Assessment of Wild R. pimpinellifolia L. According to Mineral Content and Bioactive Compounds

Abstract

In this study the characteristics of the fruits of R. pimpinellifolia as a naturally grown and wild species in Bayburt was investigated. The antioxidant property of this fruit was determined by using four different methods; DPPH, ABTS, CUPRAC and β-carotene bleaching method in both water and methanolic extracts. Total phenolic content and phenolic profiles were determined by FCR (Folin-Ciocalteu reagent) method and HPLC, with respect. Additionally mineral contents and some physicochemical properties were analyzed. The antioxidant activities obtained from aqueous extract as 0.56 mmol TR/g-sample for CUPRAC, 97.02% for β-carotene bleaching method, 88.65% for ABTS and 86.69% for DPPH. For the methanol extraction antioxidant activities recorded as 0.99 mmol TR/g-sample for CUPRAC, 95.21% for β-carotene bleaching method, 93.43% for ABTS and 85.21% for DPPH. Total phenolic compound level was determined as 929.27 mg GAE/100 g in aqueous extract and that was found to be higher than methanolic extract. The most dominant phenolic compounds in the R. pimpinellifolia were detected as chlorogenic acid, vanillic acid and gallic acid. The dominant minerals found in this wild fruit were K (12.1688 mg/ml), P (1.3429 mg/ml) and Mg (1.1926 mg/ml) whereas Cr (0.00001 mg/ml) was minimum. Consequently, black rosehip (R. pimpinellifolia) fruit can be suggested to be very good natural nutrient source due to its high content of phenolic compounds and minerals and important antioxidant activity and phenolic.

Keywords

• R. pimpinellifolia
• Mineral content
• Antioxidant activity
• Phenolic profile
• Total phenolic compound

Yabani Kara Kuşburnu (R. pimpinellifolia L.) Meyvesinin Mineral İçerik ve Biyoaktif Bileşenler Açısından Değerlendirilmesi

Öz

Bu çalışmada Bayburt ilinde doğal olarak yetişen yabani R. pimpinellifolia L. meyvesinin genel özellikleri araştırılmıştır. Bu meyvenin antioksidan özellikleri dört farklı yöntem (DPPH, ABTS, CUPRAC ve β-karoten ağartma) kullanılarak hem su hem de metanol ekstrelerinde belirlenmiştir. Toplam fenolik madde içeriği ve fenolik profili sırası ile FCR (Folin-Ciocalteu ayıracı) metodu ve HPLC yardımıyla analiz edilmiştir. Bunlara ilaveten mineral madde içeriği ve bazı fizikokimyasal özellikleri ele alınmıştır. Su ekstresinde meyve antioksidan seviyeleri CUPRAC yönteminde 0.56 mmol TR/g-örnek, β-karoten ağartma yönteminde %97.02, ABTS metodunda %88.65 ve DPPH metodunda ise %86.69 olarak tespit edilmiştir. Metanol ekstresinde ise CUPRAC yönteminde 0.99 mmol TR/g-örnek, β-karoten yönteminde %95.21, ABTS metodunda %93.43 ve DPPH metodunda ise %85.21 olarak tespit edilmiştir. Toplam fenolik bileşen seviyesi ise sulu ekstresinde 929.27 mg GAE/100g olarak belirlenmiş olup bu değer metanol ekstresinde elde edilenden yüksektir. Meyvenin içerdiği fenolik bileşenler arasında en baskın olanların sırası ile klorojenik asit, vanilik asit ve gallik asit olduğu belirlenmiştir. Meyvede bulunan minerallerin arasında en yoğun olanlar sırası ile K (12.1688 mg/ml), P (1.3429 mg/ml) ve Mg (1.1926 mg/ml) olurken en az bulunan mineralin ise Cr (0.00001 mg/ml) olduğu açığa çıkartılmıştır. Sonuç olarak kara kuşburnu meyvesinin (R. pimpinellifolia) sahip olduğu bu özelliklerden dolayı çok iyi bir doğal besin olarak değerlendirilebileceği anlaşılmıştır.

Anahtar Kelimeler

• R. pimpinellifolia
• Mineral madde
• Antioksidan aktivite
• Total fenolik madde içeriği
• Fenolik profil

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