Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri

Büşra Şen Halıcıoğlu; Mehmet İbrahim Tuğlu

doi:10.17826/cumj.852402

EN TR

Effects of adipose tissue derived mesenchymal stem cells and conditioned medium on experimental premature ovarian insufficiency model

Öz

Purpose: The aim of this study was to investigate the effects of adipose tissue derived mesenchymal stem cells (ADMSC) and conditioned medium (CM), which can be recommended for the treatment of chemotherapy-induced premature ovarian insufficiency (POI), on oxidative stress and apoptosis. Materials and Methods: In the study, adult female Wistar albino rats were divided into 4 groups; Control, POI, POI+ADMSC, POI+CM. H&E staining was performed for histopathological evaluations in the ovary and all ovarian follicles were counted. iNOS and Caspase3 expressions were shown immunohistochemically as markers of oxidative stress and apoptosis. Results: Follicular cell degenerations and vacuolization were observed in the ovaries of the POI group, while significant decreases were observed in these degenerations in the ADMSC and CM groups. Increased iNOS and Caspase3 expressions in the POI group were statistically significant decreased in both the ADMSC and CM groups. Conclusion: Mesenchymal stem cells and cell-free conditioned medium, which are increasingly used in the field of regenerative medicine, can be an alternative treatment strategy for POI by reducing apoptosis and oxidative stress, which are the main mechanisms of the disease.

Anahtar Kelimeler

premature ovarian insufficiency, adipose derived mesenchymal stem cell, conditioned medium, apoptosis, oxidative stress

Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri

Öz

Amaç: Kemoterapi kaynaklı prematür over yetmezliği (POY) tedavisi için önerilebilecek yağ doku mezenkimal kök hücreleri (YDMKH) ve bu hücrelerden elde edilmiş koşullu besiyerinin (KB) oksidatif stres ve apoptoz üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Çalışmada Wistar albino cinsi erişkin dişi sıçanlar 4 gruba ayrıldı. Kontrol, POY, POY+YDMKH, POY+KB. Ovaryumda histopatolojik değerlendirmeler için H&E boyamaları yapıldı ve tüm ovaryum folilkülleri sayıldı. Oksidatif stres ve apoptoz belirteci olarak iNOS ve Caspase3 ekspresyonları immunohistokimyasal olarak gösterildi. Bulgular: Analizler sonucu POY grubu ovaryumlarında foliküller hücre dejenerasyonları, vakuolizasyon, gibi dejenerasyonlar gözlenirken, YDMKH ve KB gruplarında bu dejenerasyonlarda anlamlı azalmalar görüldü. POY grubunda artmış olan iNOS ve Caspase3 ekspresyonları YDMKH ve KB gruplarının her ikisinde de istatistiksel olarak anlamlı şekilde azaldı. Sonuç: Rejeneratif tıp alanında her geçen gün kullanımı artan mezenkimal kök hücrelerin ve hücresiz koşullu besiyerinin prematür over yetmezliğinde hastalığın temel mekanizmalarından olan apoptoz ve oksidatif stresin azaltılması yoluyla, POY için alternatif bir tedavi stratejisi olabileceği gösterildi.

Anahtar Kelimeler

prematür over yetmezliği, mezenkimal kök hücre, koşullu besiyeri, apoptoz, oksidatif stres

Destekleyen Kurum

Manisa Celal Bayar Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

2019-021

Teşekkür

Bu çalışma, Manisa Celal Bayar Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 2019-021 numaralı proje ile desteklenmiştir

Kaynakça

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39. Halıcıoğlu BŞ. Farkli deneysel prematür over yetmezlİğİ modellerİnde kök hücre tedavİsİnİn etkİnlİğİ [PhD thesis]. Manisa: Manisa Celal Bayar University; 2020.

Ayrıntılar

Birincil Dil

Türkçe

Konular

Klinik Tıp Bilimleri, Klinik Tıp Bilimleri (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Büşra Şen Halıcıoğlu ^*
0000-0003-4089-5243
Türkiye

Mehmet İbrahim Tuğlu
0000-0002-0569-8415
Türkiye

Yayımlanma Tarihi

30 Haziran 2021

Gönderilme Tarihi

13 Ocak 2021

Kabul Tarihi

26 Şubat 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 46 Sayı: 2

DOI

https://doi.org/10.17826/cumj.852402

IZ

https://izlik.org/JA63XN86LG

APA

Şen Halıcıoğlu, B., & Tuğlu, M. İ. (2021). Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri. Cukurova Medical Journal, 46(2), 523-531. https://doi.org/10.17826/cumj.852402

AMA

1.Şen Halıcıoğlu B, Tuğlu Mİ. Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri. Cukurova Med J. 2021;46(2):523-531. doi:10.17826/cumj.852402

Chicago

Şen Halıcıoğlu, Büşra, ve Mehmet İbrahim Tuğlu. 2021. “Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri”. Cukurova Medical Journal 46 (2): 523-31. https://doi.org/10.17826/cumj.852402.

EndNote

Şen Halıcıoğlu B, Tuğlu Mİ (01 Haziran 2021) Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri. Cukurova Medical Journal 46 2 523–531.

IEEE

[1]B. Şen Halıcıoğlu ve M. İ. Tuğlu, “Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri”, Cukurova Med J, c. 46, sy 2, ss. 523–531, Haz. 2021, doi: 10.17826/cumj.852402.

ISNAD

Şen Halıcıoğlu, Büşra - Tuğlu, Mehmet İbrahim. “Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri”. Cukurova Medical Journal 46/2 (01 Haziran 2021): 523-531. https://doi.org/10.17826/cumj.852402.

JAMA

1.Şen Halıcıoğlu B, Tuğlu Mİ. Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri. Cukurova Med J. 2021;46:523–531.

MLA

Şen Halıcıoğlu, Büşra, ve Mehmet İbrahim Tuğlu. “Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri”. Cukurova Medical Journal, c. 46, sy 2, Haziran 2021, ss. 523-31, doi:10.17826/cumj.852402.

Vancouver

1.Büşra Şen Halıcıoğlu, Mehmet İbrahim Tuğlu. Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri. Cukurova Med J. 01 Haziran 2021;46(2):523-31. doi:10.17826/cumj.852402

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Effects of adipose tissue derived mesenchymal stem cells and conditioned medium on experimental premature ovarian insufficiency model

Öz

Anahtar Kelimeler

Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri

Öz

Anahtar Kelimeler

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Kaynakça

Ayrıntılar

Birincil Dil

Konular

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