Endothelial Cell Specific Molecule-1 (Endocan) Levels in Dogs with Parvoviral Enteritis

Öz

Endocan or Endothelial Cell Specific Molecule-1 (ESM-1) has been proven to play a key role in the regula-tion of processes such as cell adhesion, inflammation and tumor development. In this study, we aimed to investigate the role of Endocan as a potential inflammatory biomarker in dogs with Parvoviral Enteritis (PVE) due to increased Endocan levels in the presence of proinflammatory and proangiogenic molecules. The study included 40 dogs (24 ma-les, 16 females), 6-20 weeks old, of different breeds, with diarrhea (bloody/bloodless), weakness and canine parvovirus (CPV) antigen positive dogs. The control group consisted of 20 healthy dogs (12 males, 8 females). Endocan, IL-6 and CRP levels in serum samples of all dogs were analyzed by sandwich ELISA method. Mean Endocan levels were signi-ficantly higher in dogs with PVE (68.07 ng/L; 17.30-115.55) compared to the healthy group (11.92 ng/L; 10.32-13.58) (P<0.001). Both mean CRP level (20.87±6.34 mg/L) and mean IL-6 levels (2.32±0.84 pg/ml) in the patient group were statistically significantly (P<0.01) higher than the healthy group (2.24±0.66 mg/L and 1.07±0.61 pg/ml). In addition, due to the positive correlations between Endocan and CRP levels and IL-6 values, Endocan was considered to be a syste-mic component of the inflammatory process. In conclusion, findings supporting that Endocan levels in dogs with parvo-viral enteritis is an important biomarker in patients were obtained.

Anahtar Kelimeler

• Biomarker
• dog
• endocan
• parvoviral enteritis

Parvoviral Enteristisli Köpeklerde Endotel Hücre Spesifik Molekül-1 (Endocan) Düzeyleri

Öz

Endocan veya Endotelyal Hücre Spesifik Molekül-1 (ESM-1), hücre adezyonu, inflamasyon ve tümör gelişimi gibi süreçlerin düzenlenmesinde anahtar bir rol oynadığı kanıtlanmıştır. Bu çalışmada proinflamatuar ve proanjiyojenik moleküllerin varlığında Endocan düzeylerindeki artış nedeniyle Parvoviral Enteritis’li (PVE) köpeklerde potansiyel yan-gısal biyobelirteç rolünün araştırılması amaçlandı. Çalışmaya 6-20 haftalık, farklı ırklarda, 40 adet (24 erkek, 16 dişi) ishal (kanlı/kansız), halsizlik şikâyeti bulunan ve köpek parvovirüs (CPV) antijen pozitif köpek dâhil edildi. Kontrol gru-bunu; 20 adet sağlıklı köpek (12 erkek, 8 dişi) oluşturdu. Tüm köpeklerin serum örneklerinde; Endocan, IL-6 ve CRP düzeyleri sandviç ELISA metodu ile analiz edildi. Ortalama Endocan düzeylerinin PVE’li köpeklerde (68.07 ng/L; 17.30-115.55) sağlıklı gruba göre (11.92 ng/L; 10.32-13.58) istatistiksel açıdan (P<0.001) anlamlı düzeyde yüksek olduğu saptandı. Hasta grubundaki hem ortalama CRP düzeyi (20.87±6.34 mg/L), hem de ortalama IL-6 düzeyleri (2.32±0.84 pg/ml), sağlıklılardan (2.24±0.66 mg/L ve 1.07±0.61 pg/ml) istatistiksel açıdan önemli oranda (P<0.01) yüksekti. Ayrıca Endocan ile CRP düzeyleri ve IL-6 değerleri arasındaki pozitif korelasyonlar nedeni ile Endocan’ın yangısal sürecin sistemik bir bileşeni olduğu değerlendirildi. Sonuçta PVE’li köpeklerde Endocan düzeylerinin hastalarda önemli bir bi-yobelirteç olduğunu destekleyen bulgular elde edildi.

Anahtar Kelimeler

• Biyobelirteç
• endocan
• köpek
• parvoviral enteritis

Kaynakça

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