Antioxidant activities of inula viscosa extract and curcumin on U87 cells induced by beta-amyloid

Ares Alizade; Gülüzar Özbolat

doi:10.17826/cumj.854213

TR EN

Beta-amiloid ile indüklenen U87 hücrelerinde inula viscosa ekstresi ve kurkuminin antioksidan aktiviteleri

Öz

Amaç: Bu çalışmada, Inula viscosa ekstresi ve Kurkumin'in Alzheimer hastalığı (AD) model hücre dizisi olan amiloid-beta (Aβ), ile tedavi edilen U87 (insan astrositom hücre dizisi) üzerindeki etkilerini araştırmayı amaçladık. Gereç ve Yöntem: İlk olarak, U87 hücrelerinde inula ve kurkuminin sitotoksik potansiyeli kolorimetrik MTT (3-4,5-dimetil-tiyazolil-2,5-difeniltetrazolyum bromür) testi ile araştırıldı. Daha sonra Total Glutatyon miktarı, Malondialdehit (MDA), Glutatyon redüktaz (GR) aktiviteleri araştırıldı. Inula viscosa ve Curcumin ile muamele edilmiş U87 hücre hatlarında bölünmüş Bax ve Bcl-2 proteinlerinin ekspresyonunu ve aktivitesini incelemek için ELISA testi kullanıldı. Bulgular: Inula viscosa ve Kurkumin muamelesinin, hücrelerde amiloid-B'nin neden olduğu hücre ölümünü azalttı. Ayrıca, proapoptotik protein Bax ve Bcl-2'nin aktivasyonunu azaltırken amiloid-B'nin neden olduğu oksidatif stresi de azalttı. Sonuç: Sonuçlarımız, inula viscosa'nın Alzheimer tedavisinde yeni bir yaklaşımı temsil edebileceğini göstermektedir.

Anahtar Kelimeler

ınula viscosa , curcumin , U87 , Alzheimer

Antioxidant activities of inula viscosa extract and curcumin on U87 cells induced by beta-amyloid

Abstract

Purpose: The aim of this study was to investigate the effects of Inula viscosa extract and Curcumin on the U87 (human astrocytoma cell line) treated with amyloid-beta (Aβ), which is the Alzheimer’s disease (AD) model cell line. Materials and Methods: Firstly, the cytotoxic potential of ınula and curcumin was investigated in the U87 cells by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide) assay. Then, the amount of Total Glutathione, Malondialdehyde (MDA), Glutathione reductase (GR) activities were investigated. ELISA test was used to examine the expression and activity of cleaved Bax and Bcl-2 proteins in the Inula viscosa and Curcumin treated U87 cell lines. Results: Inula viscosa and Curcumin treatment reduced cell death caused by amyloid-B in cells. It also reduced the oxidative stress caused by amyloid-B, while reducing the activation of the proapoptotic protein Bax, and Bcl-2. Conclusion: Our results suggest that inula viscosa may represent a new approach in the treatment of Alzheimer's.

Keywords

ınula viscosa , curcumin , Alzheimer , U87

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Klinik Tıp Bilimleri

Bölüm

Araştırma Makalesi

Yazarlar

Ares Alizade ^*
0000-0003-0334-8152
Türkiye

Gülüzar Özbolat
0000-0002-3487-1088
Türkiye

Yayımlanma Tarihi

30 Haziran 2021

Gönderilme Tarihi

5 Ocak 2021

Kabul Tarihi

3 Nisan 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 46 Sayı: 2

DOI

https://doi.org/10.17826/cumj.854213

IZ

https://izlik.org/JA98ZG89TC

MLA

Alizade, Ares, ve Gülüzar Özbolat. “Antioxidant activities of inula viscosa extract and curcumin on U87 cells induced by beta-amyloid”. Cukurova Medical Journal, c. 46, sy 2, Haziran 2021, ss. 583-8, doi:10.17826/cumj.854213.