Dumansız tütünde (Maraş otu) nikotinin elektrosprey iyonizasyon sıvı kromatografisi-tandem kütle spektrometrisi ile kantitatif tayini

Yıl 2021, , 1462 - 1471, 30.09.2021

Rukiye Aydın

https://doi.org/10.31202/ecjse.947874

Öz

Dumansız tütünde (Maraş otu) nikotin tayini için sıvı kromatografi-elektrosprey iyonizasyon tandem kütle spektrometresi kullanılarak hassas, hızlı, düşük maliyetli ve basit bir tayin yöntemi geliştirildi. Geliştirilen yöntem ile Maraş otu ve küllü Maraş otundaki nikotin miktarı belirlendi ve doğrulandı. Kromatografik ayırma, bir Hypersil Gold kolonu ile (2.1 mmx200 mm, 1.9 um) 30°C'de %0.2 asetik asitli su-asetonitril hareketli fazında 0.3 mLdak-1 akış hızında gerçekleştirildi. Toplam analiz süresi 10 dakikadır. Tandem kütle spektrometrik tayin SRM modunda pozitif elektrosprey iyonizasyon kullanılarak yapıldı. Yöntem spesifik ve duyarlı olup, LOD 0.057 mgL-1 ve LOQ 0.191 mgL-1 olarak hesaplandı. Doğrusallık, nikotin için 1 ila 10 mgL-1 arasında değişmiştir (R2=0.999, n=6). Yöntem Maraş otu örneklerine başarıyla uygulandı.

Anahtar Kelimeler

Nikotin, dumansız tütün, maraş otu, sıvı kromatografi-kütle spektrometrisi, elektrosprey iyonizasyon

Quantitative determination of nicotine in smokeless tobacco (Maras powder) by liquid chromatography-tandem mass spectrometry with electrospray ionization

Öz

A sensitive, fast, low-cost and simple determination method was developed for the determination of nicotine in smokeless tobacco (Maras powder) using liquid chromatography-electrospray ionization tandem mass spectrometry. With the developed method, the amount of nicotine in Maras powder and ashy Maras powder was determined and confirmed. Chromatographic separation was performed on a Hypersil Gold (2.1 mmx200 mm, 1.9 um) at 30 ◦C with a gradient of 0.2% acetic acid in water and acetonitrile at a flow rate of 0.3 mLmin-1; total run time was 10 min. Tandem mass spectrometric detection was used in positive electrospray ionization in SRM mode. The method was specific and sensitive; LOD was 0.057 mgL-1 and LOQ was 0.191 mgL-1; linearity ranged from 1 to 10 mgL-1 for nicotine (R2=0.999, n=6). The method was successfully applied to Maras powder samples.

Anahtar Kelimeler

Nicotine, smokeless tobacco, maras powder, liquid chromatography-mass spectrometry, electrospray ionization

