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First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium

Yıl 2018, Cilt 14, Sayı 3, 166 - 172, 01.09.2018
https://doi.org/10.22392/egirdir.371581

Öz

In this study, moribund salema (Sarpa salpa) individuals from a public aquarium were investigated to identify the cause of disease. A minor scale loss and partial skin depigmentation were observed in the diseased fish. Internal investigation of fish revealed hyperemia in the liver and the intestines and haemorrhages in the muscle tissues. When the histopathological sections of tissues were examined, it was determined that the bacterial infection affected especially the gill epithelium, the intestinal mucosa, the heart muscle and hemopoietic tissues of the spleen. According to phenotypic and biochemical characteristics as well as SSU rRNA sequencing results, the isolated bacteria was Vibrio harveyi and consistently seen all the examined samples of visceral organs including kidney, spleen, liver and blood. However, we need to emphasize that Vibrio vulnificus and Pseudoalteromonas piscicida bacteria were also isolated from blood and spleen tissues of one sick fish among others.

Kaynakça

  • Austin, B., & Zhang, X. H. (2006). Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Letters in Applied Microbiology, 43(2), 119-124. doi:10.1111/j.1472-765X.2006.01989.x
  • Balebona, M. C., Zorrilla, I., Moriñigo, M. A., & Borrego, J. J. (1998). Survey of bacterial pathologies affecting farmed gilt-head sea bream (Sparus aurata L.) in southwestern Spain from 1990 to 1996. Aquaculture, 166(1), 19-35. doi:10.1016/S0044-8486(98)00282-8
  • Clarridge, J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology Reviews, 17(4), 840-862. doi:10.1128/CMR.17.4.840-862.2004
  • Company, R., Sitj, A., Pujalte, M., Garay, E., & Alvarez‐Pellitero, P. (1999). Bacterial and parasitic pathogens in cultured common dentex, Dentex dentex L. Journal of fish diseases, 22(4), 299-309. doi:10.1046/j.1365-2761.1999.00182.x
  • Culling, C. F. A. (1963). Handbook of histopathological techniques (including museum technique).
  • Gusset, M., & Dick, G. (2011). The global reach of zoos and aquariums in visitor numbers and conservation expenditures. Zoo Biology, 30(5), 566-569. doi:10.1002/zoo.20369
  • Haldar, S., Maharajan, A., Chatterjee, S., Hunter, S., Chowdhury, N., Hinenoya, A., . . . Yamasaki, S. (2010). Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta. Microbiological research, 165(8), 639-648. doi:10.1016/j.micres.2009.12.001
  • Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Paper presented at the Nucleic Acids Symposium Series.
  • Larkin, M. A., Blackshields, G., Brown, N., Chenna, R., McGettigan, P. A., McWilliam, H., . . . Lopez, R. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947-2948.
  • López, J. R., de la Roca, E., Núñez, S., de la Herran, R., Navas, J. I., Manchado, M., . . . Toranzo, A. E. (2009). Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata. Diseases of Aquatic Organisms, 84, 209-217. doi:10.3354/dao02045
  • Pujalte, M., Sitja-Bobadilla, A., Macián, M., Belloch, C., Alvarez-Pellitero, P., Perez-Sanchez, J., . . . Garay, E. (2003). Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass. Systematic and applied microbiology, 26(2), 284-292. doi:10.1078/072320203322346146
  • Qin, Y., Wang, J., Su, Y., Wang, D., & Chen, X. (2006). Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara. Acta Oceanologica Sinica, 25(1).
  • Russell, B., Pollard, D., Mann, B.Q., Buxton, C.D. & Carpenter, K.E. (2014). Sarpa salpa. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2014-3.RLTS.T170169A1286510.en.
  • Saeed, M. (1995). Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait. Aquaculture, 136(1), 21-29. doi:10.1016/0044-8486(95)01045-9
  • Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D., & Doré, J. (1999). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Applied and environmental microbiology, 65(11), 4799-4807.
  • Tlusty, M. F., Rhyne, A. L., Kaufman, L., Hutchins, M., Reid, G. M., Andrews, C., . . . Dowd, S. (2013). Opportunities for public aquariums to increase the sustainability of the aquatic animal trade. Zoo Biology, 32(1), 1-12. doi:10.1002/zoo.21019
  • Turgay, E., & Karataş, S. (2016). First Report of Vibrio harveyi Infection in Diseased Common Dentex (Dentex dentex) Cultured in Turkey. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 12(2), 170-176.
  • Whitman, K. A. (2004). Finfish and Shellfish Bacteriology Manual: Techniques and Procedures: Iowa state press.
  • Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computational biology, 7(1-2), 203-214. doi:10.1089/10665270050081478
  • Zorrilla, I., Arijo, S., Chabrillon, M., Diaz, P., Martinez‐Manzanares, E., Balebona, M., & Morinigo, M. (2003). Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products. Journal of fish diseases, 26(2), 103-108. doi:10.1046/j.1365-2761.2003.00437.x

