Samsun’da Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae)'dan yeni bir bakülovirüs izolatının ve farklı konukçularda patojenitesinin belirlenmesi

Öz

Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae), Avrupa, Asya ve Kuzey Afrika'ya dağılmış dünya çapında bir zararlıdır. Elma, armut, erik, söğüt ve meşede ekonomik kayıplara neden olur. Bu çalışmada, Türkiye'nin Orta Karadeniz Bölgesi’nde bulunan Samsun'dan 2015-2016 yıllarında toplanan M. neustria larvalarından bakulovirüs izole edilmiştir. Ölü larvalardan elde edilen oklüzyon badilerin (OB) elektron mikroskobu analizi, çoklu nükleokapsidlere sahip yeni bir izolat (ManeNPV-T4) olduğunu gösterdi. Kimura iki-parametre analizi ve filogenetik ağaç, ManeNPV-T4 izolatından elde edilen kısmi lef-8, lef-9 ve polh genlerinin birleştirilmiş nükleotit ve amino asit dizilerine dayanarak, diğer 51 baculovirüsünki ile karşılaştırıldı. Üçüncü evre M. neustria larvalarına uygulanan insektisidal aktivite testleri sonucunda %48 ile %100 arasında ölüm gözlendi. ManeNPV-T4'ün M. neustria için LC₅₀ değeri 0,78 x 10³ OB/ml olarak belirlendi. Ek olarak, viral izolat Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae), Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) ve Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) larvaları üzerinde %50’den düşük ölüm oranı oluşturdu. Sonuç olarak, bu yeni nükleopolihedrovirüs M. neustria ve diğer lepidopter larvalarına karşı da enfektiviteye sahiptir.

Anahtar Kelimeler

• Bakülovirüs,tespit,Malacosoma neustria,ölüm oranı

Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts

Abstract

Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) causes economic losses in apple, pear, plum, willow, oak and other economically important trees. In this study, an alphabaculovirus was isolated from the larval population of M. neustria from Samsun in the central Black Sea Region of Turkey between 2015 and 2016. Electron microscope analysis of occlusion bodies (OBs) obtained from dead larvae showed that the nucleocapsids of a new isolate (ManeNPV-T4) are multiply enveloped. The Kimura two-parameter analysis and the phylogenetic tree were performed based on concatenated nucleotide and amino acid sequences of the partial lef-8, lef-9 and polh genes from ManeNPV-T4 isolate compared to those of other 51 baculoviruses. Insecticidal activity tests against third instar M. neustria larvae produced 48 to 100% mortalities. The LC₅₀ of ManeNPV-T4 was 0.78 x 10³ OBs/ml in M. neustria. Additionally, the isolate caused mortalities lower than 50% in Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae), Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) and Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) larvae. Consequently, the new nucleopolyhedrovirus is infectious on M. neustria larvae and other lepidopterans.

Keywords

• Baculovirus,Malacosoma neustria,identification,mortality

Kaynakça

1. Bergold, G. H., 1953. Insect viruses. Advances in Virus Research, 1: 91-139.
2. Brodeur, J., 2012. Host specificity in biological control: insights from opportunistic pathogens. Evolutionary Applications, 5: 470-480.
3. De Moraes, R. R. & J. E. Maruniak, 1997. Detection and identification of multiple baculoviruses using the polymerase chain reaction (PCR) and restriction endonuclease analysis. Journal of Virological Methods, 63: 209-217.
4. Demir, I., R. Nalçacioǧlu, L. Mohammad Gholizad & Z. Demirbaǧ, 2013. Characterization of a new isolate of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey. Turkish Journal of Biology, 37: 385-391.
5. Demir, I., R. Nalçacioǧlu, L. Mohammad Gholizad & Z. Demirbaǧ, 2014. A highly effective nucleopolyhedrovirus against Malacosoma spp. (Lepidoptera: Lasiocampidae) from Turkey: isolation, characterization, phylogeny, and virulence. Turkish Journal of Agriculture and Forestry, 38: 462-470.
6. Gencer, D., R. Nalcacioglu, Z. Demirbag & I. Demir, 2018. Complete genome sequence analysis of the Malacosoma neustria nucleopolyhedrovirus from Turkey. Virus Genes, 54: 706-718.
7. Granados, R. R., 1980. Infectivity and mode of action of Baculoviruses. Biotechnology and Bioengineering, 22: 1377-1405.
8. Haase, S., A. Sciocco-Cap & V. Romanowski, 2015. Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives. Viruses, 7: 2230-2267.

