Investigation of the Relationship between FABP4, NR1H3 and SCD Genes and Some Milk Yield Traits in Holstein Cattle

Öz

The aim of this study was to investigate the relationship between genes that encode fatty acid-binding protein 4 (FABP4), nuclear receptor subfamily 1, group H, member 3, (NR1H3) and stearoyl-CoA desaturase (SCD) proteins and some milk yield traits in Holstein cattle. A total of 166 Holstein dairy cows were used for the study. Genotyping was performed by polymerase chain reaction and restriction length polymorphism (PCR-RFLP) method. The frequencies of FABP4-G (82%), NR1H3-G (96%) and SCD-C (73%) alleles were found to be high in the examined Holstein cows. According to chi-square test results, the investigated Holstein cows were in Hardy-Weinberg (HW) equilibrium for the FABP4 and SCD genes while significant deviation was observed from HW equilibrium for the NR1H3 gene. Regression analyses revealed that NR1H3 genotypes were associated with milk fat (P<0.05), SCD genotypes were associated with dry matter without fat (P<0.05) and FABP4 genotypes were associated with milk density (P<0.01). Taken together, our results indicate that investigated SNPs may be used in the selection programs related with the milk composition traits in dairy cattle.

Anahtar Kelimeler

• Genotype frequency,Holstein,milk yield traits,polymorphism

Holstein Irkı Sığırlarda FABP4, NR1H3 ve SCD Genleri ile Bazı Süt Verim Özellikleri Arasındaki İlişkilerin Araştırılması

Öz

Bu çalışmada Holstein ırkı sığırlarda yağ asit bağlayıcı protein 4 (FABP4), nükleer reseptör alt ailesi 1, grup H, üye 3 (NR1H3) ve stearoyl-CoA desaturaz (SCD) proteinlerini kodlayan genler ile bazı süt verim özellikleri arasındaki ilişkilerin araştırılması amaçlanmıştır. Çalışmanın hayvan materyalini 166 baş sağmal Holstein inek oluşturmuştur. İncelenen hayvanlar polimeraz zincir reaksiyonu-restriksiyon parçacık uzunluk polimorfizmi (PCR-RFLP) yöntemi ile yukarıda belirtilen genler yönünden genotiplendirilmiştir. İncelenen örneklerde FABP4 geni için G allel frekansının (%82), NR1H3 geni için G allel frekansının (%96) ve SCD geni için ise C allel frekansının (%73) en yüksek olduğu görülmüştür. Yapılan Hardy-Weinberg (HW) ki-kare analizleri sonucunda incelenen Holstein ineklerin FABP4 ve SCD genleri yönünden HW dengesinde oldukları, NR1H3 geni yönünden ise HW dengesinde olmadıkları görülmüştür. Çalışma sonunda, sütteki yağ oranı yönünden NR1H3 genotipleri arasındaki farklılık (P<0.05), yağsız kuru madde oranı yönünden SCD genotipleri arasındaki farklılık (P<0.05) ve süt yoğunluğu yönünden FABP4 genotipleri arasındaki farklılık (P<0.01) istatistiki olarak önemli bulunmuştur. Sonuç olarak incelenen genlerin süt kompozisyonu için yapılacak seleksiyon çalışmalarında kullanılabileceği düşünülmüştür.

Anahtar Kelimeler

• Genotip frekansı,Holstein,polimorfizm,süt verim özellikleri

Kaynakça

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