Kayseri Şehir Merkezinde Kasap ve Marketlerde Satışa Sunulan Sığır Etlerinde PCR Yöntemi ile Cinsiyet Tayini*

Yıl 2015, Cilt: 12 Sayı: 2, 75 - 79, 01.07.2015

Korhan Arslan Bilal Akyüz Esma Gamze Aksel

Öz

Et büyüme ve gelişmenin tamamlanabilmesi için gerekli temel protein kaynağıdır. Et türleri içinde sığır eti tüketimi ülkemizde
önemli bir yere sahiptir. Ette kalite standartlarından biri dişi ve erkek sığır etinin ayırımıdır. Erkek sığırlardan elde edilen
etler dişilerden elde edilenlere göre daha kaliteli ve satış fiyatları daha yüksektir. Bu da kasaplık olarak satışa sunulan etlerde
cinsiyet tayinini önemli kılmaktadır. Cinsiyetin belirlenebilmesi için farklı laboratuvar yöntemleri geliştirilmiştir. Bu çalışmada
polimeraz zincir reaksiyonu (PCR) ile Y kromozomuna özgü genleri ayırt eden iki farklı primer seti kullanılarak, kasaplık olarak
satışa sunulan sığır etlerinde cinsiyet tayininin araştırılması amaçlanmıştır. Yapılan çalışmada elde edilen sonuçlara göre
kullanılan 30 adet et örneğinden 15’inin dişi 15’inin erkek cinsiyete ait bant görüntüleri verdiği saptanmıştır

Anahtar Kelimeler

Cinsiyet, PCR, sığır eti

Determination of Gender by using PCR Method on Cattle Meats Sold in Butcher’s and Markets in Kayseri City Center

Öz

Meat is an essential protein source required by the body for growth and development. Cattle meat has an important
area in our country. Determination of gender is one of the quality standards for meat. The quality of meat obtained
from male cattles is hingher than that of female ones so the prices of male cattle meat is higher than female cattle meat. A
number of methods have been developed for gender determination on meat. In this study, Y chromosome-specific genes that
distinguish two different primer sets on the availability of gender determination in cattle meat was aimed to investigate with
the polymerase chain reaction (PCR). PCR results were obtained on meat samples (30); 15 female, 15 male genders were
determined through gel-electrophoresis images.

Anahtar Kelimeler

Gender, PCR, cattle meat

Kaynakça

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