Toxicological and behavioral effects of some plant extract on Colorado potato beetle, Leptinotarsa decemlineata Say, 1824 (Coleoptera: Chrysomelidae)

Abstract

Repellent, ovicidal and oviposition deterrent effects of six plant extracts (Heracleum platytaenium Boiss (Apiaceae), Humulus lupulus L. (Cannabaceae), Achillea millefolium L. (Asteraceae), Acanthus dioscoridis L. (Acanthaceae), Phlomoides tuberosa (L.) Moench (Lamiaceae), Bifora radians Bieb. (Apiaceae)) were tested on Leptinotarsa decemlineata Say, 1824 (Coleoptera: Chrysomelidae) under laboratory conditions. Methanol extracts prepared from plant vegetative components were tested on L. decemlineata. Heracleum platytaenium extract was significantly more toxic against the egg stage than all other extracts, except for A. millefolium 5 d after treatment. It was followed by A. millefolium extract reducing the egg hatch rate to 15%. Significant mortality was not observed in the case of other plant extracts. In the second series of experiments, different dose-response bioassays with H. platytaenium against L. decemlineata eggs were conducted. The lowest egg hatch rate of 1% was observed at 7.5% [w/v (plant extract/acetone]. The greatest oviposition deterrent effect was seen with the H. platytaenium extract treatment, which resulted in no egg laying. Plant extracts showed a high level of repellent activity to L. decemlineata and their activity increased with extended incubation time. The greatest repellency was observed with the A. millefolium extract treatment, which gave 0.01% repellency in the first 15 min. These results show that H. platytaenium extract could be a useful toll in the control of L. decemlineata.

Keywords

• Biopesticide
• Colorado potato beetle
• ovicidal effect
• plant extract
• repellency

Bazı bitki ekstraktlarının Patates böceği [Leptinotarsa decemlineata Say,1824 (Coleoptera: Chrysomelidae)]’ne karşı toksikolojik ve davranışsal etkileri

Öz

Altı bitki ekstraktının [Heracleum platytaenium Boiss (Apiaceae), Humulus lupulus L. (Cannabaceae), Achillea millefolium L. (Asteraceae), Acanthus dioscoridis L. (Acanthaceae), Phlomoides tuberosa (L.) Moench (Lamiaceae), Bifora radians Bieb. (Apiaceae)], Leptinotarsa decemlineata Say 1824 (Coleoptera: Chrysomelidae)’ye karşı repellent, ovisidal ve yumurta bırakmayı engelleyici etkileri test edilmiştir. Bitkilerin vejetatif aksamlarından hazırlanan methanol ekstraktları L. decemlineata üzerinde test edilmiştir. En yüksek ovisidal etki beşinci gün itibari ile H. platytaenium uygulamasında gözlemlenmiş ve bunu A. millefolium ekstraktının etkisi izlemiştir. Heracleum platytaenium ekstraktının etkinliğini %15 yumurta açılım oranı ile A. millefolium ekstraktının etkinliği takip etmiştir. Diğer bitki ekstraktlarında önemli ölüm gözlenmemiştir. İkinci seri denemelerde Patates böceğinin yumurtalarına karşı H. platytaenium’un doz-etki çalışmaları yürütülmüştür. Önemli derecede düşük yumurta açılım oranı sadece %7.5 [w/v (bitki ekstraktı/aseton)] konsantrasyonda %1 etki ile gözlemlenmiştir. En yüksek yumurta bırakmayı engelleyici etki H. platytaenium ekstraktında görülmüş ve bu muamelede hiç yumurta bırakılmamıştır. Bitki ekstraktları yüksek oranda repellent aktivite göstermiş ve bu aktivite inkübasyon süresine bağlı olarak artmıştır. Test edilen bitki ekstraktları arasında en yüksek derecede uzaklaştırıcı etki %0.01’lik oransal değer ile ilk 15 dakikada A. millefolium ekstraktında saptanmıştır. Bu sonuçlar H. platytaenium ekstraktının L. decemlineata kontrolünde önemli bir potansiyele sahip olduğunu ortaya koymuştur.