Kaynakça

• [1]. Can G., Topbas M., Oztuna F., Ozgun S., Can E. Yavuzyılmaz A., ‘‘Factors contributing to regular smoking in adolescents in Turkey’’. Journal of school health, 2009, 79 (3): 93–97.
• [2]. Erenmemisoglu A., ‘‘Re Turkish smokeless tobacco "Maras Powder"’’, Prev Med, 1999, 28 616.
• [3]. Özkul Y., Donmez H., Erenmemisoglu A., Demirtas H., Imamoglu N., ‘‘Induction of micronuclei by smokeless tobacco on buccal mucosa cells of habitual users, Mutagenesis’’, 1997, 12 (4): 285–287
• [4]. Erenmemisoglu A., ‘‘The use of a smokeless tobacco in our country" Maras powder"’’, In 2nd International symposium of pharmaceutical sciences, 1999, 11-14 June Ankara-Turkey.
• [5]. Aral M., Ekerbicer H., Celik M., ‘‘Comparison of effects of smoking and smokeless tobacco “Maras powder” use on humoral immune system parameters’’, Mediators Inflamm, 2006, 3: 85019
• [6]. Erenmemisoglu A., Tekol Y., Kartal M., Kurucu S., ‘‘The use of a smokeless tobacco in our country "Maras Otu", 2nd International symposium of pharmaceutical sciences, 1991, 11-14 June, Ankara-Turkey
• [7]. Erenmemisoglu A., Tekol Y., Kartal M., Kurucu S., ‘‘Yurdumuzda dumansız bir tütün kullanımı ‘‘Maras Otu’’, Doga-Turk J Med Sci, 1992,16 567-76
• [8]. Nicotiana rustica", Natural Resources Conservation Service PLANTS Database, USDA, 2015, Retrieved 20 July
• [9]. BSBI List 2007, Botanical Society of Britain and Ireland. Archived from the original (xls) on 2014-10-23, 2014: 10-17
• [10]. Stephen B. S., André L. O. da S., Joseph G. L., Tameka S. L., Peter K. et al, ‘‘Comprehensive chemical characterization of Rapé tobacco products: Nicotine, un-ionized nicotine, tobacco-specific N′-nitrosamines, polycyclic aromatic hydrocarbons, and flavor constituents’’, Food and Chemical Toxicology, 2015, 8:50-58
• [11]. Katherine M.R.,"Nicotiana sp", artsci.wustl.edu., Archived from the original on 2008, 09-06
• [12]. Christen A.G., ‘‘The impact of tobacco use and cessation on oral and dental diseases and conditions’’, The American journal of medicine, 1992,93 (1A): 25-31
• [13]. Accortt N. A., Waterbor J. W., Beall C., Howard G., ‘‘Chronic disease mortality in a cohort of smokeless tobacco users’’, American Journal of Epidemiology, 2002, 156 (8):730–737
• [14]. Güven A., Köksal N., Büyükbese M. A., Cetinkaya A., Sökmen G. et al., ‘‘Effects of using a different kind of smokeless tobacco on cardiac parameters: “MP”, Anatolian J Cardiol, 2003, 3: 230–5
• [15]. Mitchell B.E., Sobel H. L., Alexander M.H., ‘‘The adverse health effects of tobacco and tobacco-related products Primary Care’’, Clinics in Office Practice, 1999, 26 (3), 463–498
• [16]. Tabak L., ‘‘Is smokeless tobacco less harmful than smokingv, Anadolu kardiyoloji dergisi, 2003, 3 (3), 236-237
• [17]. Sardas S., Cimen B., Karsli S., Yurdun T., Donbak L., ‘‘Comparison of genotoxic effect between smokeless tobacco (Maras powder) users and cigarette smokers by the alkaline comet assayv, Human&experimental toxicology, 2009, 28 (4), 214–219
• [18]. Kilinc M., Okur E., Kurutas E. B., Guler F. I., Yildirim I., ‘‘The effects of Maras powder (smokeless tobacco) on oxidative stress in users’’, Cell biochemistry and function Cell Biochem Funct., 2004, 22, 233–236
• [19]. Cok I., Ozturk R., ‘‘Urinary cotinine levels of smokeless tobacco (Maras powder) users’’, Human&experimental toxicology, 2000, 19 (11): 650–655
• [20]. Goud S. N., Zhang L., Kaplan A. M., ‘‘Immunostimulatory potential of smokeless tobacco extract in in vitro cultures of murine lymphoid tissues’’, Immunopharmacology, 1993, 25 (2):95-105
• [21]. Güven A., Tolun F., ‘‘Effects of smokeless tobacco “Maras Powder” use on nitric oxide and cardiovascular risk parameters’’, International journal of medical sciences, 2012, 9 (9), 786
• [22]. Kılınç M., Okur E., Yıldırım İ., İnanç F., Belge E. K., The investigation of the effect of Maras powder (smokeless tobacco) on hematological parameters’’, Turkish Society of Haematology, 2004, 21(3): 131-136
• [23]. Kilinc M., Okur E., Kurutas E. B., Güler F.I., Yıldırım I., ‘‘The effects of Maras powder (smokeless tobacco) on oxidative stress in users’’, Cell Biochem Funct, 2004, 22 (4), 233-6
• [24]. Güven A., Köksal N., Büyükbeşe M. A., Cetinkaya A., Sökmen G. et al., ‘‘Effects of using a different kind of smokeless tobacco on cardiac parameters: “Maras Powder’’, Anadolu Kardiyol. Derg, 2003,3(3): 230-235
• [25]. Buyukbeşe M. A., Koksal N., Guven A., Cetinkaya A., ‘‘Effects of smokeless tobacco “Maras powder” use on respiratory functions’’, The Tohoku journal of experimental medicine, 2004, 204 (3), 173–178
• [26]. Köksal N., Güven A., Çetinkaya A., Büyükbeşe M.A., ‘‘Dumansız Tütün “Maraş Otu” Kullanımının Solunum Fonksiyonları Üzerine Olan Etkileri’’, Akciğer Arşivi, 2004, 5(4): 174-8
• [27]. Keten H. S., Ölmez S., Üçer H., Işık O., Yıldırım F., Çelik M., ‘‘Acute effects of Maras powder (smokeless tobacco) on blood pressure and heart rate’’, Cukurova Med J, 2017, 42(2):210-215
• [28]. Köksal N., İnanç F., Kılınç M., ‘‘Sigara ve dumansız tütün (Maraş otu) kullananlarda serum adenozin deaminaz düzeyleri’’, Tıp Araştırmaları Dergisi, 2004, 2(3): 7-11
• [29]. Ozgur E., Keten H. S., Satan Y., Olmez S., Ucer H. et al., ‘‘Determınatıon Of The Relatıonshıp Between Cıgarette And Smokeless Tobacco (Maras Powder) Use and Depressıon/Anxıety’’, Acta Medica Mediterranea, 2015, 31(4): 899-905
• [30]. Vlase L., Filip L., Mîndruţău I., Sorin E.L., ‘‘Determınatıon Of Nıcotıne From Tobacco By LC-MS/MS’’, Studıa Unıversıtatıs Babeşbolyaı, Physıca, L, 2005, 4b