Growth inhibitory effects of bio- and synthetic insecticides on Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae)

Abstract

The study aimed to found out the potential efficacy of some bio- and synthetic insecticides used against Tuta absoluta (Lepidoptera: Gelechiidae) in tomato greenhouses. Chlorantraniliprole+abamectin, metaflumizone, azadirachtin, emamectin benzoate, spinosad, Bacillus thuringiensis and B. thuringiensis+azadirachtin mixture were applied by spraying method on tomato leaf, which 5 third-stage larvae existed on. Distilled water was used as a control to compare with insecticides. Each of the applications was performed in six replications. The assessments were done at the 1, 3, 5 and 7 DAA (Days After Application) according to the number of live individuals and larval weight. Mean numbers of live metaflumizone-, azadirachtin-, Bacillus thuringiensis- and B. thuringiensis+azadirachtintreated larvae at the 7 DAA were recorded as 0.33, 1.17, 0.67 and 0.33, respectively. Growth inhibition indexes of same larvae groups were 0.64, 0.17, 0.65 and 0.80, respectively. All individuals in chlorantraniliprole+abamectin-, emamectin benzoate- and spinosad-treated larvae were died at the 7 DAA. Growth inhibition indexes of these larvae groups were 0.97, 0.82 and 0.89, respectively. Pupations of metaflumizone-, azadirachtin-, Bacillus thuringiensis- and B. thuringiensis+azadirachtin-treated larvae were determined as 6.67, 20.0, 16.67 and 6.67 percent, respectively. Azadirachtin-treated larvae showed the success of adult emergence in the rate of 6.67%. However, none of the larvae treated with other insecticides could show the success of adult emergence. Mean weight of the control larvae group gradually decreased and also, the rate of pupation and adult emergence in this group were 56.67 and 50.00 percent. In addition, it was determined that B. thuringiensis+azadirachtin mixture constituted a synergistic effect on the pest

Keywords

• Azadirachtin, Bacillus thuringiensis, emamectin benzoate, spinosad, Tuta absoluta

Biyo- ve sentetik insektisitlerin Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae)’ya gelişme engelleyici etkileri

Öz

Bu çalışmada Tuta absoluta (Lepidoptera: Gelechiidae)’ya karşı seralarda yaygın olarak kullanılan sentetik insektisitlerin ve biyo-insektisitlerin potansiyel etkinliği ortaya çıkarılmaya çalışılmıştır. Denemede Chlorantraniliprole+abamectin, metaflumizone, azadirachtin, emamectin benzoate, spinosad, Bacillus thuringiensis ve B. thuringiensis+azadirachtin karışımı üzerinde 5 adet üçüncü dönem larva bulunan domates yapraklarına püskürtme yöntemiyle uygulanmıştır. Kontrol uygulaması olarak da saf su kullanılmıştır. Her bir uygulama altı tekerrürlü olarak gerçekleştirilmiştir. Değerlendirmeler uygulamalardan sonraki 1., 3., 5. ve 7. günlerde canlı birey sayıları ve larva ağırlıkları üzerinden yapılmıştır. Metaflumizone, azadirachtin, Bacillus thuringiensis ve B. thuringiensis+azadirachtin ile muamele edilen larvaların canlılık oranları 7. gün sonunda sırasıyla 0,33, 1,17, 0,67 ve 0,33 olarak kaydedilmiştir. Aynı larvaların gelişme gerileme indeksi sırasıyla 0,64, 0,17, 0,65 ve 0,80’dir. Chlorantraniliprole+abamectin, emamectin benzoate ve spinosad ile muamele edilen larvaların tamamı 7. gün sonunda ölmüştür. Bu larvaların gelişme gerileme indeksi ise sırasıyla 0,97, 0,82 ve 0,89’dur. Metaflumizone, azadirachtin, Bacillus thuringiensis ve B. thuringiensis+azadirachtin ile muamele edilen larvaların pupa olma oranları sırasıyla %6,67, 20,0, 16,67 ve 6,67 olarak belirlenmiştir. Azadirachtin ile muamele edilen larvalar %6,67 oranında ergin olma başarısı gösterirken diğer insektisitler ile muamele edilen larvaların hiçbirisi ergin olma başarısı gösterememiştir. Kontrol grubundaki larvalarda ortalama ağırlık giderek azalmıştır. Ayrıca bu grupta ki larvaların pupa ve ergin olma oranları %56,67 ve %50,00 olarak bulunmuştur. Bunun yanı sıra B. thuringiensis+azadirachtin karışımının sinerjistik bir etki meydana getirdiği tespit edilmiştir.

