Şap Hastalıklı Besi Sığırlarında Pratik Kardiyak Troponin I Kit Sonuçları ve Kardiyak Troponin I Düzeyleri

Yıl 2015, Cilt: 12 Sayı: 3, 163 - 170, 01.10.2015

Halim Akın Vehbi Güneş , İlknur Karaca Bekdik

Öz

 Bu çalışmanın amacı, immünokromatografik test kitleri ile şap hastalığı (FMD) bulunan besi sığırlarında cTn-I’nın belirlenmesi
ve miyokard dejenerasyonlarında söz konusu parametrenin düzeylerini belirlemekti. Bu amaçla akut FMD’li (I. Grup)
ortalama 12.4±0.5 aylık yaşta 69 adet ve klinik olarak sağlıklı (II. Grup) ortalama 10.4±0.6 aylık yaşta 20 adet sığır kullanıldı.
Her iki grupta da kreatin kinaz-MB (CK-MB) ve laktat dehidrogenaz (LDH ) aktiviteleri, cTn-I düzeyleri ve cTn-I varlığı belirlendi.
FMD’li sığırlarda ortalama CK-MB ve LDH aktiviteleri, cTn-I düzeyi sırasıyla ortalama 795.1±70.8 U/L, 1305.9±51.4 U/L
ve 3.6±0.3 ng/ml olarak bulundu. Aynı parametreler II.Grupta sırasıyla ortalama 306±20.5 U/L, 1084.7±43.2 U/L ve 1.1±0.02
ng/ml olduğu belirlendi. Bu parametreler I. Grupta, kontrol grubuna göre istatistiksel olarak yüksek bulundu (p<0.05). Pratik
cTn-I kitlerinin kalp kası dejenerasyonunun tanısında duyarlılık ve seçicilik oranları sırasıyla %100 ve %76.92 olarak bulundu.
FMD’li sığırlardan yalnızca beşinde kardiyak Tn-I testleri pozitif sonuç verdi. Ancak bu pratik testler sağlıklı sığırlardan toplanan
kan örneklerinde negatif idi. Makroskobik bulgular, serum cTn-I düzeyleri ve pozitif cTn-I sonuçları Şap hastalığına bağlı
miyokard dejenerasyonu olan vakalarla uyumlu bulundu. Sonuç olarak, hem kantitatif cTn-I analizleri hem de kalitatif cTn-I
varlığı FMD’li sığırlarda ölüm oranının belirlenmesi için duyarlı ve spesifik bir belirleyici olabileceği ve sığırlarda gelişebilecek
kalp kası hastalıklarını belirlemek için kullanılabileceği gösterilmiştir.

Anahtar Kelimeler

CK-MB, cTn-I, LDH, sığır, şap hastalığı

Practice Cardiac Troponin I Kit Expressions and Concentrations of Cardiac Troponin I in Beef Cattle with Foot and Mouth Disease*

Öz

 The aim of this study was to determine the expression of cTn-I with immunochromatographic strip kits, the concentrations of serum cTn-I in myocardial degeneration due to foot and mouth disease (FMD) with these parameters in beef cattle. For this purpose sixtynine cattle with acute FMD (Group I), mean age 12.4±0.5 months old and 20 clinically healthy cattle (Group II), mean age 10.4±0.6 months old were used. Creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) activities, cTn-I concentrations and expression of cTn-I were determined in both groups. Mean CK-MB, LDH activities and cTn-I concentration were found as 795.1±70.8 U/L, 1305.9±51.4 U/L and 3.6±0.3 ng/ml respectively in cattle with FMD. The mean of the same parameters were 306±20.5 U/L, 1084.7±43.2 U/L and 1.1±0.02 ng/ml in Group II, respectively. These parameters in Group I were statistically higher than those of control group (p<0.05). The sensitivity and specificity of cTn-I kits for the diagnosis myocardial degeneration were 100% and 76.9%, respectively. Cardiac Tn-I tests were positive in the only five cattle with FMD, but these tests were negative in blood samples collected from healthy cattle. Macroscobic findings, serum cTn-I concentrations and positive cTn-I results were correlated with FMD cases suffering from myocardial degeneration. In conclusion, both quantitative cTn-I analysess and qualitative cTn-I expressions may be sensitive and specific marker for the determination of mortality rate in cattle with FMD, and can be used to predict the future cardiac disorders in cattle.

Anahtar Kelimeler

Cattle, CK-MB, cTn-I, FMD, LDH

Kaynakça

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