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In vitro Effects of Chitosan on the Survival of Listeria monocytogenes

Yıl 2016, Cilt 13, Sayı 1, 11 - 18, 01.08.2016

Öz

The aim of this study is to evaluate the in vitro effects of different molecular weights of chitosan on the growth of three Listeria monocytogenes strains isolated from mayonnaise-based salad and of a L. monocytogenes reference strain (ATCC 7644). All L. monocytogenes isolates were numerically adjusted to 2.0x105 cfu/mL and were treated with 0.1% chitosan solutions that had been prepared by dissolving low, medium and high molecular weight chitosan in 1% acetic acid at different pH values (4.0, 4.5 and 5.0) in vitro. All L. monocytogenes isolates were inhibited 3 log levels following 24 h incubation in a low molecular weight chitosan solution at pH 4.0, whereas 2 log levels of inhibition were observed for medium and high molecular weight chitosan solutions. The effect of different molecular weighted chitosan solutions and pH on L. monocytogenes strains in vitro was found to be statistically significant.

Kaynakça

  • 1. Agulló E, Rodríguez MS, Ramos V, Albertengo L. Present and future role of chitin and chitosan in Food. Macromol Biosci, 2003; 3 (1): 521-30.
  • 2. Anonymus. Microbiological Reference Criteria for Food. Food Administration Manual. Version 2.0. http://www.nzfsa.govt.nz/processed-food-retail-sale/fact-sheets/nzmicro-ref.pdf. Access Date: 01.01.2009.
  • 3. Anonymus. Commission Regulation (EC) 2073/2005 on Microbiological Criteria for Foodstuffs: Official Journal of European Union.
  • 4. Anonymus. Turkish Food Codex. Microbiological Criteria Notification No2009/6, Official Gazette, 06.02.2009-27133, http://www.kkgm.gov.tr/TGK/Teblig/20096.html. Access Date: 01.01.2009.
  • 5. Aznar R, Alarcon B. PCR detection of Listeria monocytogenes: A study of multiple factors affecting sensitivity. J Appl Microbiol, 2003; 95(1): 958-66.
  • 6. Bautista-Baños S, Hernández-Lauzardo AN, Velázquez-del Vale MG, Hernández-López M, Ait Barka E, Bosquez-Molina E, Wilson CL. Chitosan as a potential natural compound to control pre and post harvest diseases of horticultural commodities: Review. Crop Protect 2006; 25:108-18.
  • 7. Bohnert M, Dilasser F, Dalet C, Mengaud J, Cossart P. Use of specific oligonucleotides for Direct enumeration of Listeria monocytogenes in food samples by colony hybridization and rapid detection by PCR. Res Microbiol 1992; 143: 271-80.
  • 8. Devlieghere F, Vermeulen A, Debevere J. Chitosan. Antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol 2004; 21(6): 703-14.
  • 9. Faleiro ML, Andrew PW, Power D. Stress response of Listeria monocytogenes isolated from cheese and other foods. Int J Food Microbiol, 2003; 84(2): 207-16.
  • 10. FDA. US Food and Drug Administration. Center for Food Safety and Approval, GRAS Notices Received in 2001, http:/vm.cfsan.fda.gov. Access Date: 01.01.2009.
  • 11. Ferreira A, Sue D, O’Byrne CP, Boor KJ. Role of Listeria monocytogenes sigma (B) in survival of lethal acidic conditions and in the acquired acid tolerance response. Appl Environ Microbiol 2003; 69(5): 2692-8.
  • 12. Gahan CG, O’Mahony J, Hill C. Characterization of the groESL operon in Listeria monocytogenes: utilization of two reporter systems (gfp and hly) for evaluating in vivo expression. Infect Immun 2001; 69: 3924-32.
  • 13. Hanawa T, Fuduka M, Kawakami H, Hirano H, Kamiya S, Yamamoto T. The Listeria monocytogenes DnaK chaperone is required for stress tolerance and efficient phagocytosis with macrophages. Cell Stres Chap, 1999; 4(2): 118-28.
