Molecular identification of root-knot nematode Meloidogyne incognita from kiwi fruit orchards in Ordu province, Turkey

Abstract

Root-knot nematodes (Meloidogyne spp.) are one of the major groups of nematodes identified on kiwi fruit (Actinidia deliciosa Chev.). This investigation was conducted to identify root-knot nematodes encountered the in kiwi fruit orchards in the Ordu province of Turkey by means of Polymerase Chain Reaction (PCR). For this purpose, infected plant root samples were collected from 17 kiwi fruit orchards located in Center, Perşembe, Ünye, Gülyalı, İkizce and Ulubey districtsof the province during the years 2011-2012.Females directly obtained from the infected roots of orchard populations were used for the molecular identifications. It was carried out with an identification key using ribosomal DNA (rDNA) region, Intergenic Spacer (IGS-2) region between 5S-18S genes, and the speciesspecific Sequence Characterized Amplified Region (SCAR) markers. All samples from the districts of Ordu province were identified as Meloidogyne incognita by the exact size of 502 bp amplicons by SEC1F/SEC1R scar primers. This study was the first detailed investigation both in the province and country on kiwi fruit

Keywords

• Actinidia deliciosa, Kiwi, Meloidogyne incognita, rDNA , Root-knot nematodes, SCAR

Ordu ili kivi bahçelerinde görülen kök-ur nematodu Meloidogyne incognita'nın moleküler teşhisi

Öz

Kök-ur nematodları, kivi bitkisi (Actinidia deliciosa)’nde zararlı olan önemli nematod gruplarından birisidir. Bu çalışma, Ordu ili kivi bahçelerinde görülen kök-ur nematodlarının, PCR (Polymerase Chain Reaction) yöntemi ile teşhisini gerçekleştirmek için yapılmıştır. Bu amaçla 2011-2012 yıllarında Ordu ili Merkez, Perşembe, Ünye, Gülyalı, İkizce, Ulubey ilçelerini kapsayacak şekilde 17 farklı kivi bahçesinden bulaşık kök örnekleri toplanmıştır. Kök-ur nematodu ile enfekteli kivi köklerinden elde edilen dişiler doğrudan teşhis için kullanılmıştır. Moleküler teşhisler, teşhis anahtarı kapsamında sunulan 5S-18S genleri arasındaki rDNA bölgesi IGS-2 için kullanılan primerler ve türe özgü SCAR primerlerinin kullanılması suretiyle gerçekleştirilmiştir. Ordu ili kivi bahçelerinden elde edilen bütün örneklerin teşhisi sonucunda tespit edilen tür Meloidogyne incognita olmuş ve SEC1F/SEC1R türe özgü primerleri 502 bp değerinde amplicon vermiştir. Bu çalışma, kivi bitkisi üzerinde ilk detaylı çalışma niteliğindedir

Anahtar Kelimeler

• Actinidia deliciosa, Kivi, Meloidogyne incognita, rDNA, Root-knot nematodes, SCAR

Kaynakça

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