Determination and evaluation in terms of healthy nutrition of the pyridoxal, pyridoxine and pyridoxamine forms of vitamin B6 in animal-derived foods

Abstract

In many studies, vitamin B6 is given as the sum of the pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) forms. In a limited number of studies, PL, PN and PM forms of vitamin B6 in animal origin foods were reported. Since the bioavailability of PL, PN and PM forms of vitamin B6 are different; knowing the amounts of these forms in foods is important in terms of healthy nutrition. In this study, the PL, PN and PM forms in a total of 38 animal-based foods were determined by high performance liquid chromatography (HPLC). PL and PM were found predominantly in fish, meats and chicken samples. Within these, the highest amount of vitamin B6 were found in golden grey mullet by 616.3 µg/100g, in veal fillet at 376.1 µg/100g and in chicken breast by 329.5 µg /100g, respectively. The PL form in total vitamin B6 ranged in fish between 32.5 and 53.1%, in meats between 15.6 and 48.9%, and in chicken samples between 59.9 and 69.8%. It was also found that milk and milk products contain low amounts of vitamin B6. Based on these results, all animal-based foods were found to be rich in terms of the PL and PM forms. The results of this study will play an important role in the creation of various diets for healthy nutrition.

Keywords

• Vitamin B6 profile
• animal-based foods
• nutrition
• HPLC

Hayvansal kaynaklı gıdalardaki Vitamin B6’nın piridoksal, piridoksin ve piridoksamin formlarının belirlenmesi ve sağlıklı beslenmesi açısından değerlendirmesi

Öz

Birçok çalışmada vitamin B6 pridoksal, pridoksin ve pridoksamin formunun toplamı olarak verilmektedir. Sınırlı sayıdaki çalışmada vitamin B6’nın PL, PN ve PM formu raporlanmıştır. Vitamin B6’nın PL, PN ve PM formlarının biyoyararlanımı farklıdır; Besinlerde bu formların miktarlarının bilinmesi sağlıklı beslenme açısından önemlidir. Bu çalışmada, yüksek performanslı sıvı kromatografisi (HPLC) ile toplam 38 hayvansal kaynaklı gıdadaki vitamin B6’nın PL, PN ve PM formları belirlenmiştir. PL ve PM ağırlıklı olarak balıklarda, etlerde ve tavuk örneklerinde bulmuştur. Balıklarda, etlerde ve tavuk örneklerinde en yüksek miktarda B6 vitamini sırayla kefalde 616.3 µg/100g, dana filetoda 376.1 µg/100g ve tavuk göğsünde 329.5 µg/100g olarak bulundu. Toplam B6 vitaminindeki PL formu balıklarda% 32,5 ile% 53,1, etlerde% 15,6 ile% 48,9 ve tavuk örneklerinde% 59,9 ile% 69,8 arasında değişmiştir. Süt ve süt ürünlerinde düşük miktarda B6 vitamini içerdiği bulunmuştur. Bu sonuçlara bakıldığında, tüm hayvansal gıdaların PL ve PM formları açısından zengin olduğu tespit edilmiştir. Elde ettiğimiz bu sonuçlar sağlıklı beslenme açısından çeşitli diyetlerin oluşturulmasında önemli bir rol oynayacaktır.

Anahtar Kelimeler

• Vitamin B6
• hayvansal kaynaklı gıdalar
• beslenme
• HPLC

Kaynakça

1. Ball, G.F.M. (2004). Vitamin B6. Vitamins: Their Role in the Human Body. Blackwell Publishing Ltd, Oxford, UK. 310-325.
2. Bilski, P., Li, M.Y., Ehrenshaft, M., Daub, M.E. and Chignell, C.F. (2000(. Vitamin B6 (pyridoxine) and its derivatives are efficient singlet oxygen quenchers and potential fungal antioxidants. Photochemistry and Photobiology 71:129–34.
3. Bowers, J.A. and Craig, J. (1978). Components of vitamin B6 in turkey breast muscle. Journal of Food Science, 43(5):1619-1619.
4. Ceylan, Z., Yaman, M., Sağdıç, O. and Karabulut, E., Yilmaz, M.T. (2018). Effect of electrospun thymol-loaded nanofiber coating on vitamin B profile of gilthead sea bream fillets (Sparus aurata), LWT 98:162-169.
5. Drewke, C. and Leistner, E. (2001). Biosynthesis of vitamin B6 and structurally related derivatives. In vitamins and hormones (Litwack, G., ed.). Academic Press, San Diego, CA, 121–155.
6. DTU. (2018). National Food Institute - Technical University of Denmark (DTU), Danish Food Composition Databank). www.foodcomp.dk/fcdb_default.asp, Accessed December 21, 2018.
7. Esteve, M.J., Farre, R., Frıgola, A. and Garcia-Cantabella, J.M. (1998). Determination of vitamin B (pyridoxamine, pyridoxal and 6 pyridoxine) in pork meat and pork meat products by liquid chromatography. Journal of Chromatography A 795:383–387
8. [8] Food and Nutrition Board. (1998). Vitamin B6: Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline, National Academy Press, Washington, DC. 150–95.

