Gölgede Kurutulmuş Althaea officinalis L. Çiçeklerinin GC–MS Tabanlı Uçucu Bileşen Profili

Öz

Althaea officinalis L. (hatmi), Malvaceae familyasına ait tıbbi ve süs değeri taşıyan bir bitki olup özellikle Doğu Akdeniz’de yaygın olarak yetiştirilmektedir. Çiçekleri, yüzyıllardır ateş düşürücü, yumuşatıcı ve kanamayı durdurucu özellikleri nedeniyle geleneksel tedavide kullanılmaktadır. Bu çalışmada, hatmi çiçeklerinin uçucu bileşen profili ilk kez detaylı olarak incelenmiştir. Araştırma kapsamında, A. officinalis çiçeklerinden purge and trap yöntemi ile elde edilen ekstraktın uçucu bileşenleri GC-MS ile analiz edilmiştir. Toplam 24 uçucu bileşik tanımlanmış olup bunlar arasında sekiz terpenoid, altı alkol, beş asit, bir kükürt bileşiği, bir keton, bir uçucu fenol, bir ester ve bir lakton yer almaktadır. Bitkisel aromalarda yaygın olarak görülen terpenoidler, bileşiklerin büyük çoğunluğunu oluşturmuştur. Baskın bileşenler arasında pulegon (%32.17), 3-penten-2-ol (%8.69), 4-etoksi etil benzoat (%6.68), 2-hekzanol (%6.55) ve asetik asit (%6.23) öne çıkmıştır. Çalışmada pulegon, çiçeğin baskın bileşeni olarak belirlenmiş olup mentol biyosentezinde ara ürün olması ve karakteristik nane benzeri kokusu açısından önem taşımaktadır. Elde edilen sonuçlar, hatmi çiçeklerinin aromatik bileşenlerinin hem duyusal özellikler hem de biyolojik aktiviteler açısından potansiyel önem taşıdığını göstermektedir.

Anahtar Kelimeler

• Althaea officinalis
• uçucu bileşikler
• GC-MS
• terpenoidler
• pulegon

GC–MS Based Profiling of Volatile Constituents in Shade-Dried Althaea officinalis L. Flowers

Öz

Althaea officinalis L., commonly known as marshmallow, is a medicinal and ornamental plant belonging to the Malvaceae family and is widely cultivated in the Eastern Mediterranean region. Its flowers have traditionally been used for their antipyretic, emollient, and styptic properties. The present study provides the first comprehensive report on the volatile composition of marshmallow flowers. Volatile constituents of A. officinalis flower extract were isolated using the purge and trap technique and analysed by gas chromatography–mass spectrometry (GC–MS). A total of 24 volatile compounds were identified, including eight terpenoids, six alcohols, five acids, one sulphur-containing compound, one ketone, one volatile phenol, one ester, and one lactone. Terpenoids represented the dominant chemical group, consistent with other aromatic plants. The major components were pulegone (32.17%), 3-penten-2-ol (8.69%), 4-ethoxy ethyl benzoate (6.68%), 2-hexanol (6.55%), and acetic acid (6.23%), accompanied by minor amounts of higher alcohols, fatty acids and related compounds. Pulegone was identified as the principal constituent, notable for its mint-like aroma and its role as an intermediate in (-)-menthol biosynthesis. The findings highlight the aromatic profile and potential biological relevance of A. officinalis flowers, contributing novel data to the phytochemical characterisation of this species.

Anahtar Kelimeler

• Althaea officinalis
• volatile compounds
• GC-MS
• terpenoids
• pulegone

Kaynakça

1. Al-Snafi, A. E. (2013). The pharmaceutical importance of Althaea officinalis and Althaea rosea: A review. International Journal of PharmTech Research, 5(3), 1387-1385.
2. Božović, M., & Ragno, R. (2017). Calamintha nepeta (L.) Savi and its main essential oil constituent pulegone: Biological activities and chemistry. Molecules, 22(2), 290.
3. Curnow, A., & Owen, S. J. (2016). An evaluation of root phytochemicals derived from Althea officinalis (Marshmallow) and astragalus membranaceus as potential natural components of UV protecting dermatological formulations. Oxidative medicine and cellular longevity, 2016(1), 7053897.
4. Dagli, M. M., Yeldan, P. A., Sevindik, O., Guclu, G., Kelebek, H., & Selli, S. (2022). Investigation of aroma compounds of queen anne’s pocket melon (Cucumis melo L. ssp. dudaim) juice. Journal of Raw Materials to Processed Foods, 3(2), 74-82.
5. Jordan, M. J., Margaria, C. A., Shaw, P. E., & Goodner, K. L. (2002). Aroma active components in aqueous kiwi fruit essence and kiwi fruit puree by GC-MS and multidimensional GC/GC-O. Journal of Agricultural and Food Chemistry, 50(19), 5386-5390.
6. Kalam, M. A., Ahmad, R., Avid, M., Rahim, A., & Habib, A. (2023). Khatmī (Althea officinalis L. and Althea rosea): Medicinal importance in the perspective of Unani medicine and pharmacological studies. Journal of Drug Delivery & Therapeutics, 13(12), 270-277.
7. Khalid, M. U., Phyo, H. M., Du, Y., Ali, K., Ashraf, W., Yu, H., ... & Yao, W. (2024). Synergistic inhibition effect of anisaldehyde and cinnamaldehyde (SAC) against Aspergillus species (A. flavus & A. niger) in vitro and on bread. Food Bioscience, 61, 104607.
8. Kozuharova, E., Simeonov, V., Batovska, D., Stoycheva, C., Valchev, H., & Benbassat, N. (2023). Chemical composition and comparative analysis of lavender essential oil samples from Bulgaria in relation to the pharmacological effects. Pharmacia, 70, 395-403.

