Blattella germanica (L., 1767) (Blattodea: Ectobiidae)’da thiamethoxam toksisitesine karşı asetilkolinesteraz ve glutatyon S-transferaz enzim aktivitelerinin tepkileri

Öz

Hamam böcekleri birçok hastalık etkeninin vektörü olan önemli zararlılar olup, insektisitlerin bilinçsiz kullanımı bu türlerde geniş çaplı direnç gelişimine neden olmaktadır. Bu çalışma, 2023-2024 yılları arasında at Karamanoğlu Mehmetbey Üniversitesi’nde yürütülmüş olup, thiamethoxam’ın Alman hamamböceği, Blattella germanica (L., 1767) (Blattodea: Ectobiidae) popülasyonları üzerindeki toksik etkilerini belirlemeyi ve asetilkolinesteraz (AChE) ile glutatyon S-transferaz (GST) enzimlerinin tepkilerini araştırmayı amaçlamıştır. Saha popülasyonları (Dokuma, Konyaaltı ve Ahatlı), duyarlı WHO suşuna kıyasla thiamethoxam’a karşı 1.88-3.24 kat arasında değişen düşük düzeyde direnç göstermiştir. WHO grubu, thiamethoxam maruziyeti öncesi ve sonrası AChE aktivitesi bakımından saha gruplarına göre en düşük değeri sergilemiştir (p<0.05). AChE aktivitesinde en belirgin azalma %25 ile WHO grubunda gözlenirken, Ahatlı grubunda bu azalma daha sınırlı olup %13 seviyesinde kalmıştır. Duyarlı WHO suşunda GST aktivitesi saha suşlarına göre daha düşük bulunmuş ve fark anlamlıdır. Thiamethoxam uygulanan Dokuma ve Konyaaltı gruplarında GST aktivitesinde en yüksek değişim (%44) gözlenmiştir. Bununla birlikte, thiamethoxam uygulaması sonrasında en yüksek GST aktivitesi Ahatlı grubunda tespit edilmiştir. Temel bileşen analizi (PCA) ve Hiyerarşik kümeleme analizi (HCA), WHO grubunun biyokimyasal olarak en farklı grup olduğunu doğrulamıştır. Enzimatik biyobelirteçlerin analiz edilmesi, thiamethoxam’ın toksikodinamik özelliklerine dair daha kapsamlı bir anlayışa katkı sağlamaktadır.

Anahtar Kelimeler

• Asetilkolinesteraz
• Alman hamam böceği
• glutatyon-S-transferaz
• direnç
• thiamethoxam

Responses of acetylcholinesterase and glutathione S-transferases activities to thiamethoxam toxicity in Blattella germanica (L., 1767) (Blattodea: Ectobiidae)

Abstract

Cockroaches are significant pests that act as vectors for many disease agents, and the indiscriminate use of insecticides has led to widespread resistance development in these species. This study, conducted at Karamanoğlu Mehmetbey University between 2023 and 2024, aimed to determine the toxic effects of thiamethoxam on German cockroach, Blattella germanica (L., 1767) (Blattodea: Ectobiidae) populations and to investigate the responses of acetylcholinesterase (AChE) and glutathione S-transferase (GST). The field populations (Dokuma, Konyaaltı, and Ahatlı) generally exhibited low levels of resistance to thiamethoxam at a range of 1.88-3.24-fold compared to the susceptible World Health Organization (WHO) strain. The WHO group showed the lowest AChE activity before and after thiamethoxam exposure compared to the field groups (p<0.05). The most significant decrease in AChE activity was observed in the WHO group at 25%, while the decrease in the Ahatlı group was more limited, remaining at 13%. GST activity in the susceptible WHO strain was lower than that of field strains, and the difference was significant. Thiamethoxam-treated Dokuma and Konyaaltı groups exhibited the highest GST activity alterations (44%). However, Ahatlı showed the highest GST activity after the thiamethoxam administration. Principal component analysis (PCA) and Hierarchical clustering analysis (HCA) confirmed the WHO groups were most biochemically differentiated. Analyzing enzymatic biomarkers contributes to more comprehensive insights into the toxicodynamic characteristics of thiamethoxam.

Keywords

• Acetylcholinesterase
• German cockroach
• glutathione-S-transferase
• resistance
• thiamethoxam

