Ratlarda Pentaklorofenol Zehirlenmesinde Nar Çekirdeği Yağının Lipid Peroksidasyonu ve Biyokimyasal Parametrelere Etkileri

Öz

Çalışma Sprague-Dawley ırkı erkek sıçanlarda yapıldı. Çalışmada ilki kontrol olmak üzere 4 grup oluşturuldu. İkinci gruba 0.15ml/kg dozunda nar çekirdeği yağı (NÇY), üçüncü gruba 40mg/kg dozunda pentaklorofenol (PCP) ve dördüncü gruba 40mg/kg dozunda PCP+0.15ml/kg dozunda NÇY 28 gün boyunca gavaj ile uygulandı. Deneme sonunda eritrositte hemoglobin, katalaz ve glutasyon peroksidaz, nitrik oksit, malondialdehit, süperoksit dismutaz parametreleri; serumda 8-hidroksi-2-deoksi guanosin (8-OH-dG) ve biyokimyasal parametreleri incelendi. Sonuç olarak, bu çalışmada sıçanlara oral yoldan gavaj ile 40mg/kg uygulanan PCP’nin karaciğer hasarına, lipid peroksidasyonuna ve antioksidan enzim etkinliklerinde azalmaya yol açtığı; NÇY’nin PCP’nin istenmeyen etkileri üzerine koruyucu rolü olduğu belirlendi.

Anahtar Kelimeler

• Lipid peroksidasyon,nar çekirdeği yağı,pentaklorofenol,rat

Effects of Pomegranate Seed Oil on Pentachlorophenol Toxicity Lipid Peroxidation and Some Biochemical Parameters in Rats

Öz

In the study, Sprague-Dawley male rats were used and four groups were formed. The first group was held

as the control group. The second group was given 0.15 ml/kg pomegranate seed oil (PSO), the third group was given

40mg/kg pentachlorphenol (PCP) and, the forth group was given 40mg/kg PCP+0.15 ml/kg PSO during 28 days

as specified into stomach by gavages. At the end of the experiment, erythrocyte haemoglobin, catalase, glutathione

peroxidase, nitric oxide, blood serum 8-hidroksi-2-deoksi guanosin (8-OH-dG) and biochemical parameters were

analysed. As a result, 40mg/kg PCP applied by gavage, caused liver damage, lipid peroxidation and decreased the

anti-oxidant enzyme activity and 0.15ml/kg PSD applied for 28 days, had no negative effect on protein, oil and carbonhidrate

metabolism, had no toxic effect on liver and had no effect on lipid peroxidation. Overall, it’s determined

that pomegranate seed oil prevents the negative effects of the pentachlorophenol.

Anahtar Kelimeler

• Lipid peroxidation,pentachlorophenol,pomegranate seed oil,rat

Kaynakça

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