9. Herniou, E. A., J. A. Olszewski, J. S. Cory & D. R. O’Reilly, 2003. The genome sequence and evolution of baculoviruses. Annual Review of Entomology, 48: 211-234.
10. Herniou, E. A., J. A. Olszewski, D. R. O’Reilly & J. S. Cory, 2004. Ancient coevolution of Baculoviruses and their insect hosts. Journal of Virology, 78: 3244-3251.
11. Jankevica, L., Z. Cudare & V. Ose, 1998. New isolate of Malacosoma neustria nuclear polyhedrosis virus in Latvia. Journal of Invertebrate Pathology, 71: 283-285.
12. Jankevica, L., M. Kropa, N. Savenkovs & R. Jankevics, 2002. Presence of nucleopolyhedroviruses in natural populations of Malacosoma neustria L. (Lepidoptera, Lasiocampidae). Latvijas Entomologs, 39: 30-37.
13. Jehle, J. A., M. Lange, H. Wang, Z. Hu, Y. Wang & R. Hauschild, 2006. Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology, 346: 180-193.
14. Lipa, J. J., S. M. Gershenson & A. P. Gudz-Gorban, 1968. Electron microscopy, histopathology and pathogenicity of nuclear polyhedrosis virus of Malacosoma neustria L. Acta Microbiologica Polonica, 17: 191-201.
15. Muñoz, D., J. M. Vlak & P. Caballero, 1997. In vivo recombination between two strains of the genus Nucleopolyhedrovirus in its natural host, Spodoptera exigua. Applied and Environmental Microbiology, 63: 3025-3031.
16. Murillo, R., M. D. Muñoz, J. J. Lipa & P. Caballero, 2001. Biochemical characterization of three nucleopolyhedrovirus isolates of Spodoptera exigua and Mamestra brassicae. Journal of Applied Entomology, 125: 267-270.
17. Ozbek, H. & O. Calmasur, 2005. A review of insects and mites associated with roses in Turkey. Acta Horticulturae, 690: 167-174.
18. Ozbek, H. & S. Coruh, 2010. Egg parasitoids of Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Erzurum province of Turkey. Turkish Journal of Entomology, 34: 551-560.
19. Poitout, S. & R. Bues, 1974. Elevage de chenilles de vingt huit especes de lepidoptere Noctuidae et de deux especes d’ Arctiidae sur milieu artificiel simple. Particularites de l’ elevage selon les especes. Annales de Zoologie Ecologie Animale, 6: 431-441.
20. Ponsen, M. B., S. Henstra & C. Scheer, 1964. Electron microscope observations of nuclear polyhedra from Malacosoma neustria (Lepidoptera: Lasiocampidae). Netherlands Journal of Plant Pathology, 70: 101-104.
21. Progar, R. A., M. J. Rinella, D. Fekedulegn & L. Butler, 2010. Nuclear polyhedrosis virus as a biological control agent for Malacosoma americanum (Lepidoptera: Lasiocampidae). Journal of Applied Entomology, 134: 641-646.
22. Püntener, W., 1981. Manual for Field Trials in Plant Protection (2nd Edition). Agricultural Division, Ciba-Geigy Limited, Basel, Switzerland, 205 pp.
23. Reed, C., I. S. Otvos, R. Reardon, I. Ragenovich & H. L. Williams, 2003. Effects of long-term storage on the stability of OpMNPV DNA contained in TM Biocontrol-1. Journal of Invertebrate Pathology, 84: 104-113.
24. Rohrmann, G., 2013. Baculovirus Molecular Biology (3rd Edition). National Center for Biotechnology Information, Bethesda, Maryland, USA, 154 pp.
25. Wennmann, T. J., J. Keilwagen & J. A. Jehle, 2018. Baculovirus Kimura two-parameter species demarcation criterion is confirmed by the distances of 38 core gene nucleotide sequences. Journal of General Virology, 99: 1307-1320.
26. Yaman, M., 2003. Isolation and Characterization of Virus from Malacosoma neustria (Lepidoptera: Lasiocampidae) and Its Use in Microbial Control. Graduate School of Natural and Applied Science, Karadeniz Technical University, (Unpublished) PhD Thesis, Trabzon, Turkey, 83 pp.