Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri

Elif Mutlu; Hasan H.s. Abuıyada

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Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri

Öz

Organotipik beyin kesit kültürleri günümüzde nörobiyoloji araştırmalarında rutin bir protokol haline gelmiştir. Beyin kesit kültürü tekniklerindeki ilerleme sayesinde birçok beyin hastalığı patofizyolojisinin in vivo duruma çok yakın bir şekilde doku bağlamında incelenmesi fırsatı doğmuştur. In vivo çalışmalarda anesteziklerin ve kas gevşetici maddelerin uzun süreli kullanımına alternatif olması yanında hücre kültürlerinin ve homojenatlarının aksine yapısal bütünlüğünü sürdürebilmesi gibi avantajlar sunarken bu kültür ortamı bazı dezavantajları da beraberinde getirmektedir. Bu derleme çalışması boyunca beyin kesit kültürü teknolojisi avantajları ve dezavantajları ile birlikte ele alınarak nörobiyoloji çalışmalarındaki yeri incelenmiştir. Hücresel bütünlüğün korunduğu bu sistemlerin nörodejenerasyon, nörogenez, nörotoksisite gibi birçok alanda in vitro hücre kültürü ve in vivo deney hayvanı çalışmalarına kıyasla çok daha verimli ve kullanışlıdır.

Anahtar Kelimeler

Beyin, Kesit kültürü, Organotipik beyin kesiti, Nörobiyoloji

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Yapısal Biyoloji

Bölüm

Derleme

Yazarlar

Elif Mutlu ^*
0000-0002-2580-4043
Türkiye

Hasan H.s. Abuıyada
0000-0002-5525-779X
Türkiye

Yayımlanma Tarihi

10 Haziran 2021

Gönderilme Tarihi

24 Mart 2021

Kabul Tarihi

22 Nisan 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 10 Sayı: 1

IZ

https://izlik.org/JA65ZN84JW

APA

Mutlu, E., & Abuıyada, H. H. (2021). Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(1), 95-107. https://izlik.org/JA65ZN84JW

AMA

1.Mutlu E, Abuıyada HH. Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri. DÜFED. 2021;10(1):95-107. https://izlik.org/JA65ZN84JW

Chicago

Mutlu, Elif, ve Hasan H.s. Abuıyada. 2021. “Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri”. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi 10 (1): 95-107. https://izlik.org/JA65ZN84JW.

EndNote

Mutlu E, Abuıyada HH (01 Haziran 2021) Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi 10 1 95–107.

IEEE

[1]E. Mutlu ve H. H. Abuıyada, “Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri”, DÜFED, c. 10, sy 1, ss. 95–107, Haz. 2021, [çevrimiçi]. Erişim adresi: https://izlik.org/JA65ZN84JW

ISNAD

Mutlu, Elif - Abuıyada, Hasan H.s. “Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri”. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi 10/1 (01 Haziran 2021): 95-107. https://izlik.org/JA65ZN84JW.

JAMA

1.Mutlu E, Abuıyada HH. Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri. DÜFED. 2021;10:95–107.

MLA

Mutlu, Elif, ve Hasan H.s. Abuıyada. “Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri”. Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c. 10, sy 1, Haziran 2021, ss. 95-107, https://izlik.org/JA65ZN84JW.

Vancouver

1.Elif Mutlu, Hasan H.s. Abuıyada. Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri. DÜFED [Internet]. 01 Haziran 2021;10(1):95-107. Erişim adresi: https://izlik.org/JA65ZN84JW

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https://doi.org/10.55007/dufed.xxxxxxx

Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri

Organotipik Beyin Kesitleri Kullanımının Nörobiyolojik Çalışmalardaki Yeri

Öz

Anahtar Kelimeler

Use of Organotypic Brain Slices in Neurobiological Studies

Öz

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Kaynakça

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Birincil Dil

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Yayımlandığı Sayı

IZ

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