Evaluation of antimalarial potential of aqueous stem bark extract of Bombax buonopozense P. Beauv. (Bombacaceae)

Yıl 2012, Cilt: 17 Sayı: 2, 72 - 77, 23.01.2013

Tom Chinyere Iwuanyanwu Godwin Christian Akuodor Augustine Dick Essien Florence Chidume Nwinyi Joseph Linus Akpan Dorcas Okayo Okorafor Uche Alex Osunkwo

https://izlik.org/JA69HE42DH

Öz

Abstract. The treatment of malaria in Africa is becoming increasingly difficult due to rising prevalence of plasmodium falciparum resistant to antimalarial drugs. This study was undertaken to investigate the in vivo antiplasmodial effect of the aqueous stem bark extract of Bombax buonopozense in mice infected with chloroquine-sensitive plasmodium berghei NK65. The in vivo antiplasmodial effect against early infection and curative effect against established infection were studied. The extract at all doses (100, 200 and 400 mg/kg, p.o.) used, exhibited significant (P<0.05), dose-dependent activity against the parasite in suppressive and curative tests. These results show that the stem bark extract of Bombax buonopozense plant has significant antiplasmodial effect. The reduction in parasitemia levels in the two employed models suggest that a part of its antimalarial activity is mediated by direct plasmocidal effect on the parasite.

Key words: Antimalaria, bombax buonopozense, medicinal plant, mice, plasmodium berghei

