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Öz

This research was conducted to determine the effects of green tea (Camellia sinensis L.) Seeds powder as a trout feed supplement on the immune response and disease resistance against Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). For this purpose, the fish were fed with tea (Camellia sinensis L.) seeds powder supplemented diet. Three different fish groups (negative control, positive control and trial group) were formed, and each group was two replicated. Rainbow trout (Oncorhynchus mykiss) fingerling (10 ±0,12g) obtains from research center were placed tanks (10 fish in each tank). Negative control group was established with fish normal diets were fed and not given the bacteria. For the positive control group were used in rainbow trout (Oncorhynchus mykiss) fingerling normal diets were fed and given the bacteria. Trial group was established with rainbow trout (Oncorhynchus mykiss) fingerling were fed with green tea (Camellia sinensis L.) seeds powder supplement diet and given the bacteria. Because of Aeromonas hydrophila symptoms of disease weren’t seen in trout (Oncorhynchus mykiss) fingerlings which were fed with green tea (Camellia sinensis L.) seeds powder supplement diet during ten days. While mortality was not seen innegative control group, was seen in positive control group as 40%. Results from this study confirmed the potential use of green tea (Camellia sinensis l.) seeds as a 10% feed additive for prevention of disease against Aeromonas hydrophila infection in rainbow trout (Oncorhynchus mykiss) fingerling culture

Anahtar Kelimeler

• Green tea seed, Aeromonas hydrophila, Immunostimulant

Çay Bitkisi (Camellia sinensis) Tohumunun Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Aeromonas hydrophila Enfeksiyonuna Karşı Kullanımının Araştırılması

Öz

Bu çalışmada, kültürü yapılan gökkuşağı alabalığının (Oncorhynchus mykiss) fingerling boydaki bireyleri üzerinde Aeromonas hydrophila’nın patojenik etkilerinin ortadan kaldırılması amaçlanmıştır. Bu amaç doğrultusunda alabalık yemine çay bitkisi (Camellia sinensis) tohumu ilave edilmiş ve hastalık etkeni olan Aeromonas hydrophila bakterisi yavrulara enfekte edilerek sonuçları gözlenmiştir.

Çalışmada öncelikle çay tohumu toz haline getirilmiş ve yemlere ilave edilmiştir. Araştırma merkezinden temin edilen fingerling boydaki (10 ±0,12) alabalıklar 10’lu gruplar halinde 6 deneme tankına yerleştirmiştir. Negatif kontrol olarak normal yemle yemlenmiş ve bakteri verilmemiş bir grup oluşturulmuştur. Pozitif kontrol için normal yem ile beslenen ve bakteri enfekte edilen balıklar kullanılmış, deneme için ise katkılı yemlerle beslenen balıklar ve bakteri enfekte edilen balıklar seçilmiştir. 10 günlük yemlemeden sonra yapılan gözlemlerde yeşil çay tohumu verilen gruplarda Aeromonas hydrophila bakterisinin neden olduğu hastalık belirtileri görülmemiştir. Negatif kontrol grubunda ölüm gözlemlenmezken, pozitif kontrol grubunda %40 oranında mortalite gözlemlenmiştir.

Sonuç olarak; elde edilen verilere göre; yeşil çay tohumunun Aeromonas hydrophila bakterisinin meydana getirdiği patojenik etkileri engellediği gözlemlenmiştir

Anahtar Kelimeler

• Çay tohumu, Aeromonas hydrophila, İmmunostimulant

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