman, S.P., 2002. Evaluation of Poly (Vinyl Pyrrolidone)-Coated Biomaterial for Urological use, Biomaterials, 23: 4601-4608.
- 3. Kowalczuk, D., Ginalska, G., Przekora, A., 2011. The Cytotoxicity Assessment of the Novel Latex Urinary Catheter with Prolonged Antimicrobial Activity, Journal of Biomedical Materials Research Vol 98(2) 222-228.
- 4. Xiaoying, L., We, İ.C., Yan, H., Yi, Z., Zhigong, W., 2009. Surface Modification on Silicon with Chitosan and Biological Research, Biomed. Mater. 4 (2009) 044103 (5pp).
- 5. Gunda, N.S.K., Singh, M., Norman, L., Kaur, K., Mitra, S.K., 2014. Optimization and Characterization of Biomolecule Immobilization on Silicon Substrates Using (3-aminopropyl) triethoxysilane (APTES) and Glutaraldehyde Linker, Applied Surface Science 305 (2014) 522–530.
- 6. Majoul, N., Aouida, S., Bessaïs, B., 2015. Progress of Porous Silicon APTES-Functionalization by FTIR Investigations, Applied Surface Science 331 (2015) 388–391.
Öz
Bu çalışmada, uzun dönem kateterizasyonda kullanılan silikon üriner kateterlerin biyofilm oluşumuna karşı antibakteriyel aktivitesini artırmada kitosanın etkisi incelenmiştir. Kitosan; etanol, toluen ve aseton kullanılarak hazırlanan (3-aminopropil) trietoksisilan (APTES) çözeltileri vasıtasıyla silikon yüzeye bağlanmıştır. Çapraz bağlayıcı olarak glutaraldehit kullanılmıştır. Yüzey karekterizasyonu için Fourier Transform Infrared Spectroscopy (FTIR) ve Scanning Electron Microscopy (SEM) kullanılmış, yapılan kitosan hidrojel kaplamaların biyobozunurlukları yapay üre sıvısında, sıvının pH değişimlerinin ölçülmesiyle incelenmiştir. Sonuç olarak, kullanılan silikon üriner kateterlerin üzerinde kitosan hidrojel kaplamaların başarılı bir şekilde oluşturulduğu gözlenmiştir.
Anahtar Kelimeler
Kaynakça
- 1. Al-Aown, A., Kyriazis, I. et al. 2010. Ureteral Stents: New Ideas, New Designs, Therapeutic Advances in Urology, 2(2), 85.
- 2. Tunney, M.M., Gorman, S.P., 2002. Evaluation of Poly (Vinyl Pyrrolidone)-Coated Biomaterial for Urological use, Biomaterials, 23: 4601-4608.
- 3. Kowalczuk, D., Ginalska, G., Przekora, A., 2011. The Cytotoxicity Assessment of the Novel Latex Urinary Catheter with Prolonged Antimicrobial Activity, Journal of Biomedical Materials Research Vol 98(2) 222-228.
- 4. Xiaoying, L., We, İ.C., Yan, H., Yi, Z., Zhigong, W., 2009. Surface Modification on Silicon with Chitosan and Biological Research, Biomed. Mater. 4 (2009) 044103 (5pp).
- 5. Gunda, N.S.K., Singh, M., Norman, L., Kaur, K., Mitra, S.K., 2014. Optimization and Characterization of Biomolecule Immobilization on Silicon Substrates Using (3-aminopropyl) triethoxysilane (APTES) and Glutaraldehyde Linker, Applied Surface Science 305 (2014) 522–530.
- 6. Majoul, N., Aouida, S., Bessaïs, B., 2015. Progress of Porous Silicon APTES-Functionalization by FTIR Investigations, Applied Surface Science 331 (2015) 388–391.
Ayrıntılar
| Bölüm | Makaleler |
|---|---|
| Yazarlar | |
| Yayımlanma Tarihi | 15 Ekim 2016 |
| Yayımlandığı Sayı | Yıl 2016 Cilt: 31 Sayı: ÖS2 |