9. Li, J., Wen, R., Wang, J., Zou, T., Zhang, D., Hou, Y., ... & Shi, J. (2024). Identification of key aroma-active compounds in long shelf-life yoghurt and milk produced by different pre-sterilisation processes of raw milk using SBSE-GC× GC–O–MS. International Journal of Food Science and Technology, 59(6), 3667-3678.
10. Liu, C., Gao, Q., Shang, Z., Liu, J., Zhou, S., Dang, J., ... & Lin, W. (2021). Functional characterization and structural insights into stereoselectivity of pulegone reductase in menthol biosynthesis. Frontiers in Plant Science, 12, 780970.
11. Maslov Bandić, L., Oštarić, F., Vinceković, M., & Mikulec, N. (2023). Biochemistry of aroma compounds in cheese. Mljekarstvo: časopis za unaprjeđenje proizvodnje i prerade mlijeka, 73(4), 211-224.
12. Naz, R., Anis, M., & Alatar, A. A. (2017). Embling production in Althaea officinalis L., through somatic embryogenesis and their appraisal via histological and scanning electron microscopical studies. Applied biochemistry and biotechnology, 182(3), 1182-1197.
13. Ozturk, M., Altay, V., Gucel, S., & Altundag, E. (2017). Plant diversity of the drylands in Southeastern Anatolia-Turkey: role in human health and food security. In Plant biodiversity: monitoring, assessment and conservation (pp. 83-124). Wallingford UK: CABI.
14. Oussou, K. F., Guclu, G., Sevindik, O., Kelebek, H., Starowicz, M., & Selli, S. (2022). GC-MS-Olfactometric Characterization of volatile and key odorants in Moringa (Moringa oleifera) and Kinkeliba (Combretum micranthum G. Don) herbal tea infusions prepared from cold and hot brewing. Separations, 10(1), 10.
15. Paul, A., Mishra, S. S., Maji, A., Samanta, A., Nahar, S., & Maity, T. K. (2025). Exploring the therapeutic potentials of cuminaldehyde: a comprehensive review of biological activities, mechanisms, and novel delivery systems. Phytochemistry Reviews, 1-32.
16. Rani, S., Khan, S. A., & Ali, M. (2010). Phytochemical investigation of the seeds of Althea officinalis L. Natural product research, 24(14), 1358-1364.
17. Sevindik, O., Kelebek, H., Rombola, A. D., & Selli, S. (2022). Grape seed oil volatiles and odour activity values: A comparison with Turkish and Italian cultivars and extraction methods. Journal of Food Science and Technology, 59(5), 1968-1981.
18. Sleha, R., Mosio, P., Vydrzalova, M., Jantovska, A., Bostikova, V., & Mazurova, J. (2014). In vitro antimicrobial activities of cinnamon bark oil, anethole, carvacrol, eugenol and guaiazulene against Mycoplasma hominis clinical isolates. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 158(2), 208-11.
19. Ulbricht, C., Basch, E., Ulbricht, C., Hammerness, P., & Vora, M. (2003). Marshmallow (Althaea officinalis L.) monograph. Journal of Herbal Pharmacotherapy, 3(3), 71-81.
20. Wang, Z. J., & Heinbockel, T. (2018). Essential oils and their constituents targeting the GABAergic system and sodium channels as treatment of neurological diseases. Molecules, 23(5), 1061.
21. Xue, T. T., Xu, H. B., Tang, Z. S., Duana, J. A., Liu, H. B., Shi, X. B., & Song, Z. X. (2021). Progress in chemical compositions and pharmacological activities of Althaea officinalis. Med Res, 5(2), 210002.
22. Zhang, Y., Li, X., Lo, C. K., & Guo, S. T. (2010). Characterization of the volatile substances and aroma components from traditional soypaste. Molecules, 15(5), 3421-3427.
23. Zhang, Y., Xiong, Y., An, H., Li, J., Li, Q., Huang, J., & Liu, Z. (2022). Analysis of volatile components of jasmine and jasmine tea during scenting process. Molecules, 27(2), 479.