Biosynthesis of Silver@Graphene Oxide Nanocomposite and Determination of It’s Antimicrobial Activity

Yıl 2020, , 126 - 130, 27.07.2020

Fatih Doğan Koca

https://doi.org/10.32707/ercivet.760738

Öz

Nanoparticles (NPs), which are widely used in the industry, can be modified with materials such as graphene and chitosan to gain new properties in terms of their hydrophilicity and oxidation capacity. In this study, unlike Ag NP synthesis via Syzygium aromaticum extract, silver graphene oxide nanocomposites (Ag@GO NC) were synthesized different from Ag NP synthesis via Syzygium aromaticum extract and characterized. The antimicrobial activity of Ag@GO NC against to Staphylococcus aureus was tested. As a result of characterization studies, S. aromaticum based Ag NPs have short-term stability (-16.1 mV). Dynamic light scattering (DLS) analysis showed effective diameter of NPs is approximately 327 nm. X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FT-IR) analysis showed that the crystalinity of NPs and the presence of functional groups in NP synthesis process, respectively. Ag@GO NCs were recorded by Scanning Electron Microscopy (SEM). Ag@GO NCs have been reported to have antimicrobial properties against S. aureus strain. The study is original and innovative in terms of biosynthesis and determining the antimicrobial activities of Ag@GO NCs with S. aromaticum bud extract. It is thought that the data obtained in this study will shed light on the studies in biomedical fields by modifying NPs

Anahtar Kelimeler

Antimicrobial activity, green sytnhesis, silver@graphene oxide

Gümüş@Grafen Oksit Nanokompozitin Biyolojik Sentezi ve Antimikrobiyal Aktivitesinin Belirlenmesi

Öz

Endüstride yaygın bir şekilde kullanılan nanopartiküller (NP’ler) grafen ve kitosan gibi maddelerle modifiye edilerek hidrofilitesi ve oksidasyon kapasitesinin artması yönüyle yeni özellikler kazanabilmektedir. Bu çalışmada Syzygium aromaticum özütü ile AgNP sentezinden farklı olarak, gümüş grafen oksit nanokompozitler (Ag@GO NK) sentezlenerek karakterizasyonu değerlendirilmiştir. Elde edilen Ag@GO NK’nin Staphylococcus aureus türüne karşı antimikrobiyal aktivitesi test edilmiştir. Karakterizasyon çalışmaları sonucunda, S. aromaticum tabanlı Ag NP’lerin kısa süreli stabiliteye sahip olduğu (-16.1 mV) belirlenmiştir. Dinamik ışık saçılımı (DLS) analizi ile NP’lerin yaklaşık 327 nm etkin çapa sahip olduğu tespit edilmiştir. Sırayla X Işınları Toz Difraksiyon (XRD) ve Fourier Dönüşümlü Kızılötesi Spektroskopisi (FT-IR) analizi ile NP’lerin kristal yapısı ve NP sentezinde işlevsel özelliğe sahip fonksiyonel grupların varlığı gösterilmiştir. Taramalı Elektron Mikroskobu (SEM) ile Ag@GO NK’lerin morfolojileri incelenmiştir. Ag@GO NK’lerin S. aureus suşuna karşı antimikrobiyal özelliğe sahip olduğu bildirilmiştir. Çalışma, S. aromaticum tomurcuk özütü ile Ag@GO NK’lerin bioysentezi ve antimikrobiyal aktivitelerinin belirlenmesi açısından özgün ve yenilikçidir. Çalışmada elde edilen verilerin NP’lerin modifiye edilerek biyomedikal çalışmalara ışık tutacağı düşünülmektedir.

Anahtar Kelimeler

Antimikrobiyal aktivite, biyolojik sentez, gümüş@grafen oksit

Kaynakça

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