Bir Deniz Akvaryumunda Salpa Balıklarında (Sarpa salpa) Vibrio harveyi Enfeksiyonu Olgusu: ilk rapor

Yıl 2018, Cilt 14, Sayı 3, 166 - 172, 01.09.2018
https://doi.org/10.22392/egirdir.371581

Öz

Bu çalışma, bir deniz akvaryumundan alınan hasta salpa balıklarında (Sarpa salpa) hastalığa neden olan etken veya etkenleri tanımlamak üzere yapılmıştır. Hasta balıkların vücudunda hafif şiddette deri renginde açılma ile birlikte pul kayıpları gözlenmiştir. İç bakıda, karaciğer ve bağırsak dokularının hiperemik ve kas dokusunun da hemorajik olduğu tespit edilmiştir. Doku kesitleri histopatolojik olarak incelendiğinde, enfeksiyonun; özellikle solungaç epitelyumu, bağırsak mukozası, kalp kası ve dalağın hematopoetik dokusunu etkilediği belirlenmiştir. Fenotipik ve biyokimyasal özellikleri ve SSU rRNA dizileme sonuçlarına göre; incelenen tüm iç organlardan (böbrek, dalak, karaciğer) ve kan dokusundan Vibrio harveyi bakterisi izole edilmiştir. Buna ek olarak, incelenen balıklardan sadece birinin kan dokusundan ve dalağından alınan örneklerde Vibrio vulnificus ve Pseudoalteromonas piscicida bakterileri de izole edilmiştir.

Kaynakça

  • Austin, B., & Zhang, X. H. (2006). Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Letters in Applied Microbiology, 43(2), 119-124. doi:10.1111/j.1472-765X.2006.01989.x
  • Balebona, M. C., Zorrilla, I., Moriñigo, M. A., & Borrego, J. J. (1998). Survey of bacterial pathologies affecting farmed gilt-head sea bream (Sparus aurata L.) in southwestern Spain from 1990 to 1996. Aquaculture, 166(1), 19-35. doi:10.1016/S0044-8486(98)00282-8
  • Clarridge, J. E. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clinical Microbiology Reviews, 17(4), 840-862. doi:10.1128/CMR.17.4.840-862.2004
  • Company, R., Sitj, A., Pujalte, M., Garay, E., & Alvarez‐Pellitero, P. (1999). Bacterial and parasitic pathogens in cultured common dentex, Dentex dentex L. Journal of fish diseases, 22(4), 299-309. doi:10.1046/j.1365-2761.1999.00182.x
  • Culling, C. F. A. (1963). Handbook of histopathological techniques (including museum technique).
  • Gusset, M., & Dick, G. (2011). The global reach of zoos and aquariums in visitor numbers and conservation expenditures. Zoo Biology, 30(5), 566-569. doi:10.1002/zoo.20369
  • Haldar, S., Maharajan, A., Chatterjee, S., Hunter, S., Chowdhury, N., Hinenoya, A., . . . Yamasaki, S. (2010). Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta. Microbiological research, 165(8), 639-648. doi:10.1016/j.micres.2009.12.001
  • Hall, T. A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Paper presented at the Nucleic Acids Symposium Series.
  • Larkin, M. A., Blackshields, G., Brown, N., Chenna, R., McGettigan, P. A., McWilliam, H., . . . Lopez, R. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947-2948.
  • López, J. R., de la Roca, E., Núñez, S., de la Herran, R., Navas, J. I., Manchado, M., . . . Toranzo, A. E. (2009). Identification of Vibrio harveyi isolated from diseased cultured wedge sole Dicologoglossa cuneata. Diseases of Aquatic Organisms, 84, 209-217. doi:10.3354/dao02045
  • Pujalte, M., Sitja-Bobadilla, A., Macián, M., Belloch, C., Alvarez-Pellitero, P., Perez-Sanchez, J., . . . Garay, E. (2003). Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass. Systematic and applied microbiology, 26(2), 284-292. doi:10.1078/072320203322346146
  • Qin, Y., Wang, J., Su, Y., Wang, D., & Chen, X. (2006). Studies on the pathogenic bacterium of ulcer disease in Epinephelus awoara. Acta Oceanologica Sinica, 25(1).
  • Russell, B., Pollard, D., Mann, B.Q., Buxton, C.D. & Carpenter, K.E. (2014). Sarpa salpa. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2014-3.RLTS.T170169A1286510.en.
  • Saeed, M. (1995). Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait. Aquaculture, 136(1), 21-29. doi:10.1016/0044-8486(95)01045-9
  • Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D., & Doré, J. (1999). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Applied and environmental microbiology, 65(11), 4799-4807.
  • Tlusty, M. F., Rhyne, A. L., Kaufman, L., Hutchins, M., Reid, G. M., Andrews, C., . . . Dowd, S. (2013). Opportunities for public aquariums to increase the sustainability of the aquatic animal trade. Zoo Biology, 32(1), 1-12. doi:10.1002/zoo.21019
  • Turgay, E., & Karataş, S. (2016). First Report of Vibrio harveyi Infection in Diseased Common Dentex (Dentex dentex) Cultured in Turkey. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 12(2), 170-176.
  • Whitman, K. A. (2004). Finfish and Shellfish Bacteriology Manual: Techniques and Procedures: Iowa state press.
  • Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of Computational biology, 7(1-2), 203-214. doi:10.1089/10665270050081478
  • Zorrilla, I., Arijo, S., Chabrillon, M., Diaz, P., Martinez‐Manzanares, E., Balebona, M., & Morinigo, M. (2003). Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products. Journal of fish diseases, 26(2), 103-108. doi:10.1046/j.1365-2761.2003.00437.x