  • 14. Hitchins AD. Bacteriological Analytical Manual FDA: Listeria monocytogenes. 7 Edition. Arlington, AOAC International, 1998;p:10-12
  • 15. Jay JM, Loessner MJ, Golden DA. Foodborne listeriosis. Modern Food Microbiology. 7th ed. New York USA, Springer Science and Business Media, 2005; p. 591-611.
  • 16. Kallipolitis BH, Ingmerb H. Listeria monocytogenes response regulators important for stress tolerance and pathogenesis. FEMS Microbiol Lett 2001; 204(1): 111-5.
  • 17. Liu D. Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol 2006; 55(1): 645-59.
  • 18. Liu H, Du Y, Wang X, Sun L. Chitosan kills bacteria through cell membrane damage. Int J Food Microbiol 2004; 95(1): 147-55.
  • 19. Maheti NV, Kumar R. A review of chitin and chitosan applications. React Funct Polym 2000; 46(1): 1-27.
  • 20. McClain D, Lee WH. Development of USDA/FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J Assoc Offic Anal Chem 1988; 71(1): 660-4.
  • 21. Moller H, Grelier S, Pardon P, Coma V. Antimicrobial and physicochemical properties of chitosan HPMC based films. J Agric Food Chem 2004; 52(1): 6585-91.
  • 22. No HK, Kim SH, Lee SH, Park NY, Prinyawiwatkul W. Stability and antibacterial activity of chitosan solutions affected by storage temperature and time. Carbohydr Polym 2006; 65(1): 174-8.
  • 23. No HK, Park NY, Lee SH, Meters SP. Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int J Food Microbiol 2002; 74(1): 65-72.
  • 24. Saggo S, Board R, Roller S. Chitosan inhibits growth of spoilage microorganisms in chilled pork products. Food Microbiol 2002; 19(1): 175-82.
  • 25. Sandford P. Chitosan: commercial uses and potential applications. Skjak-Braek, G., Anthosen, T.,Standford, P. (Eds.), Chitin and Chitosan. Sources, Chemistry, Biochemistry. In Physical Properties and Applications, London and New York, Elsevier Applied Science, 1989; pp. 51–69.
  • 26. Schlech WF. Foodborne listeriosis. Clin Infect Dis 2000; 31(1): 770-5.
  • 27. Shahidi F, Arachchi JKV, Jeon YJ. Food applications of chitin and chitosans. Trends Food Sci Tech 1999; 10(1): 37–51.
  • 28. Sudarshan NR, Hoover DG, Knorr D. Antibacterial action of chitosan. Food Biotechnol 1992; 6(3): 257-72.
  • 29. Uchida Y, Izume M, Ohtakara A, Preparation of chitosan oligomers with purified chitosanase and its application. in G. Skjak- Braek, T. Anthonsen, P. Sandford, Chitin and chitosan pp.373–382. 1989. London, Elsevier.
  • 30. Uyttendaele M, Busschaert P, Valero A, Geeraerd AH, Vermeulen A, Jacxsens L, Goh KK, De Loy A, Vanimpe JF, Devlieghere F. Prevalence and challenge tests of Listeria monocytogenes in Belgian produced and retailed mayonnaise-based deli-salads, cooked meat products and smoked fish between 2005 and 2007. Int J Food Microbiol 2009; 133(1): 94–104.
  • 31. Vartiainen J, Motion R, Kulonen H, Rätto M, Skyttä E, Ahvenaınen R. Chitosan-coated paper: Effects of nisin and different acids on the antimicrobial activity. J Appl Polym Sci 2004; 94(1): 986-93.
  • 32. Vialette M, Pinon A, Chasseignaux E, Lange M. Growths kinetics comparison of clinical and seafood Listeria monocytogenes isolates in acid and osmotic environment. Int J Food Microbiol 2003; 82(1): 121-31.
  • 33. Vlaemynck G, Lafarge V, Scotter S. Improvement of the detection of Listeria monocytogenes by the application of ALOA, a diagnostic, chromogenic isolation medium. J Appl Microbiol 2000; 88(1): 430-41.

Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi

Yıl 2016, Cilt 13, Sayı 1, 11 - 18, 01.08.2016

Öz

Bu çalışmanın amacı, farklı moleküler ağırlıktaki kitosan solusyonlarının mayonez bazlı salatalardan elde edilen üç Listeria monocytogenes saha izolatı ile L. monocytogenes (ATCC 7644) referans suşu üzerindeki etkilerini in vitro koşullarda değerlendirmekti. İn vitro koşullarda bütün L. monocytogenes izolatları sayısal olarak 2.0x105 cfu/mL’ye ayarlandıktan sonra farklı moleküler ağırlıktaki (düşük, orta ve yüksek) kitosanların %1’lik asetik asit içersinde çözündürülmek suretiyle farklı pH değerlerine (4.0, 4.5 ve 5.0) sahip % 0.1’lik solusyonları hazırlandı. Her bir izolat hazırlanan kitosan solusyonlarıyla muamele edildi. Düşük moleküler ağırlıklı ve pH değeri 4 olan kitosan solusyonuna maruz bırakılan bütün L. monocytogenes suşlarının 24 saat sonunda 3 log düzeyinde inhibe olduğu, orta ve yüksek moleküler ağırlıklı kitosan solusyonuna maruz bırakılanlarda ise 2 log düzeyinde bir inhibisyon şekillendiği gözlemlendi. Çalışmada in vitro koşullarda farklı moleküler ağırlıklı ve farklı pH değerine sahip kitosan solusyonlarının L. monocytogenes üzerine istatistiksel olarak farklı inhibitorik etkiler ortaya koyduğu belirlendi.