Anahtar Kelimeler

• Azadirachtin, Bacillus thuringiensis, emamectin benzoate, spinosad, Tuta absoluta

Kaynakça

1. Abbott, W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
2. Abdelgaleil, S.A.M. & A.F. El-Aswad, 2005. Antifeedant and growth inhibitory effects of tetranortriterpenoids isolated from three meliaceous species on the Cotton Leafworm, Spodoptera littoralis (Boisd.). Journal of Applied Sciences Research, 1(2): 234-241.
3. Anonymous, 2014. Assessment report of Fresh fruit-vegetable association. Overall in Turkey (2012/2013 January- December Period). Mediterranean Exporter Association General Secretariat, Report date: 01.01.2014.
4. Biondi, A., V. Mommaerts, G. Smagghe, E. Viňuela, L. Zappalà & N. Desneux, 2012. The non-target impact of spinosyns on beneficial arthropods. Pest Management Science, 68: 1523-1536.
5. Braham, M., H. Glida-Gnidez & L. Hajji, 2012. Management of the tomato borer, Tuta absoluta in Tunisia with novel insecticides and plant extracts. OEPP/EPPO Bulletin, 42(2): 291-296.
6. Dağlı, F., C. İkten, E. Sert & E. Bölücek, 2012. Susceptibility of tomato borer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) populations from Turkey to 7 different insecticides in laboratory bioassay. OEPP/EPPO Bulletin, 42(2): 305-311.
7. Durmuşoğlu, E., A. Hatipoğlu, & H. Balcı, 2011. Efficiency of some plant extracts against Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae) under laboratory conditions. Turkish Journal of Entomology, 35(4): 651-663.
8. EPPO, 2005. European and Mediterranean Plant Protection Organization Data sheets on quarantine pests. OEPP/EPPO Bulletin, 35: 434-435.