Ayrıntılar

Birincil Dil İngilizce
Konular Deniz ve Tatlı Su Biyolojisi
Bölüm Makaleler
Yazarlar

Emre TURGAY> (Sorumlu Yazar)
Istanbul University, Faculty of Aquatic Sciences, Department of Fish Diseases
Türkiye


Remziye Eda YARDIMCI>
Istanbul University, Faculty of Aquatic Sciences, Department of Fish Diseases
0000-0001-7737-8739
Türkiye


Süheyla KARATAŞ>
Istanbul University, Faculty of Aquatic Sciences, Department of Fish Diseases
Türkiye

Yayımlanma Tarihi 1 Eylül 2018
Yayınlandığı Sayı Yıl 2018, Cilt 14, Sayı 3

Kaynak Göster

Bibtex @diğer { egirdir371581, journal = {Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi}, eissn = {1308-7517}, address = {Ankara Üniversitesi Eczacılık Fakültesi Farmakognozi ABD 06100 Tandogan-Ankara}, publisher = {Isparta Uygulamalı Bilimler Üniversitesi}, year = {2018}, volume = {14}, number = {3}, pages = {166 - 172}, doi = {10.22392/egirdir.371581}, title = {First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium}, key = {cite}, author = {Turgay, Emre and Yardımcı, Remziye Eda and Karataş, Süheyla} }
APA Turgay, E. , Yardımcı, R. E. & Karataş, S. (2018). First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium . Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi , 14 (3) , 166-172 . DOI: 10.22392/egirdir.371581
MLA Turgay, E. , Yardımcı, R. E. , Karataş, S. "First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium" . Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi 14 (2018 ): 166-172 <https://dergipark.org.tr/tr/pub/egirdir/issue/36732/371581>
Chicago Turgay, E. , Yardımcı, R. E. , Karataş, S. "First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium". Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi 14 (2018 ): 166-172
RIS TY - JOUR T1 - Bir Deniz Akvaryumunda Salpa Balıklarında (Sarpa salpa) Vibrio harveyi Enfeksiyonu Olgusu: ilk rapor AU - EmreTurgay, Remziye EdaYardımcı, SüheylaKarataş Y1 - 2018 PY - 2018 N1 - doi: 10.22392/egirdir.371581 DO - 10.22392/egirdir.371581 T2 - Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi JF - Journal JO - JOR SP - 166 EP - 172 VL - 14 IS - 3 SN - -1308-7517 M3 - doi: 10.22392/egirdir.371581 UR - https://doi.org/10.22392/egirdir.371581 Y2 - 2018 ER -
EndNote %0 Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium %A Emre Turgay , Remziye Eda Yardımcı , Süheyla Karataş %T First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium %D 2018 %J Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi %P -1308-7517 %V 14 %N 3 %R doi: 10.22392/egirdir.371581 %U 10.22392/egirdir.371581
ISNAD Turgay, Emre , Yardımcı, Remziye Eda , Karataş, Süheyla . "First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium". Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi 14 / 3 (Eylül 2018): 166-172 . https://doi.org/10.22392/egirdir.371581
AMA Turgay E. , Yardımcı R. E. , Karataş S. First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium. SDU-JEFF. 2018; 14(3): 166-172.
Vancouver Turgay E. , Yardımcı R. E. , Karataş S. First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi. 2018; 14(3): 166-172.
IEEE E. Turgay , R. E. Yardımcı ve S. Karataş , "First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium", Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, c. 14, sayı. 3, ss. 166-172, Eyl. 2018, doi:10.22392/egirdir.371581