Kaynakça

  • 1. Agulló E, Rodríguez MS, Ramos V, Albertengo L. Present and future role of chitin and chitosan in Food. Macromol Biosci, 2003; 3 (1): 521-30.
  • 2. Anonymus. Microbiological Reference Criteria for Food. Food Administration Manual. Version 2.0. http://www.nzfsa.govt.nz/processed-food-retail-sale/fact-sheets/nzmicro-ref.pdf. Access Date: 01.01.2009.
  • 3. Anonymus. Commission Regulation (EC) 2073/2005 on Microbiological Criteria for Foodstuffs: Official Journal of European Union.
  • 4. Anonymus. Turkish Food Codex. Microbiological Criteria Notification No2009/6, Official Gazette, 06.02.2009-27133, http://www.kkgm.gov.tr/TGK/Teblig/20096.html. Access Date: 01.01.2009.
  • 5. Aznar R, Alarcon B. PCR detection of Listeria monocytogenes: A study of multiple factors affecting sensitivity. J Appl Microbiol, 2003; 95(1): 958-66.
  • 6. Bautista-Baños S, Hernández-Lauzardo AN, Velázquez-del Vale MG, Hernández-López M, Ait Barka E, Bosquez-Molina E, Wilson CL. Chitosan as a potential natural compound to control pre and post harvest diseases of horticultural commodities: Review. Crop Protect 2006; 25:108-18.
  • 7. Bohnert M, Dilasser F, Dalet C, Mengaud J, Cossart P. Use of specific oligonucleotides for Direct enumeration of Listeria monocytogenes in food samples by colony hybridization and rapid detection by PCR. Res Microbiol 1992; 143: 271-80.
  • 8. Devlieghere F, Vermeulen A, Debevere J. Chitosan. Antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol 2004; 21(6): 703-14.
  • 9. Faleiro ML, Andrew PW, Power D. Stress response of Listeria monocytogenes isolated from cheese and other foods. Int J Food Microbiol, 2003; 84(2): 207-16.
  • 10. FDA. US Food and Drug Administration. Center for Food Safety and Approval, GRAS Notices Received in 2001, http:/vm.cfsan.fda.gov. Access Date: 01.01.2009.
  • 11. Ferreira A, Sue D, O’Byrne CP, Boor KJ. Role of Listeria monocytogenes sigma (B) in survival of lethal acidic conditions and in the acquired acid tolerance response. Appl Environ Microbiol 2003; 69(5): 2692-8.
  • 12. Gahan CG, O’Mahony J, Hill C. Characterization of the groESL operon in Listeria monocytogenes: utilization of two reporter systems (gfp and hly) for evaluating in vivo expression. Infect Immun 2001; 69: 3924-32.
  • 13. Hanawa T, Fuduka M, Kawakami H, Hirano H, Kamiya S, Yamamoto T. The Listeria monocytogenes DnaK chaperone is required for stress tolerance and efficient phagocytosis with macrophages. Cell Stres Chap, 1999; 4(2): 118-28.
  • 14. Hitchins AD. Bacteriological Analytical Manual FDA: Listeria monocytogenes. 7 Edition. Arlington, AOAC International, 1998;p:10-12
  • 15. Jay JM, Loessner MJ, Golden DA. Foodborne listeriosis. Modern Food Microbiology. 7th ed. New York USA, Springer Science and Business Media, 2005; p. 591-611.
  • 16. Kallipolitis BH, Ingmerb H. Listeria monocytogenes response regulators important for stress tolerance and pathogenesis. FEMS Microbiol Lett 2001; 204(1): 111-5.
  • 17. Liu D. Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol 2006; 55(1): 645-59.
  • 18. Liu H, Du Y, Wang X, Sun L. Chitosan kills bacteria through cell membrane damage. Int J Food Microbiol 2004; 95(1): 147-55.
  • 19. Maheti NV, Kumar R. A review of chitin and chitosan applications. React Funct Polym 2000; 46(1): 1-27.
  • 20. McClain D, Lee WH. Development of USDA/FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J Assoc Offic Anal Chem 1988; 71(1): 660-4.
  • 21. Moller H, Grelier S, Pardon P, Coma V. Antimicrobial and physicochemical properties of chitosan HPMC based films. J Agric Food Chem 2004; 52(1): 6585-91.
  • 22. No HK, Kim SH, Lee SH, Park NY, Prinyawiwatkul W. Stability and antibacterial activity of chitosan solutions affected by storage temperature and time. Carbohydr Polym 2006; 65(1): 174-8.
  • 23. No HK, Park NY, Lee SH, Meters SP. Antibacterial activity of chitosans and chitosan oligomers with different molecular weights. Int J Food Microbiol 2002; 74(1): 65-72.
  • 24. Saggo S, Board R, Roller S. Chitosan inhibits growth of spoilage microorganisms in chilled pork products. Food Microbiol 2002; 19(1): 175-82.
  • 25. Sandford P. Chitosan: commercial uses and potential applications. Skjak-Braek, G., Anthosen, T.,Standford, P. (Eds.), Chitin and Chitosan. Sources, Chemistry, Biochemistry. In Physical Properties and Applications, London and New York, Elsevier Applied Science, 1989; pp. 51–69.
  • 26. Schlech WF. Foodborne listeriosis. Clin Infect Dis 2000; 31(1): 770-5.
  • 27. Shahidi F, Arachchi JKV, Jeon YJ. Food applications of chitin and chitosans. Trends Food Sci Tech 1999; 10(1): 37–51.
  • 28. Sudarshan NR, Hoover DG, Knorr D. Antibacterial action of chitosan. Food Biotechnol 1992; 6(3): 257-72.
  • 29. Uchida Y, Izume M, Ohtakara A, Preparation of chitosan oligomers with purified chitosanase and its application. in G. Skjak- Braek, T. Anthonsen, P. Sandford, Chitin and chitosan pp.373–382. 1989. London, Elsevier.
  • 30. Uyttendaele M, Busschaert P, Valero A, Geeraerd AH, Vermeulen A, Jacxsens L, Goh KK, De Loy A, Vanimpe JF, Devlieghere F. Prevalence and challenge tests of Listeria monocytogenes in Belgian produced and retailed mayonnaise-based deli-salads, cooked meat products and smoked fish between 2005 and 2007. Int J Food Microbiol 2009; 133(1): 94–104.
  • 31. Vartiainen J, Motion R, Kulonen H, Rätto M, Skyttä E, Ahvenaınen R. Chitosan-coated paper: Effects of nisin and different acids on the antimicrobial activity. J Appl Polym Sci 2004; 94(1): 986-93.
  • 32. Vialette M, Pinon A, Chasseignaux E, Lange M. Growths kinetics comparison of clinical and seafood Listeria monocytogenes isolates in acid and osmotic environment. Int J Food Microbiol 2003; 82(1): 121-31.
  • 33. Vlaemynck G, Lafarge V, Scotter S. Improvement of the detection of Listeria monocytogenes by the application of ALOA, a diagnostic, chromogenic isolation medium. J Appl Microbiol 2000; 88(1): 430-41.