9. Gratacós-Cubarsí, M., Sárraga, C., Castellari, M., Guàrdia, M.D., Regueiro, J.A. and Arnau J. (2013). Vitamin (B1, B2, B3 and B6) content and oxidative stability of Gastrocnemius muscle from dry-cured hams elaborated with different nitrifying salt contents and by two ageing times. Meat Science 95(1):647-651.[10] Gregory, J. F. III., Trumbo, P. R., Bailey, L. B., Toth, J. P., Baumgartner, T. G. And Cerda, J. J. (1991). Bioavailability of pyridoxine-5´-β-D glucoside determined in humans by stable-isotopic methods. Journal of Nutrition 121:177–86
10. Gregory, J.F.III. and Ink, S.L. (1987). Identification and quantification of pyridoxine-β-glucoside as a major form of vitamin B6 in plant-derived foods. Journal of Agricultural and Food Chemistry 35:76–82.
11. Hansen, C. M., Leklem, J. E. And Miller, L.T. 1996. Vitamin B-6 status of women with a constant intake of vitamin B-6 changes with three levels of dietary protein. Journal of Nutrition 126:1891–901.
12. [13] Jansen, M.C., Bueno-de-Mesquita, H.B., Buzina, R., Fidanza, F., Menotti, A., Blackburn, H., Nissinen, A.M., Kok, F.J.and Kromhout, D. (1999). Dietary fiber and plant foods in relation to colorectal cancer mortality: the seven countries study. International Journal of Cancer 81:174-179.
13. Kall, M. A. (2003). Determination of total vitamin B6 in foods by isocratic HPLC: a comparison with microbiological analysis. Food Chemistry 82(2):315-327.
14. Lebiedzińska, A.., Marcin, L.M. and Jadwiga, K.P.S. (2007). Reversed-phase high-performance liquid chromatography method with coulometric electrochemical and ultraviolet detection for the quantification of vitamins B1 (thiamine), B6 (pyridoxamine, pyridoxal and pyridoxine) and B12 in animal and plant foods. Journal of Chromatography A 1173(1–2):71-80.
15. Mann, D.L., Ware, G, M., Bonnin, E.and Eitenmiller, R.R. (2001). Liquid Chromatographic Analysis of Vitamin B6 in Reconstituted Infant Formula: Collaborative Study. The Journal of AOAC International 88(1):30-37(8).
16. Miller, J. W., Ribaya-Mercado, J. D., Russell, R. M., Shepard, D. C., Morrow, F. D., Cochary, E. F., Sadowski, J. A., Gershoff, S. N. and Selhub, J. (1992). Effect of vitamin B-6 defi ciency on fasting plasma homocysteine concentrations. American Journal of Clinical Nutrition 55:1154–60.
17. Miller, L.T., Leklem, J.E.and Shultz, T.D. (1985). The effect of dietary protein on the metabolism of vitamin B-6 in humans. Journal of Nutrition 115:1663–72.
18. [19] Mittenhuber, G. (2001). Phylogenetic analyses and comparative genomics of vitamin B6 (pyridoxine) and pyridoxal phosphate biosynthesis pathways. Journal of Molecular Microbiology and Biotechnology 3:1–20.
19. Mizushina, Y., Yoshida, S., Matsukage, A. and Sakaguchi, K. (1997). The inhibitory action of fatty acids on DNA polymerase β. Biochimica et Biophysica Acta 1336:509-521.
20. Nakamura, S. And Niwa, T. (2005). Pyridoxal phosphate and hepatocyte growth factor prevent dialysate-induced peritoneal damage. Journal of The American Society of Nephrology 16:44-150.
21. Olds, S.J., Vanderslice, J.T. and Brochetti, D. 1993. Vitamin B6 in Raw and Fried Chicken by HPLC. Journal of Food Science 58(3):505-507.Rossouw, J.E., Labadarios, D., Davis, M.and Williams, R. (1978).Vitamin B6 and aspartate aminotransferase activity in chronic liver disease. The South African Medical Journal, 53(12):436-8.
22. Sang, Y.R.and Young S.K. (2018.) The Role of Advanced Glycation End Products in Diabetic Vascular Complications. DiabetesandMetabolism 42(3):188–195.[24] Slattery, M.L., Potter, J.D., Coates, A., Ma, K.N., Berry, T.D., Ducan, D.M. and Caan, D.J. (1997). Plant foods and colon cancer: an assessment of specific foods and their related nutrients (United States). Cancer Causes Control 8:575-590.
23. Su-Yen, G. and Mark, E.C. (2008). The Role of Advanced Glycation End Products in Progression and Complications of Diabetes. The Journal of Clinical Endocrinology and Metabolism, 93(41):1143–1152.
24. TURKOMP. (2018). Turkish Food Composition Database, http://www.turkomp.gov.tr/main, Accessed December 21, 2018.
25. [USDA. (2018). United States Department of Agriculture. USDA Food Composition Databases. Available at: https://ndb.nal.usda.gov/ndb/, Accessed December 21, 2018.Wozenski, J.R., Leklem, J.E. and Miller, L.T. (1980). The metabolism of small doses of vitamin B-6 in men. Journal of Nutrition 110:275-285.