A Study of Preparation and Characterization of Crystal Violet Incorporated Polyacrylamide Gel (PAG) Dosimeters

Öz

Biomaterials and medical products intended for human contact undergo sterilization according to international standards. In biomedical applications, gamma radiation is the preferred sterilization method due to its superior properties. The applied dose of gamma radiation must be thoroughly validated to ensure patient safety and mitigate potential risks. Polymer gel dosimeters confirm whether a specified gamma sterilization dose is administered. In this study, a novel type of polymer gel dosimeter was developed, based on acrylamide with the addition of crystal violet dye. The doseimeters were irradiated with a 60Co gamma source at doses ranging from 7-28 kGy and at lower doses (30-90 mGy) for biomedical sterilization. FT-IR analyses were conducted for the purpose of structural characterization. Absorbance measurements were taken before and after irradiation using a a UV-Vis spectrophotometer to determine the dosimeter's dose response. Crystal violet (CV)-doped polyacrylamide gel (PAG) dosimeters have exhibited linear sensitivity to radiation dose. As the radiation dose increased, the dye's absorbance decreased, resulting in a lighter coloration of the solution. The stability of irradiated and non-irradiated dosimeters was measured at both room temperature and 4°C storage conditions over a 90-day period. The results indicated that the dosimeter exhibited stability under both environmental conditions. PAG dosimeters combined with CV additives show promise in validating dosing for sterilizing biomaterials and medical devices.

Anahtar Kelimeler

• Dosimetry
• Crystal violet
• Gamma radiation
• PAG dosimetry
• Sterilization

Kristal Viyole İçerikli Poliakrilamid Jel Dozimetrelerin Hazırlanması ve Karakterizasyonunun İncelenmesi

Öz

İnsan vücuduyla temasta bulunacak biyomalzeme ve tıbbi ürünlerin kullanılmadan önce steril edilmesi uluslararası standartlarla belirlenmiştir. Biyomedikal uygulamalarda gama radyasyonla sterilizasyon üstün özellikleri sebebiyle tercih edilmektedir. Hasta güvenliği ve uygulamalardaki risklerin azaltılması için uygulanan gama radyasyon dozu valide edilmelidir. Polimer jel dozimetreler, belirtilen bir gama sterilizasyon dozunun uygulanıp uygulanmadığını doğrulamak için kullanılır. Bu çalışmada, kristal viyole boyar maddesi katkılı akrilamid bazlı yeni tip polimer jel dozimetre geliştirilmiştir. Dozimetreler, 60Co gama kaynağı kullanılarak biyomedikal sterilizasyonda tipik olarak kullanılan 7-28 kGy doz aralığı ve düşük doz değerlerinde (30-90 mGy) ışınlanmıştır. Yapısal karakterizasyon için FT-IR analizleri yapılmıştır. Işınlama öncesi ve sonrası absorbans ölçümleri UV-Vis spektrofotometre ile gerçekleştirilerek dozimetrenin doz yanıtı belirlenmiştir. Kristal viyole (CV) katkılı poliakrilamid jel (PAG) dozimetreler, radyasyon dozuna lineer duyarlılık göstermiştir. Radyasyon dozu arttıkça boyar maddenin absorbansının azaldığı ve renginin açıldığı gözlemlenmiştir. Işınlanmış ve ışınlanmamış dozimetrelerin 90 gün süreyle oda sıcaklığı ve 4 °C saklama koşullarındaki kararlılığı stabil olarak ölçülmüştür. CV katkılı PAG dozimetrelerin biyomalzeme ve tıbbi cihazların sterilizasyonunda istenilen dozun valide edilmesinde kullanımı umut vadetmektedir.

Anahtar Kelimeler

• Dozimetre
• Kristal viyole
• Gama radyasyon
• PAG dozimetre
• Sterilizasyon

Kaynakça

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