9. EPPO, 2009. Tuta absoluta found on Phaseolus vulgaris in Sicilia (2009/154). EPPO Reporting Services 8(154). (Web page: http://archives.eppo.int/EPPOReporting/2009/Rse-0908.pdf) (Access Date: 16.05.2014).
10. EPPO, 2010. First record of Tuta absoluta in Turkey (2010/208). EPPO Reporting Services 11(208). (Web page: http://archives.eppo.int/EPPOReporting/2010/Rse-1011.pdf) (Access Date: 15.05.2014).
11. Erdoğan, P., 2013. Properties of insecticides obteined from Azadirachta indica A. Juss and Melia azedarach L. and effect on pests. Karaelmas Science and Engineering Journal, 3(2): 14-25.
12. Gacemi, A. & Y. Guenaoui, 2012. Efficacy of emamectin benzoate on Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) infesting a protected tomato crop in Algeria. Academic Journal of Entomology, 5 (1): 37-40.
13. García-Marí, F. & J.C. Espul, 1982. Bioecology of the tomato moth (Scrobipalpula absoluta) in Mendoza, Argentine Republic. Revista de Investigaciones Agropecuarias, 17: 135-146.
14. Gates Clarke, J.F., 1962. New species of microlepidoptera from Japan. Entomological News Philadelphia, 73: 91-102 (Web page: http://biostor.org/reference/84584) (Access Date: 20.05.2014).
15. Ghosh, A., M. Chatterjee & A. Roy, 2010. Bio-efficacy of spinosad against tomato fruit borer (Helicoverpa armigera Hub.) (Lepidoptera: Noctuidae) and its natural enemies. Journal of Horticulture and Forestry, 2(5): 108-111.
16. Gökçe, A., R. Isaacs & M.E. Whalon, 2012. Dose–response relationships for the antifeedant effects of Humulus lupulus extracts against larvae and adults of the Colorado potato beetle. Pest Management Science, 68: 476- 481.
17. Hafsi, A., K. Abbes, B. Chermiti & B. Nasraoui, 2012. Response of the tomato miner Tuta absoluta (Lepidoptera: Gelechiidae) to thirteen insecticides in semi-natural conditions in Tunisia. OEPP/EPPO Bulletin, 42(2): 312- 316.
18. Hanafy, H.E.M. & W. El-Sayed, 2013. Efficacy of bio- and chemical insecticides in the control of Tuta absoluta (Meyrick) and Helicoverpa armigera (Hubner) infesting tomato plants. Australian Journal of Basic and Applied Sciences, 7(2): 943-948.
19. Isman, M.B., 1993. Growth inhibitory and antifeedant effects of azadirachtin on six noctuids of regional economic importance. Pest Management Science, 38(1): 57-63.
20. Karman, M., 1971. General Information on Plant Protection Researches, The establishment of experiments and assessment principles. Turkish Ministry of Agriculture, Career Books Series, Directorate of Plant Protection Central Research Institute, Regional Plant Protection Research Station, Bornova-Izmir, 279 pp.
21. Keçeci, M., 2010. The tomato leaf miner [Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae)]. Journal of Voice of Agriculture, 26: 9-12.
22. Kılıç, T., 2010. First record of Tuta absoluta in Turkey. Phytoparasitica, 38: 243-244.
23. Lietti, M.M.M., E. Botto & R.A. Alzogaray, 2005. Insecticide resistance in Argentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Neotropical Entomology, 34(1):113-119.
24. Liu, X., M. Chen, H.L. Collins, D. Onstad, R. Roush, Q. Zhang & A.M. Shelton, 2012. Effect of insecticides and Plutella xylostella (Lepidoptera: Plutellidae) genotype on a predator and parasitoid and implications for the evolution of insecticide resistance. Journal of Economic Entomology, 105(2): 354-362.
25. Lopez, J.A., F. Amor, P. Bengochea, P. Medina, F. Budia & E. Viñuela, 2011. Toxicity of emamectin benzoate to adults of Nesidiocoris tenuis Reuter, Macrolophus pygmaeus (Rambur) (Heteroptera: Miridae) and Diglyphus isaea Walker (Hymenoptera: Eulophidae) on tomato plants. Spanish Journal of Agricultural Research, 9(2): 617-622.
26. Moussa, S., A. Sharma, F. Baiomy & F.E. El-Adl, 2013. The Status of Tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in Egypt and potential effective pesticides. Academic Journal of Entomology, 6(3): 110-115.
27. Muniappan, R., 2014. Tuta absoluta: the Tomato leafminer. http://www.coraf.org/documents/ateliers/2013-05/tuta- absoluta/Tuta-absoluta-Presentation.pdf (Access Date: 20.05.2014).
28. Nannini, M., F. Foddi, G. Murgia, R. Pisci & F. Sanna, 2011. Insecticide efficacy trials for management of the tomato borer Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), a new tomato pest in Sardinia (Italy). Acta Horticulture, 917: 47-53.
29. Santos, A.C. Dos, R.C.O. de F. Bueno, S.S. Vieira & A. de F. Bueno, 2011. Efficacy of insecticides on Tuta absoluta (Meyrick) and other pests in pole tomato. Bioassay, 6(4):1-5.
30. Schoenly, K.G., M.B. Cohen, A.T. Barrion, W. Zhang, B. Gaolach & V.D. Viajante, 2003. Effects of Bacillus thuringiensis on non-target herbivore and natural enemy assemblages in tropical irrigated rice. Environmental Biosafety Research, 3:181-206.
31. Siqueira, H.A.A., R.N.C. Guedes & M.C. Picanco, 2000. Insecticide resistance in populations of Tuta absoluta. Agricultural and Forest Entomology, 2:147-153.
32. Siqueira, H.A.A., R.N.C. Guedes, D.B. Fragoso & L.C. Magalhaes, 2001. Abamectin resistance and synergism in Brazilian populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). International Journal of Pest Management, 47:247-251.
33. USDA, 2011a. New Pest Response Guidelines: Tomato Leafminer (Tuta absoluta). The United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service, Cooperating State Departments of Agriculture. 5/2011-01.
34. USDA, 2011b. Federal Import Quarantine Order for Host Materials of Tomato Leafminer, Tuta absoluta (Meyrick). SPRO# DA-2011-12. United States Department of Agriculture, Plant Protection and Quarantine. (Web page: http://www.aphis.usda.gov/import_export/plants/plant_imports/federal_order/downloads/2011/Tuta%20absolut a5-5-2011.pdf) (Access Date: 15.02.2014).
35. Tillman, P.G. & J.E. Moonroney, 2000. Effect of selected insecticides on the natural enemies Coleomegilla maculata (DeGeer) and Hippodamia convergens (Coleoptera: Coccinellidae), Geocoris punctipes (Hemiptera: Lygaeidae), and Bracon mellitor, Cardiochiles nigriceps, and Cotesia marginiventris (Hymenoptera: Braconidae) in cotton. Journal of Economic Entomology, 93(6): 1638-1643.
36. Tunca, H., N. Kilinçer & C. Özkan, 2012. Side-effects of some botanical insecticides and extracts on the parasitoid, Venturia canescens (Grav.) (Hymenoptera: Ichneumonidae). Turkish Journal of Entomology, 36(2): 205-214.
37. Turanlı, F., E. Gümüş & B. Güzel, 2012. Studies on the efficacies of combinations of Bacillus thuringiensis with neem extracts. Turkish Journal of Entomology, 36(3): 433-439.
38. TÜİK, 2013. Crop Production, 2013. Turkish Statistical Institute News Releases, No 13656 (27 December 2013). (Web page: http://www.turkstat.gov.tr/PreHaberBultenleri.do?id=13656) (Access Date: 20.05.2014).
39. Viñuela, E., A. Adan, G. Smagghe, M. Gonzalez, Ma.P. Medina, F. Budia, H. Vogt & P. Del Estal, 2000. Laboratory effects of ingestion of azadirachtin by two pests (Ceratitis capitata and Spodoptera exigua) and three natural enemies (Chrysoperla carnea , Opius concolor and Podisus maculiventris). Biocontrol Science and Technology, 10: 165-177.