Ayrıntılar

Bölüm Makaleler
Yazarlar

Ali GUCUKOGLU Bu kişi benim
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun
Türkiye


Yeliz YILDIRIM
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Erciyes University, Kayseri
Türkiye


Göknur TERZİ GÜLEL
Department of Chemistry, Faculty of Science, Anadolu University, Eskişehir
0000-0002-0011-0440
Türkiye


Murat ERDEM
Department of Chemistry, Faculty of Science, Anadolu University, Eskişehir
Türkiye


Ufuk Tansel SIRELI Bu kişi benim
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ankara University Ankara
Türkiye

Yayımlanma Tarihi 1 Ağustos 2016
Başvuru Tarihi 6 Aralık 2016
Kabul Tarihi 2 Haziran 2015
Yayınlandığı Sayı Yıl 2016, Cilt 13, Sayı 1

Kaynak Göster

Bibtex @araştırma makalesi { ercivet273833, journal = {Erciyes Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1304-7280}, eissn = {2667-5498}, address = {}, publisher = {Erciyes Üniversitesi}, year = {2016}, volume = {13}, number = {1}, pages = {11 - 18}, title = {Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi}, key = {cite}, author = {Gucukoglu, Ali and Yıldırım, Yeliz and Terzi Gülel, Göknur and Erdem, Murat and Sırelı, Ufuk Tansel} }
APA Gucukoglu, A. , Yıldırım, Y. , Terzi Gülel, G. , Erdem, M. & Sırelı, U. T. (2016). Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi . Erciyes Üniversitesi Veteriner Fakültesi Dergisi , 13 (1) , 11-18 . Retrieved from https://dergipark.org.tr/tr/pub/ercivet/issue/25189/273833
MLA Gucukoglu, A. , Yıldırım, Y. , Terzi Gülel, G. , Erdem, M. , Sırelı, U. T. "Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi" . Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13 (2016 ): 11-18 <https://dergipark.org.tr/tr/pub/ercivet/issue/25189/273833>
Chicago Gucukoglu, A. , Yıldırım, Y. , Terzi Gülel, G. , Erdem, M. , Sırelı, U. T. "Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi". Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13 (2016 ): 11-18
RIS TY - JOUR T1 - Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi AU - Ali Gucukoglu , Yeliz Yıldırım , Göknur Terzi Gülel , Murat Erdem , Ufuk Tansel Sırelı Y1 - 2016 PY - 2016 N1 - DO - T2 - Erciyes Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 11 EP - 18 VL - 13 IS - 1 SN - 1304-7280-2667-5498 M3 - UR - Y2 - 2015 ER -
EndNote %0 Erciyes Üniversitesi Veteriner Fakültesi Dergisi Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi %A Ali Gucukoglu , Yeliz Yıldırım , Göknur Terzi Gülel , Murat Erdem , Ufuk Tansel Sırelı %T Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi %D 2016 %J Erciyes Üniversitesi Veteriner Fakültesi Dergisi %P 1304-7280-2667-5498 %V 13 %N 1 %R %U
ISNAD Gucukoglu, Ali , Yıldırım, Yeliz , Terzi Gülel, Göknur , Erdem, Murat , Sırelı, Ufuk Tansel . "Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi". Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13 / 1 (Ağustos 2016): 11-18 .
AMA Gucukoglu A. , Yıldırım Y. , Terzi Gülel G. , Erdem M. , Sırelı U. T. Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. Erciyes Üniv Vet Fak Derg. 2016; 13(1): 11-18.
Vancouver Gucukoglu A. , Yıldırım Y. , Terzi Gülel G. , Erdem M. , Sırelı U. T. Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2016; 13(1): 11-18.
IEEE A. Gucukoglu , Y. Yıldırım , G. Terzi Gülel , M. Erdem ve U. T. Sırelı , "Kitosan’ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi", Erciyes Üniversitesi Veteriner Fakültesi Dergisi, c. 13, sayı. 1, ss. 11-18, Ağu. 2016