Anahtar Kelimeler

• Biyopestisit
• Patates böceği
• yumurta bırakmayı engelleyici etki
• bitki ekstraktı
• repellent

Kaynakça

1. Alkan, M. & A. Gökçe, 2012. Tanacetum abrotanifolium (L.) DRUCE (Asteraceae)’ un gövde ve çiçek ekstraktlarının Sitophilus granarius ve Sitophilus oryzae (Col., Curculionidae)’ye olan kontakt ve davranışsal etkileri. Turkish Journal of Entomology, 36 (3): 377-389.
2. Alyokhin, A. 2009. Colorado potato beetle management on potatoes: Current challanges and future prospects fruit, vegetable and cereal. Science and Biotechnology, 3 (Special Issue 1): 10-19.
3. Arivoli, S. & S. Tennyson, 2013. Ovicidal activity of plant extracts against Spodoptera litura (Fab) (Lepidoptera: Noctiduae). Bulletin of Environment, Pharmacology and Life Sciences, 2(10):140-145.
4. Booth, R.G., M.L. Cox & R.B. Madge, 1990. Lie guides to insects of importance to man, 3. Coleoptera. Printed in the UK at the University Pres, Cambridge.
5. Borror, D.J. & D.M. Delong, 1966. An introduction to the study of insects. Holt Rinehart and Winston Inc. Newyork. 819.
6. Carroll, M.J. & M.R. Berenbaum, 2002. Behavioral responses of the parsnip webworm to hostplant volatiles. Journal of Chemical Ecology, 28: 2191-2201.
7. Chiasson, H., V. Groison, C. Vincent & A. Bélanger, 1994. Determination of the insecticidal properties of Tanacetum vulgare on the Colorado beetle (Leptinotarsa decemlineata). Resume des Recherches, Centre de Rechercheet de Developpementen Horticulture, 23:43-44.
8. Christie, R.D., A.C. Sumalde, J.T. Schutz & N.C. Gudmestad, 1991. Insect transmission of the bacterial ring rot pathogen. American Potato Journal, 68:363-372.

