In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method with high separation efficiency, high detection sensitivity and excellent selectivity was successfully developed for the simultaneous quantitative determination of the aloe-emodin, rhein, emodin, chrysophanol, and physcion compounds. These optimum method conditions were determined by studying various columns, mobile phases and compositions, flow rates and column temperatures. The successful separation was carried out with a Supelcosil LC-18 column (250 × 4.6 mm, 5 µm) and a gradient program. The procedure was carried out at 20 °C with the flow rate of 1.0 mL/min and the injection volume of 20 μL utilizing an RP-HPLC method with DAD detector at 225 nm. Deionized water containing 0.5% orthophosphoric acid and methanol was used as the mobile phases A and B. This study showed an optimized analytical method can be effectively utilized to identify and measure anthraquinone compounds.
Reverse Phase High Performance Liquid Chromatography, Anthraquinon, Secondary Metabolites, Method Optimization
In this study, a reverse-phase high performance liquid chromatography (RP-HPLC) method with high separation efficiency, high detection sensitivity and excellent selectivity was successfully developed for the simultaneous quantitative determination of the aloe-emodin, rhein, emodin, chrysophanol, and physcion compounds. These optimum method conditions were determined by studying various columns, mobile phases and compositions, flow rates and column temperatures. The successful separation was carried out with a Supelcosil LC-18 column (250 × 4.6 mm, 5 µm) and a gradient program. The procedure was carried out at 20 °C with the flow rate of 1.0 mL/min and the injection volume of 20 μL utilizing an RP-HPLC method with DAD detector at 225 nm. Deionized water containing 0.5% orthophosphoric acid and methanol was used as the mobile phases A and B. This study showed an optimized analytical method can be effectively utilized to identify and measure anthraquinone compounds.
Reverse Phase High Performance Liquid Chromatography, Anthraquinon, Secondary Metabolites, Method Optimization
Birincil Dil | İngilizce |
---|---|
Konular | Mühendislik |
Bölüm | Makaleler |
Yazarlar |
|
Erken Görünüm Tarihi | 29 Temmuz 2021 |
Yayımlanma Tarihi | 30 Kasım 2021 |
Yayınlandığı Sayı | Yıl 2021, Cilt , Sayı 27 |
Bibtex | @araştırma makalesi { ejosat987628, journal = {Avrupa Bilim ve Teknoloji Dergisi}, eissn = {2148-2683}, address = {}, publisher = {Osman SAĞDIÇ}, year = {2021}, number = {27}, pages = {972 - 984}, doi = {10.31590/ejosat.987628}, title = {Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion}, key = {cite}, author = {Yıldırım Baştemur, Gizem and Akpınar, Reyhan and Perçin Özkorucuklu, Sabriye and Kır, Esengül} } |
APA | Yıldırım Baştemur, G. , Akpınar, R. , Perçin Özkorucuklu, S. & Kır, E. (2021). Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion . Avrupa Bilim ve Teknoloji Dergisi , (27) , 972-984 . DOI: 10.31590/ejosat.987628 |
MLA | Yıldırım Baştemur, G. , Akpınar, R. , Perçin Özkorucuklu, S. , Kır, E. "Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion" . Avrupa Bilim ve Teknoloji Dergisi (2021 ): 972-984 <https://dergipark.org.tr/tr/pub/ejosat/issue/64232/987628> |
Chicago | Yıldırım Baştemur, G. , Akpınar, R. , Perçin Özkorucuklu, S. , Kır, E. "Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion". Avrupa Bilim ve Teknoloji Dergisi (2021 ): 972-984 |
RIS | TY - JOUR T1 - Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion AU - Gizem Yıldırım Baştemur , Reyhan Akpınar , Sabriye Perçin Özkorucuklu , Esengül Kır Y1 - 2021 PY - 2021 N1 - doi: 10.31590/ejosat.987628 DO - 10.31590/ejosat.987628 T2 - Avrupa Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 972 EP - 984 VL - IS - 27 SN - -2148-2683 M3 - doi: 10.31590/ejosat.987628 UR - https://doi.org/10.31590/ejosat.987628 Y2 - 2021 ER - |
EndNote | %0 Avrupa Bilim ve Teknoloji Dergisi Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion %A Gizem Yıldırım Baştemur , Reyhan Akpınar , Sabriye Perçin Özkorucuklu , Esengül Kır %T Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion %D 2021 %J Avrupa Bilim ve Teknoloji Dergisi %P -2148-2683 %V %N 27 %R doi: 10.31590/ejosat.987628 %U 10.31590/ejosat.987628 |
ISNAD | Yıldırım Baştemur, Gizem , Akpınar, Reyhan , Perçin Özkorucuklu, Sabriye , Kır, Esengül . "Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion". Avrupa Bilim ve Teknoloji Dergisi / 27 (Kasım 2021): 972-984 . https://doi.org/10.31590/ejosat.987628 |
AMA | Yıldırım Baştemur G. , Akpınar R. , Perçin Özkorucuklu S. , Kır E. Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion. EJOSAT. 2021; (27): 972-984. |
Vancouver | Yıldırım Baştemur G. , Akpınar R. , Perçin Özkorucuklu S. , Kır E. Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion. Avrupa Bilim ve Teknoloji Dergisi. 2021; (27): 972-984. |
IEEE | G. Yıldırım Baştemur , R. Akpınar , S. Perçin Özkorucuklu ve E. Kır , "Optimization of a Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) Method for Simultaneous Separation of Aloe-Emodin, Rhein, Emodin, Chrysophanol and Physcion", Avrupa Bilim ve Teknoloji Dergisi, sayı. 27, ss. 972-984, Kas. 2021, doi:10.31590/ejosat.987628 |