Yıl 2020, Cilt 45 , Sayı 2, Sayfalar 541 - 546 2020-06-30

L-carnitine effects in CCl4-nephrotoxicity: Immunohistochemical evaluation of glomerular nephrin and HIF-1alpha expressions
CCl4-nefrotoksisitesinde L-karnitin’in etkileri: Glomeruler nefrin ve HIF-1alfa ekspresyonlarının immunohistokimyasal değerlendirilmesi

Derya KARABULUT [1] , Emel ÖZTÜRK [2] , Ali Tuğrul Tuğrul AKİN [3] , Ayça LEKESİZCAN [4] , Hacı Murat Murat ÜNSAL [5] , Tuğçe Merve ÖZYAZGAN [6] , Meryem SAYAN [7]


Purpose: In this study, we aimed to demonstrate the effects of L-carnitine after carbontetrachloride (CCl4) toxicity through nephrin and Hypoxia inducible factor-1 alpha (HIF-1α) expressions in the glomerular structure.
Materials and Methods: Forty male Sprague dawley rats were divided into 5 groups with animals in each group. Group I: Control group; 0.2 ml olive oil intraperitoneal (ip) twice weekly, Group II: L-carnitine group; 200 mg/kg L-carnitine (ip) twice a week, Group III: CCl4 group; 0.2 ml CCl4 (ip) twice a week for 6 weeks, Group IV: L-carnitine + CCl4 group, 200 mg/kg ip L-carnitine 24 hours before CCl4 twice a week, Group V: CCl4 + L-carnitine group; 200 mg/kg L-carnitine half an hour after CCl4 twice a week. Immunohistochemical staining was performed on kidney tissue sections to show nephrin and HIF-1α expression. Expression densities of the proteins were measured by ImageJ program.
Results: Nephrin expression was significantly increased in Group III compared to other groups. There was a significant increase in HIF-1α expression only between Group I and Group III. Expression densities of proteins in L-carnitine-treated groups were similar to control.
Conclusion: L-carnitine has both protective and therapeutic effects against CCl4 toxicity in renal glomeruli.
Amaç: Bu çalışmada glomerül yapısındaki nefrin ve hipoksi indüklenebilir faktör-1alfa ekspresyonları aracılığıyla karbontetraklorid (CCl4) toksisitesi sonrası L-karnitin’in etkilerinin gösterilmesini amaçladık.
Gereç ve Yöntem: 40 adet Sprague dawley erkek sıçan 5 gruba (n=8) ayrıldı. Grup I: Kontrol grubu; 0.2 ml zeytinyağı intraperitoneal (ip) haftada 2 kez, Grup II: L-karnitin grubu; 200 mg/kg L-karnitin (ip) haftada 2 kez, Grup III: CCl4 group; 0.2 ml CCl4 (ip) haftada 2 kez 6 hafta boyunca, Grup IV: L-karnitin + CCl4 grubu, haftada 2 kez CCl4 uygulamasından önce 200 mg/kg ip L-karnitin, Grup V: CCl4 + L-karnitin grubu, haftada 2 kez CCl4 uygulamasından 1 saat sonra 200 mg/kg ip L-karnitin. Böbrek doku kesitlerine nefrin ve HIF-1α ekspresyonunu göstermek için immunohistokimya boyama uygulandı. Proteinlerin ekspresyon yoğunlukları ImageJ programında ölçüldü.
Bulgular: Nefrin ekspresyonu diğer gruplar ile kıyaslandığında Grup III’de anlamlı olarak arttı. HIF-1α ekspresyonu yalnızca Grup I ve Grup III arasında anlamlı şekilde arttı. Proteinlerin ekspresyon yoğunlukları L-karnitin-tedavili gruplarda kontrol grubuna benzerdi.
Sonuç: L-karnitin böbrek glomerulusunda CCl4 toksisitesine karşı hem koruyucu hem tedavi edici etkilere sahiptir.
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Birincil Dil en
Konular Anatomi ve Morfoloji, Hücre Biyolojisi, Üroloji ve Nefroloji
Bölüm Araştırma
Yazarlar

Orcid: 0000-0003-2067-6174
Yazar: Derya KARABULUT (Sorumlu Yazar)
Kurum: ERCIYES UNIVERSITY
Ülke: Turkey


Orcid: 0000-0003-0756-0329
Yazar: Emel ÖZTÜRK
Kurum: HARRAN UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-1408-8571
Yazar: Ali Tuğrul Tuğrul AKİN
Kurum: ERCIYES UNIVERSITY
Ülke: Turkey


Orcid: 0000-0003-2586-4756
Yazar: Ayça LEKESİZCAN
Kurum: ERCIYES UNIVERSITY
Ülke: Turkey


Orcid: 0000-0001-8234-6571
Yazar: Hacı Murat Murat ÜNSAL
Kurum: ERCIYES UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-4145-6907
Yazar: Tuğçe Merve ÖZYAZGAN
Kurum: ERCIYES UNIVERSITY
Ülke: Turkey


Orcid: 0000-0002-9068-1094
Yazar: Meryem SAYAN
Kurum: ERCIYES UNIVERSITY
Ülke: Turkey


Destekleyen Kurum Erciyes University BAP Unit
Proje Numarası TSA-2018-8227
Tarihler

Kabul Tarihi : 25 Mart 2020
Yayımlanma Tarihi : 30 Haziran 2020

MLA Karabulut, D , Öztürk, E , Aki̇n, A , Lekesi̇zcan, A , Ünsal, H , Özyazgan, T , Sayan, M . "L-carnitine effects in CCl4-nephrotoxicity: Immunohistochemical evaluation of glomerular nephrin and HIF-1alpha expressions" . Cukurova Medical Journal 45 (2020 ): 541-546 <https://dergipark.org.tr/tr/pub/cumj/issue/53180/674044>