Kültürü Yapılan Deniz Balıklarında Karaciğer Yağlanması ve Bakteriyel Koenfeksiyon

Öz

Bu çalışmada rutin mikrobiyolojik, hematolojik, makroskopik ve histopatolojik incelemeler için 400-450 gr ağırlığında çipura ve levrek balıkları örneklenmiştir. Örnekler morfolojik olarak sağlıklı görünen ancak artan yem dönüşüm oranı, hasat sırasında şoklanma (soğuk şoku) sorunu gösteren ve hareketlerde yavaşlık gibi semptom gösteren balıklardan örnekler alınmıştır. Gram boyama, hareketlilik, oksidaz-katalaz aktivitesi ve O/F fermentasyonu gibi rutin bakteriyolojik analizler ve API test kitleri kullanılarak temel mikrobiyolojik analizlerle bakterilerin mikrobiyolojik teşhisleri yapılmıştır. Ön teşhisi yapılan türler16S rRNA gen bölgesi sekans analizi ile tür bazında identifiye edilmiştir. Serum biyokimyasal ve hematolojik değerleri, Vetscan® VS2 cihazı ve kan yayma frotileri kullanılarak analiz edilmiştir. Hastalıktan etkilenen iç organların incelenmesi için rutin histopatolojik incelemeler yapılmıştır. Hepatik yağ birikimi ve nekroz görünen en belirgin bulgulardan olmuştur ve karaciğer enzimlerinde önemli değişikliklerin karaciğer hasarıyla ilişkili olduğu tespit edilmiştir. Etkilenen balıklardan çoklu organ yetmezliği ve hematolojik bozukluklar nedeniyle başta Vibrio alginolyticus olmak üzere Alteromonas ve Pseudoalteromonas türleri izole edilmiş ve hastalık belirtileri gösteren balıkların bakteriyel koenfeksiyon taşıdığı tespit edilmiştir.

Anahtar Kelimeler

• Deniz balıklarında hematoloji
• hepatik lipidosis
• V. alginolyticus
• yağlı karaciğer sendromu

Fatty Liver Disease and Bacterial Co-Infection in Cultured Marine Fish

Öz

In the present study, we sampled sea bream and sea bass, weighing 400-450g, for routine microbiological, hematological, macroscopical and histopathological examinations. Samples were taken from seemingly healthy fish, which displayed increased feed conversion rates, signs of cold shock and slow movement. Routine bacteriological analyses included basic microbiological analyses for oxidase and catalase activity, Gram staining and O/F fermentation using API test kits. Furthermore, 16S rRNA gene sequencing was performed for identification. Serum biochemical and hematological values were determined using a Vetscan®VS2 analyzer and blood smears. Internal organs were examined by routine histopathological techniques. Hepatic fat accumulation and necrosis were noted, and liver damage was observed to be associated with significant alterations in liver enzymes. Due to multi-organ dysfunction and serious hematological disorders, primarily Vibrio alginolyticus also Alteromonas and Pseudoalteromonas species were isolated from the affected fish and co-infections were detected.

Anahtar Kelimeler

• Fatty liver syndrome
• hematology in marine fish
• hepatic lipidosis
• V. alginolyticus

Kaynakça

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