Kaynakça

1. Abbasi, E., 2025. Cockroaches as urban pests: Challenges, public health implications, and management strategies. International Journal of Infectious Diseases One Health: 9 (2025): 100086 (1-8).
2. Abdallah, I. S., H. M. Abou-Yousef, E. A. Fouad & E. H. Kandil, 2016. The role of detoxifying enzymes in the resistance of the cowpea aphid (Aphis craccivora Koch) to thiamethoxam. Journal of Plant Protection Research, 56 (1): 76-72.
3. Abdel-Haleem, D. R., A. A. Gad & M. S. Farag, 2020. Larvicidal, biochemical and physiological effects of acetamiprid and thiamethoxam against Culex pipiens L. (Diptera: Culicidae). Egyptian Journal of Aquatic Biology and Fisheries, 24 (3): 271-283.
4. Abdelmoteleb, M. N., A. Z. Mohamed, N. A. Genidy & D. R. Abdel-Haleem, 2023. Computational and toxicological evaluation of thiamethoxam as nicotinic acetylcholine receptor modulator against cowpea aphid, Aphis craccivora Koch. Egyptian Academic Journal of Biological Sciences. A, Entomology, 16 (4): 135-150.
5. Araújo, M. F., E. M. S. Castanheira & S. F. Sousa, 2023. The Buzz on insecticides: A review of uses, molecular structures, targets, adverse effects, and alternatives. Molecules, 28 (8): 3641 (1-16).
6. Balkan, T. & K. Kara, 2020. Neonicotinoid resistance in adults and nymphs of Bemisia tabaci (Genn, 1889) (Hemiptera: Aleyrodidae) populations in tomato fields from Tokat, Turkey. Turkish Journal of Entomology, 44 (3): 319-331.
7. Benchaâbane, S., A. S. Ayad, W. Loucif-Ayad & N. Soltani, 2022. Multibiomarker responses after exposure to a sublethal concentration of thiamethoxam in the African honeybee (Apis mellifera intermissa). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 257 (2022): 109334 (1-4).
8. Bk, S. K., T. Moural & F. Zhu, 2022. Functional and structural diversity of insect glutathione S-transferases in xenobiotic adaptation. International Journal of Biological Sciences, 18 (15): 5713-5723.

9. Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72 (1-2): 248-254.
10. Chai, R. Y. & C. Y. Lee, 2010. Insecticide resistance profiles and synergism in field populations of the German cockroach (Dictyoptera: Blattellidae) from Singapore. Journal of Economic Entomology, 103 (2): 460-471.
11. de Souza, F. C., L. Miotelo, G. Maloni, I. V. R. Otero, R. C. F. Nocelli & O. Malaspina, 2024. Thiamethoxam toxicity on the stingless bee Friesiomelitta varia: LC50, survival time, and enzymatic biomarkers assessment. Chemosphere, 363 (2024): 142853 (1-7).
12. Decio, P., L. Miotelo, F. D. Campos Pereira, T. C. Roat, M. A. Marin-Morales & O. Malaspina, 2021. Enzymatic responses in the head and midgut of Africanized Apis mellifera contaminated with a sublethal concentration of thiamethoxam. Ecotoxicology and Environmental Safety, 223 (2021): 112581 (1-8).
13. Dentzman, K., D. Franklin, E. Avemegah & J. R. Goldberger, 2025. An overview of agricultural neonicotinoid regulation in the EU, Canada, and the United States. Pest Management Science, 81(12): 7593-7601.
14. Du, Y., S. Scheibener, Y. Zhu, M. Portilla & G. V. Reddy, 2024. Biochemical and molecular characterization of neonicotinoids resistance in the tarnished plant bug, Lygus lineolaris. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 275 (2024): 109765 (1-8).
15. Ellman, G. L., D. K. Courtney, V. Andres Jr. & R. M. Featherstone, 1961. A new rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7 (2): 88-95.
16. Erdogan, A. & O. Kocak, 1989. Hamamböceği, Blattella germanica (L), popülasyonlarında nimf süresi ve ergin ömür uzunluğu ile ilgili araştırmalar. Hacettepe Üniversitesi Eğitim Fakültesi Dergisi, 4 (4): 235-238. (in Turkish with abstract in English)
17. Fardisi, M., A. D. Gondhalekar & M. E. Scharf, 2017. Development of diagnostic insecticide concentrations and assessment of insecticide susceptibility in German cockroach (Dictyoptera: Blattellidae) field strains collected from public housing. Journal of Economic Entomology, 110 (3): 1210-1217.
18. Fardisi, M., A. D. Gondhalekar, A. R. Ashbrook & M. E. Scharf, 2019. Rapid evolutionary responses to insecticide resistance management interventions by the German cockroach (Blattella germanica L.). Scientific Reports, 9 (1): 82-92.
19. Garrett, D. A., J. S. Agee, E. R. Gremminger & W. E. Morgan, 1968. Resistance levels of Izmir, Turkey bedbugs and cockroaches to insecticides 1966-67. Ft. Belvoir Defense Technical Information Center, Professional Report, No: 68 (7): 24 pp.
20. Guzman, J. & A. Vilcinskas, 2020. Bacteria associated with cockroaches: health risk or biotechnological opportunity?. Applied Microbiology and Biotechnology, 104 (24): 10369-10387.
21. Habig, W. H. & W. B. Jakoby, 1981. Assays for differentiation of glutathione-S-transferase. Methods in Enzymology, 77: 398-405.
22. Kaya, C., N. S. Çağatay, J. T. Margaritopoulos, J. Vontas, R. Atlihan & N. Güz, 2023. Neonicotinoid resistance in populations of the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae) in cotton plantation areas of Turkey. Turkish Journal of Agriculture and Forestry, 47 (5): 623-632.
23. Keswani, C., H. Dilnashin, H. Birla, P. Roy, R. K. Tyagi, D. Singh, D. R. Vishnu, T. Minkina & S. P. Singh, 2022. Global footprints of organochlorine pesticides: a pan-global survey. Environmental Geochemistry and Health, 44 (1): 149-177.
24. Li, Z., Y. Qin, R. Jin, Y. Zhang, Z. Ren, T. Cai, C. Yu, Y. Liu, Y. Cai, Q. Zeng, H. Wan & J. Li, 2021. Insecticide resistance monitoring in field populations of the whitebacked planthopper Sogatella furcifera (Horváth) in China, 2019-2020. Insects, 12 (12): 1078 (1-15).
25. Lu, W., Z. Liu, X. Fan, X. Zhang, X. Qiao & J. Huang, 2022. Nicotinic acetylcholine receptor modulator insecticides act on diverse receptor subtypes with distinct subunit compositions. PLoS Genetics, 18 (1): e1009920 (1-15).
26. Maienfisch, P., M. Angst, F. Brandl, W. Fischer, D. Hofer, H. Kayser, W. Kobel, A. Rindlisbacher, R. Senn, A. Steinemann & H. Widmer, 2001. Chemistry and biology of thiamethoxam: A second-generation neonicotinoid. Pest Management Science, 57 (10): 906-913.
27. Maloni, G., L. Miotelo, I. V. R. Otero, F. C. de Souza, R. C. F. Nocelli & O. Malaspina, 2025. Acute toxicity and sublethal effects of thiamethoxam on the stingless bee Scaptotrigona postica: Survival, neural morphology, and enzymatic responses. Environmental Pollution, 369 (2025): 125864 (1-10).
28. Öz, E., H. Çetin & A. Yanıkoğlu, 2021. Investigation of resistance to synthetic pyrethroids in Blattella germanica L, 1767 (Blattodea: Ectobiidae) and Periplaneta americana L, 1758 (Blattodea: Blattidae) populations in Turkey. Turkish Journal of Entomology, 45 (3): 361-370.
29. Pavlidi, N., J. Vontas & T. Van Leeuwen, 2018. The role of glutathione S-transferases (GSTs) in insecticide resistance in crop pests and disease vectors. Current Opinion in Insect Science, 27: 97-102.
30. Polat, B. & H. Çetin, 2020. Toxicity of thiamethoxam and piperonyl butoxide combination against some strains of house fly Musca domestica L. (Diptera) in Turkey. Acta Zoologica Bulgarica, 72 (2): 321-324.
31. Shi, D., T. Wang, H. Lv, X. Li, H. Wan, S. He, H. You, J. Li & K. Ma, 2023. Insecticide resistance monitoring and diagnostics of resistance mechanisms in cotton‐melon aphid, Aphis gossypii Glover in Central China. Journal of Applied Entomology, 147 (6): 392-405.
32. Sims, S. R. & A. G. Appel, 2007. Linear alcohol ethoxylates: Insecticidal and synergistic effects on German cockroaches (Blattodea: Blattellidae) and other insects. Journal of Economic Entomology, 100 (3): 871-879.
33. Tang, Q., T. Bourguignon, L. Willenmse, E. De Coninck & T. Evans, 2019. Global spread of the German cockroach, Blattella germanica. Biological Invasions, 21 (3): 693-707.
34. Tisgratog, R., C. Panyafeang, S. H. Lee, M. K. Rust & C. Y. Lee, 2023. Insecticide resistance and its potential mechanisms in field-collected German cockroaches (Blattodea: Ectobiidae) from Thailand. Journal of Economic Entomology, 116 (4): 1321-1328.
35. Uçkun, M., E. Yoloğlu, A. Alkan Uçkun & Ö. Barım, 2021. Acute toxicity of insecticide thiamethoxam to crayfish (Astacus leptodactylus): Alterations in oxidative stress markers, ATPases and cholinesterase. Acta Chimica Slovenica, 68 (3): 1-11.
36. Yan, S. H., J. H. Wang, L. S. Zhu, A. M. Chen & J. Wang, 2016. Thiamethoxam induces oxidative stress and antioxidant response in zebrafish (Danio rerio) livers. Environmental Toxicology, 31 (12): 2006-2015.
37. Yang, S. H., J. H. Wang, L. S. Zhu, A. M. Chen & J. Wang, 2016. Glutathione S-transferases are involved in thiamethoxam resistance in the field whitefly Bemisia tabaci Q (Hemiptera: Aleyrodidae). Pesticide Biochemistry and Physiology, 134 (2016): 73-78.
38. Yasoob, H., N. Abbas, Y. Li & Y. Zhang, 2018. Selection for resistance, life history traits and the biochemical mechanism of resistance to thiamethoxam in the maize armyworm, Mythimna separate (Lepidoptera: Noctuidae). Phytoparasitica, 46 (5): 627-634.