Production and characterization of porous bacterial cellulose for skin substitution

Öz

Bacterial cellulose (BC), is a natural polymer produced by certain microorganisms, which is biocompatible, easy to manufacture, has high tensile strength, and has high water retention with its nanofibril network structure. BC is a good candidate for medical applications, and also has potential in skin substitution studies thanks to its membrane structure. However, its tight cellulose nanofibers do not allow cell attachment and migration. In this study, in situ keratin modified production, and characterization of BC with sufficient pore diameters that can be potential skin substitute was aimed. BC has been produced by Gluconacetobacter xylinus ATCC 700178 strain using two different methods (agar shredding and agar dropping) to increase the pore diameter between cellulose nanofibrils using and modified with keratin as an important component of the skin. Human hair was used as the source of keratin and keratin was obtained according to the Shindai extraction method. Keratin solution was impregnated with BC membranes and the characterization of the material was carried out by FTIR (Fourier Transform Infrared Spectrometry), SEM (Scanning Electron Microscope) and physical strength tests. As a result, the production of skin replacement candidate BC, containing keratin in its structure with sufficient pore diameter (>100μm) with a tensile strength of the range between 0.1- 0.15 MPa, was produced successfully.

Anahtar Kelimeler

• Bacterial cellulose
• keratin
• skin
• biocomposite materials

Proje Numarası

FYL-2018-20231

Deri ikamesi için gözenekli bakteriyel selüloz üretimi ve karakterizasyonu

Öz

Bakteriyel selüloz (BS), biyouyumlu, üretimi kolay, yüksek çekme dayanımı gösteren, nanofibril ağ yapısı ile yüksek su tutma özelliğinde olan ve bazı mikroorganizmalar tarafından üretilebilen doğal bir polimerdir. Medikal uygulamalar için iyi bir aday olan BS, membran yapısı nedeniyle deri ikamesi çalışmalarında geliştirilebilir bir potansiyel taşımaktadır. Ancak, sıkı yapıdaki selüloz nanofibrilleri hücre tutunması ve göçüne imkân vermemektedir. Bu çalışma kapsamında deri ikamesi olarak kullanılabilecek yeterli gözenek çapına sahip BS’nin in situ üretimi, keratin ile modifikasyonu ve karakterizasyonu amaçlanmıştır. Gluconacetobacter xylinus ATCC 700178 suşu kullanılarak iki farklı yöntemle (agar parçalama ve agar damlatma) selüloz nanofibrilleri arasındaki gözenek çapı arttırılarak üretilen BS daha sonra derinin önemli bir bileşeni olan keratin ile modifiye edilmiştir. Keratin kaynağı olarak insan saçları kullanılmış ve Shindai özütlemesi ile keratin elde edilmiştir. Keratin çözeltisi BS membranlara emdirilerek malzemenin karakterizasyonu FTIR (Fourier dönüşümlü kızıl ötesi spektrometresi), SEM (Taramalı elektron mikroskobu) ve mekanik çekme dayanımı testleri ile gerçekleştirilmiştir. Sonuç olarak, arttırılmış gözenek çapına sahip (>100μm) yapısında keratin bulunduran ve 0,1- 0,15 MPa aralığında maksimum çekme dayanımı gösteren, deri ikame adayı olabilecek BS üretimi gerçekleştirilmiştir.

Anahtar Kelimeler

• Bakteriyel selüloz
• keratin
• deri
• biyokompozit malzemeler

Destekleyen Kurum

Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Proje Numarası

FYL-2018-20231

Teşekkür

Bu çalışma Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından FYL-2018-20231nolu proje ile desteklenmiştir.

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