Anahtar Kelimeler

Antimalaria , bombax buonopozense , medicinal plant , mice , plasmodium berghei

Kaynakça

• Greenwood B and Mutabingwa. Malaria in 2002. Nature 2002; 415: 670-672.
• De Ridder S, VanderRoot f and Verpoore R. Artemisia annua as a self reliant treatment for malaria in developing countries. J. Ethnopharmacol 2008; 120: 302-314.
• Whitefield PJ. Plant allelocheicals and the control of parasites. Bull Scand Soc. Parasitol 1995; 5: 5- 18.
• Elujoba AA, Odeleye OM, ogunyemi CM. Traditional medicinal development for medical and dental primary health care delivery system in Africa. Afr. J. Trad. Complemet.Alternat. Med 2005; 2: 46-61.
• Beentj H, Sara S. Plant systemic and Phytogeography for the understanding of African Biodiversity. Syst. Geogr. Plant 2001; 71: 284-286.
• Akuodor GC, Mbah CC, Megwas UA, et al. In vivo antimalarial activity of methanol leaf extract of bombaxbuonopozense in mice Infected with plasmodium berghei. Intern. J. Biol. Chemical Sc 2011; 5: 1790-1796.
• Akuodor GC, Idris Usman M, Ibrahim JA, et al. Anti-nociceptive, anti-inflammatory and antipyretic effect of the methanolic extract of Bombax buonopozense leaves in rats and mice. Afr. J. Biotechnology 2011; 10: 3191- 3196.
• Dubost G. Comparism of the diets of frugivorous forest mammal of Gabon. J. Mammals 1984; 65: 298-316.
• Trease GE, Evans WC. Pharmacognosy 13th Ed. English Language Book Society, Bailliere, Tindal, London 1989; 176-180.
• Sofowora A. Medicinal Plants and traditional medicine in Africa. Spectrum Books, Ibadan. 1993; p.150.
• Lorke D. A new approach for acute practical toxicity testing. Arch. Toxicol 1983; 54: 273-287.
• ENV/MC/CHEM (98) 17. OECD series on principles of good laboratory practice and compliance monitoring number 1. OECD principles on good laboratory practice (as revised in 1997) Environmental Directorate Organization for Economic Co-operation and Development Paris. 1998. www.iri-phrma. Com/download/ principles on-GLP. Pdf.
• NIH publication. Respect for life. National Instititute of Environmental Health Sciences- NIEHS 1985; 85-23.
• Hilou A, Nacoulma OG, Guiguemde TR. In vivo antimalarial activities of extracts from Amaranthus spinosus l. and Boerhaavia erecta l. in mice. J. Ethnopharmacol 2006; 103: 236-240.
• Okokon JE, Ofodum KC, Ajibesin KK, Danladi B and Gamaniel KS. Pharmacological screening and evaluation of antiplasmodial activity of Croton zambescus against plasmodium berghei berghei infection in mice. Indian J. Pharmacol 2005; 37: 243-246.
• Akuodor GC, Idris-Usman M, Anyalewechi NA, et al. In vivo antimalarial activity of ethanolic leaf extract of Verbena hastata against plasmodium berghei berghei in mice. J. Herbal Med. And Toxicol 2010; 4: 117-123.
• Ryley JF and Peters W. The antimalarial activity of some quinolone esters. Ann. Trop. Med. Parasitol 1970; 84: 209-222.
• Chandel S and Bagai U. Antiplasmodial activity of Ajuga bracteosa against plasmodium bergei infected BALB/c mice. Indian J. Med. Res 2010; 131: 440- 444.
• Akuodor GC, Anyalewechi NA, Ikoro NC, et al. Evaluation of antiplasmodial activity of Berlina grandiflora leaf extract against plasmodium berggei in mice. Afr. J. Microbiol Res 2010; 4: 2211-2214.
• Betty K, Sternne J. Essential Medical Statistics Massachusetts. Black well science Ltd 2003; 2nd edition.
• Thomas AM, Van Derwel AM, Thomas AW, Jouse CJ, Waters AP. Transfection systems For animal models of malaria. Parasitol Today 1998; 14: 248- 249.
• Pedroni HC, Betton CC, Splalding SM, Coaster TD. Plasmodium: Development of Irreversible experimental malaria model in Wister rats. Exp. Parasitol 2006; 113: 193-196.
• English MC, Wuruiri C, Lightowler C. Murphy SA, Kirigha G, Marsh K. Hyponatreamia And dehydration in severe malaria. Arch. Dis. Childhood 1996; 74: 201-205.
• Kumar KA, Sign S, Babu PP. Studies on the glycoprotein modification in erythrocyte Membrane during experimental cerebral malaria. Exp. Parasitol 2006; 114: 173-179.
• Peter IT and Anatoli VK. The current global malarial situation. Malaria parasite biology. Pathogenesis and protection ASM Press W.D.C. 1998; pp 11-22.
• Ene AC, Ameh DA, Kwanashie HO, Agomuo PU, Atawodi SE. Preliminary in vivo Antimalarial screening of petroleum, chloroform and methanol extracts of fifteen plants grown in Nigeria. J. Pharmacol Toxicol 2008; 3: 254-260.
• Akuodor GC, Idris-Usman M, Ugwu TC, et al. In vivo Schizonticidal activity of ethanolic leaf extract of Gongronema latifolium on plasmodium berghei berghei in mice, Afr. J. Biotechnol 2010; 9: 2316- 2321.
• Steele JC, Simmonds MS, Veich NC and Warhurst DC. Evaluation of the antiplasmodium Activity of bisbenzisoquinoline alkaloids from Abuta grandifolia. Planta Med 1999; 5: 413-416.
• Aishawsh SM, Mothana RA, Al-shamahy HA, Alsllami SF and Lindequist U. Assessment of antimalarial activity against plasmodium falciparum and Phytochemical screening of some Yemeni medicinal plants. ECAM 2007; 6: 453-456.
• Matur BM, Mathew T and Ifeanyi CIC. Analysis of the phytochemical and in vivo antimalarial properties of Phyllanthus fratermus Webster extract. New York Sci J 2009; 2: 12-19.
• Philipson JD and Wright CW. Antiprotozoal compound from plant source. Planta Medica 1990; 57: 553-559.
• Ayoola GA, Coker HAB, Adesegun SA, et al. Phytochemical screening and antioxidant acivity of some selected medicinal plants used for malaria therapy in South western Nigeria. Trop. J Pharm. Res 2008; 7: 1019-1024.
• Arokiyaraj S, Martin S, Perinbam K, Arockanathan PM and Beatrice V. Free radical Scavenging activity and HPTLC finger print of Pterocarus santalinus L. an in vitro study. Indian J. Sci. Technol 2008; 1: 1- 3.