Yıl 2020, Cilt 45 , Sayı 3, Sayfalar 769 - 777 2020-09-30

Genetik absans epilepsili WAG/Rij sıçanlarda kardiyovasküler değişiklikler: Kronik etosüksimid tedavisinin etkileri
Cardiovascular changes in genetic absence epileptic WAG/Rij rats: Effects of chronic ethosuximide treatment

Tuğçe DEMİRTAŞ ŞAHİN [1] , Tijen UTKAN [2] , Ayşe KARSON [3] , Yusufhan YAZIR [4] , Erdal KARAOZ [5]


Amaç: Bu çalışmada, genetik absans epilepsili WAG/Rij sıçanlarda kronik etosüksimid (ETS) tedavisinin absans nöbetler ve gözlenebilecek olası kardiyovasküler değişiklikler üzerindeki etkilerinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: İki aylık erkek Wistar ve WAG/Rij sıçanlar; Wistar kontrol, Wistar ETS, WAG/Rij kontrol ve WAG/Rij ETS gruplarına ayrılmıştır (n=20). ETS grupları 3 ay boyunca kronik ETS tedavisi (oral, 300 mg/kg/gün) almışlardır. Üç aylık tedavi periyodunun sonunda EEG kayıtları kullanılarak diken dalga deşarjlarının (DDD) toplam süre, ortalama süre ve sayısı değerlendirilmiştir. Ortalama arteriyel kan basıncı (OAB) ve kalp hızı (KH) ölçümleri gerçekleştirilmiştir.
Bulgular: ETS tedavisi WAG/Rij sıçanlarda DDD’leri toplam süre, ortalama süre ve sayı açısından anlamlı olarak azaltmıştır. OAB, WAG/Rij kontrol grubunda Wistar kontrol grubuna göre anlamlı olarak artmıştır. KH, Wistar ETS grubunda Wistar kontrol grubuna göre anlamlı olarak azalmıştır. KCl aracılı kasılma yanıtlarında Wistar kontrol grubuna göre Wistar ETS grubunda anlamlı artma, WAG/Rij kontrol grubunda anlamlı azalma saptanmıştır.
Sonuç: Sonuçlarımız OAB ve vasküler reaktivitenin WAG/Rij sıçanlarda arttığını göstermiştir. ETS tedavisi, WAG/Rij sıçanlarda kardiyovasküler parametreleri değiştirmezken Wistar sıçanlarda KH ve vasküler reaktiviteyi azaltmış OAB’yi etkilememiştir. Bu değişikliklerde T-tipi Ca++ kanallarının rol oynayabileceği düşünülmektedir.
Purpose: The aim of this study was to investigate the effects of chronic ethosuximide (ETX) treatment on absence seizures and cardiovascular parameters in WAG/Rij rats with genetic absence epilepsy.
Materials and Methods: Eight-weeks old, male Wistar and WAG/Rij rats were divided into four groups (n=20): Wistar control, Wistar ETX, WAG/Rij control and WAG/Rij ETX. ETX groups received chronic ETX treatment (oral, 300 mg/kg/day) for 3 months. At the end of the 3-month-treatment period; the total and mean duration, also number of spike wave discharges (SWDs) were evaluated using EEG recordings. Mean arterial blood pressure (MAP) and heart rate (HR) measurements were performed.
Results: ETX treatment significantly decreased the duration and frequency of SWDs in WAG/Rij rats. MAP in WAG/Rij control group was markedly higher than Wistar control group. In Wistar ETX group, HR was significantly slower than Wistar control group. KCl-induced contraction response enhanced in Wistar ETX group and diminished in WAG/Rij control group compared to Wistar control group.
Conclusion: Increased MAP and vascular reactivity in WAG/Rij rats. ETX treatment did not alter cardiovascular parameters in WAG/Rij rats whereas the treatment decreased the HR and vascular reactivity without affecting MAP in Wistar rats. T-type Ca++ channels may play a role in these changes. 
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Birincil Dil en
Konular Tıbbi Araştırmalar Deneysel
Bölüm Araştırma
Yazarlar

Orcid: 0000-0002-9158-7294
Yazar: Tuğçe DEMİRTAŞ ŞAHİN (Sorumlu Yazar)
Kurum: Kocaeli Universitesi Tıp Fakültesi Tıbbi Farmakoloji ABD
Ülke: Turkey


Orcid: 0000-0001-5848-3680
Yazar: Tijen UTKAN
Kurum: KOCAELİ ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0003-4909-4012
Yazar: Ayşe KARSON
Kurum: KOCAELİ ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-8472-0261
Yazar: Yusufhan YAZIR
Kurum: KOCAELİ ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0002-9992-833X
Yazar: Erdal KARAOZ
Kurum: İSTİNYE ÜNİVERSİTESİ
Ülke: Turkey


Destekleyen Kurum TÜBİTAK
Proje Numarası SBAG / 114S481
Tarihler

Kabul Tarihi : 27 Mayıs 2020
Yayımlanma Tarihi : 30 Eylül 2020

MLA Demi̇rtaş Şahi̇n, T , Utkan, T , Karson, A , Yazır, Y , Karaoz, E . "Cardiovascular changes in genetic absence epileptic WAG/Rij rats: Effects of chronic ethosuximide treatment" . Cukurova Medical Journal 45 (2020 ): 769-777 <https://dergipark.org.tr/tr/pub/cumj/issue/54246/724491>