Beneficial role of crocin against doxorubicin-induced testicular damage in rats: insights into vimentin modulation

Melike Özgül Önal; Sara Asaad; Gürkan Yiğittürk; Volkan Yaşar; Yasemin Biçer; Dilan Çetinavcı; Eyüp Altınöz; Mehmet Demir; Hülya Elbe; Feral Öztürk

doi:10.17826/cumj.1420141

EN TR

Beneficial role of crocin against doxorubicin-induced testicular damage in rats: insights into vimentin modulation

Abstract

Purpose: Doxorubicin (DOX) is a wide-spectrum antibiotic used for chemotherapy. Its side effects limit treatment. Crocin is one of the carotenoids that has both anti-inflammatory and antioxidant activities. We aimed to evaluate the effects of crocin against doxorubicin-induced testicular damage in rats. Materials and Methods: Forty Wistar rats were divided into four groups. Group 1: Control, Group 2: Crocin, Group 3: DOX, Group 4: DOX+Crocin (n=10, for all). Testis tissues were stained with Hematoxylin-Eosin. The diameters of seminiferous tubules were measured and the testicular mean histopathologic damage score (MHDS) was calculated. Vimentin expression in Sertoli cells was calculated as H-Score. Levels of Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT), and Superoxide dismutase (SOD) activities were determined in testis tissues. Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) were also calculated. Results: Atrophic seminiferous tubules were seen in the DOX group. Edema, vacuolization, and disorganization were present in the injured tubules. The MHDSs for the DOX group and control groups were 4.60±0.45 and 0.20±0.13, respectively. Both of these groups showed a significant difference. The histopathologic score was reduced after using crocin. Tubule damage considerably decreased while immunoexpression levels of vimentin and seminiferous tubule width significantly increased in the DOX+Crocin group compared to the DOX group. MDA and TOS levels were significantly increased after DOX treatment, and GSH, SOD, CAT, and TAS levels were significantly decreased. All biochemical indicators were greatly improved after receiving crocin. Conclusion: Crocin supplementation exhibited adequate beneficial effects against the testicular damage of DOX-induced function by balancing the oxidant/antioxidant status.

Keywords

Doxorubicin , crocin , testis , vimentin , oxidative stress.

Sıçanlarda doksorubisin kaynaklı testis hasarına karşı crocinin faydalı rolü: vimentin modülasyonuna ilişkin görüşler

Öz

Amaç: Doksorubisin (DOX) kemoterapide kullanılan geniş spektrumlu bir antibiyotiktir. Yan etkileri tedaviyi kısıtlamaktadır. Crocin, hem antiinflamatuar hem de antioksidan aktiviteye sahip karotenoidlerden biridir. Sıçanlarda doksorubisin kaynaklı testis hasarına karşı Crosinin etkilerini değerlendirmeyi amaçladık. Gereç ve Yöntem: Kırk adet Wistar sıçan dört gruba ayrıldı. Grup 1: Kontrol, Grup 2: Crosin, Grup 3: DOX, Grup 4: DOX+Crosin (tümü için, n=10). Testis dokuları Hematoksilen-Eozin ile boyandı. Seminifer tübüllerin çapları ölçüldü ve testisin ortalama histopatolojik hasar skoru (MHDS) ölçüldü. Sertoli hücrelerinde Vimentin ekspresyonu H-Score olarak hesaplandı. Testis dokularında Malondialdehit (MDA) ve Glutatyon (GSH), Katalaz (CAT) ve Süperoksit dismutaz (SOD) aktivitelerinin düzeyleri belirlendi. Toplam Antioksidan Durumu (TAS) ve Toplam Oksidan Durumu (TOS) da hesaplandı. Bulgular: DOX grubunda atrofik seminifer tübüller görüldü. Hasarlanan tübüllerde ödem, vakuolizasyon ve dezorganizasyon mevcuttu. DOX grubu ve kontrol grupları için MHDS'ler sırasıyla 4,60±0,45 ve 0,20±0,13 idi. Bu grupların her ikisi de anlamlı bir fark gösterdi. Crocin kullanımı sonrası histopatolojik hasar skoru azaldı. DOX grubuyla karşılaştırıldığında DOX+Crosin grubunda Vimentin immün ekspresyon düzeyleri ve seminifer tübül genişliği anlamlı derecede artarken tübül hasarı önemli ölçüde azaldı. DOX tedavisi sonrasında MDA ve TOS düzeyleri anlamlı düzeyde artarken, GSH, SOD, CAT ve TAS düzeyleri anlamlı düzeyde azaldı. Crocin aldıktan sonra tüm biyokimyasal göstergeler büyük ölçüde iyileşti. Sonuç: Crosin takviyesi, oksidan/antioksidan durumunu dengeleyerek DOX'un neden olduğu fonksiyonun testis hasarına karşı yararlı etkiler gösterdi.

Anahtar Kelimeler

Doksorubisin , Crosin , testis , vimentin , oksidatif stres.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Histoloji ve Embriyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Melike Özgül Önal
0000-0001-6710-5729
Türkiye

Sara Asaad
0000-0002-4315-3811
Türkiye

Gürkan Yiğittürk
0000-0002-5315-253X
Türkiye

Volkan Yaşar
0000-0003-3497-1238
Türkiye

Yasemin Biçer
0000-0001-6032-1234
Türkiye

Dilan Çetinavcı
0000-0002-4148-7711
Türkiye

Eyüp Altınöz
0000-0002-3991-9773
Türkiye

Mehmet Demir
0000-0003-0593-2205
Türkiye

Hülya Elbe ^*
0000-0002-1254-0683
Türkiye

Feral Öztürk
0000-0003-1207-5213
Türkiye

Yayımlanma Tarihi

30 Haziran 2024

Gönderilme Tarihi

16 Ocak 2024

Kabul Tarihi

7 Nisan 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 49 Sayı: 2

DOI

https://doi.org/10.17826/cumj.1420141

IZ

https://izlik.org/JA27LC98WG

MLA

Özgül Önal, Melike, vd. “Beneficial role of crocin against doxorubicin-induced testicular damage in rats: insights into vimentin modulation”. Cukurova Medical Journal, c. 49, sy 2, Haziran 2024, ss. 332-4, doi:10.17826/cumj.1420141.

Bu eser Creative Commons Atıf-Gayriticari-Türetilemez 4.0 Uluslararası Lisansı ile lisanslanmıştır.

Beneficial role of crocin against doxorubicin-induced testicular damage in rats: insights into vimentin modulation

Abstract

Keywords

Sıçanlarda doksorubisin kaynaklı testis hasarına karşı crocinin faydalı rolü: vimentin modülasyonuna ilişkin görüşler

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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