9. Dehghani, A. & K. Ahmadi, 2013. Repellence and anti-oviposition activities of plant products on greenhouse whitefly. Pharmacognosy Communications, 3(2):02-05.
10. Gökçe, A, L.L. Stelinski & M.E. Whalon, 2005, Behavioral and electrophysiological responses of leafroller moths to selected plant extracts. Environmental Entomology, 34 (6):1426-1432.
11. Gökce, A., M.E. Whalon, H. Cam, Y. Yanar, I. Demirtas & N. Goren, 2006. Plant extract contact toxicities to various developmental stages of Colorado potato beetles. Annals of Applied Biology, 149:197–202.
12. Gökçe, A., R. Isaacs &M.E. Whalon, 2011. Ovicidal, larvicidal and anti-ovipositional activities of Bifora radians and other plant extracts on the grape berry moth Partalobesia viteana (Clemens). Journal of Pest Science, 84 (4):487-493.
13. Hare, J.D.,1990. Ecology and management of the Colorado potato beetle. Annual Review of Entomology, 35:81–100.
14. Hassan, E. & A. Gökçe, 2014. Production and Consumption of Biopesticides in Advances in Plant Biopesticides. Ed. Singh D. Springer, 361-379pp
15. Hough-Goldstein, J.A., 1990. Antifeedant effects of common herbs on the Colorado potato beetle (Coleoptera:Chrysomelidae). Enviromental Entomology, 19:234-238.
16. Hsiao, T.H., 1978. Host-plant adaptations among geographic populations of the Colorado potato beetle. Entomologia Experimentalis et Applicata, 24:237-247.
17. Ioannidis, P.M., E. Grafius & M.E. Whalon, 1991. Patterns of insecticide resistance to Azinphosmethyl, Carbofuran, and Permethrin in the Colorado potato beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 84:1417-1423.
18. Iscan, G., T. Ozek, G. Ozek, A. Duran & K.H.C. Baser, 2004. Essential oils of three species of Heracleum. Anticandidal Actaivity. Chem. Nat. Comp., 40 (6):544-547.
19. Jeyasankar, A., K. Elumalai, N. Raja & S. Ignacimuthu, 2013. Effect of plant chemicals on oviposition deterrent and ovicidal activities against female moth, Spodoptera litura (Fab.) (Lepidoptera: Noctuidae). International Journal of Agricultural Science Research, 2 (6):206-213.
20. Jolivet, P., E. Petipierre & T.H. Hasiao, 1988. Biology of Chrysomelidae. Series Entomologicia, 42, Kluver Academic Puplishers.
21. Kısmalı, Ş., 1973. İzmir ili ve çevresinde kültür bitkilerinde zarar yapan Chrysomelinae ve Halticinae (Coleoptera, Chrysomelidae) alt familyalarina ait türler, tanınmaları, konukçuları, yayılışları ve kısa biyolojileri üzerinde araştırmalar. E.Ü. Zir. Fak. Derg, 10 (2): 341-378.
22. Kürkçüoğlu, M., T. Özek, K.H.C. Başer & H. Malyer, 1995. Composition of the essential oil of Heracleum platytenium Boiss. from Turkey. J.Essent. Oil Res, 7:69-70
23. Mateeva, A., 1998. Some essential oils as effective plant protection means against Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Proceedings: 50th international symposium on crop protection, Gent, Belgium, 5 May 1998, Part II
24. Mota-Sanchez, D., M.E. Whalon, E. Grafius & R. Hollingworth, 2000. Resistance of Colorado potato beetle to Imidacloprid. Resistance Pest Management Newsletter, 11:31-34.
25. Pavela, R., 2004. Repellent effect of ethanol extracts from plants of the family Lamiaceae on Colorado Potato Beetle adults (Leptinotarsa decemlineata Say). National Academy Science Letters, 27(5-6):195-203.
26. Przybylski, Z., 2002. The results of the research on the repellent effect of Tanacetum vulgare on Colorado potato beetle (Leptinotarsa decemlineata Say). Journal of Plant Protection Research, 42 (4):309-314.
27. Rajkumar, S. & A. Jabanesan, 2005. Oviposition deterrent and skin repellent activities of Solanum trilobatum leaf extract against the malarial vector Anopheles stephensi. J Insect Sci., 5:15.
28. Sarbu, C., I. Oltean, G. Morar, C. Socaciu, M. Porca, O. Bobis & O Gheoltan, 2004. The influence of some natural extracts from plants on the Colorado bug larvae behavior (Leptinotarsa decemlineata Say.) in the management of ecological production of potatoes. Seria Agricultura, 60:106-109.
29. Schearer, W.R., 1984. Componenets of oil of Tansy (Tanacetum vulgare) that repel Colorado Potato Beetles (Leptinotarsa decemlineata). Journal of Natural Products, 47(6):964-969.
30. Scott, I.M., H. Jensen, J.G. Scott, M.B. Isman, J.T. Arnason & B.J.R. Philogene, 2003. Botanical insecticides for controlling agricultural pests: Piperamides and the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera : Chrysomelidae). Archives of Insect Biochemistry and Physiology, 54: 212-225.
31. Scott, I.M., H. Jensen, L. Nicol, R. Bradbuty, P. Sanches-Vindas, L. Poveda, J.T. Arnason & B.J.R. Philogene, 2004. Efficiacy of Piper (Piperaceae) extracts for control of common home and garden insect pests. Journal of Economic Entomology, 97:1390-1403.
32. Sezer, B., P. Ozalp, 2011. Azadirachtinin Galleria mellonella larvalarinda total glikojen miktarina etkisi. Ekoloji 20: 67-72.
33. Singh, H., S. Dixit, P.C. Verma & P.K. Singh, 2014. Evaluation of total phenolic compounds and insecticidal and antioxidant activities of tomato hairy root extract. J. Agric. Food Chem, 62 (12):2588–2594.
34. Stewart, J.G., G.G. Kennedy & A.V. Sturz, 1997. Incidence of insecticides resistance in population of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera : Chrysomelidae) on Prince Edward Island. Canadian Entomologist, 129: 21-26.
35. Tomasek, J. & P. Dvorak, 2009. Alternative protection of biopotatoes in organic farming. Seria Agronomia, 52 (1):133-135.
36. Ur-Rehman, J., G. Jilani, M.A. Khan, R. Masih & S. Kanvil, 2009. Repellent and oviposition deterrent effects of indigenous plant extracts to Peach fruit fly, Bactrocerazonata saunders (Diptera: Tephritidae). Pakistan J. Zool., 41(2):101-108.
37. Wawrzyniak, M. & R. Lamparski, 2008. Evaluation of the effect of extracts from selected plants from the Labiatae family on the feeding and development of Colorado potato beetle (Leptinotarsa decemlineata Say.). Progress in Plant Protection, 48 (4):1374-1377.
38. Whalon, M.E., D. Mota-Sanchez, R. Hollingworth & L. Duynslager, 2013. Arthropod Pesticide Resistance Database. (Web page: http://www.pesticideresistance.com/), (Accessed date: 11.05.2015).
39. Wyrostkiewicz, K., 1987. Effect of aqueous plant extracts on feeding by the Colorado potato beetle (L. decemlineata Say.). Materiay Sesji Instytu Ochrony Roślin, 27 (2):39-42.