Tatlı Su Midyelerine (Unio mancus) İmidakloprid Uygulanmasının Solungaç ATPaz Aktiviteleri Üzerine Etkisi

Yıl 2021, Cilt: 37 Sayı: 1, 91 - 98, 28.04.2021

Miraç Uçkun , Aysel Alkan Uçkun

Öz

Dünya çapında yaygın olarak kullanılan sistemik bir neonikotinoid insektisit olan imidaklopridin (IMI) tatlı su kütlelerine karışması, ekosistem sağlığı açısından endişe uyandırmaktadır. IMI'nin hedef dışı organizmalar olan tatlı su midyeleri üzerindeki ölümcül olmayan etkileri hakkında bilgi eksikliği vardır. Bu çalışmada, 96 saat boyunca dört IMI konsantrasyonuna (1.25, 12.5, 125 ve 1250 µg AI L-1) maruz kalan tatlı su midyelerinde (Unio mancus) bu böcek ilacının potansiyel toksik etkilerini belirlemek amaçlanmıştır. Bunun için midyelerin toksik kirleticilerle ilk etkileşim organı olan solungaçlarında Na+/K+-ATPaz, Mg2+-ATPaz, Ca2+-ATPaz ve toplam ATPaz aktiviteleri değerlendirilmiştir. IMI maruziyetinin, bütün ATPaz türlerini kontrole göre önemli oranda inhibisyona uğrattığı gözlenmiştir. Sonuç olarak, IMI’nin ATPaz’ların aktivitesini bozarak hücrelerde iyon homeostazını ve enerji sentezini yıkıcı bir etkiye sahip olduğu, bunun da midyelerin genel metabolizmasını etkileyebileceği söylenebilir. Sonuçlarımız, tatlı su ekosistemlerinde yaşayan midyelerin IMI'ye maruz kalma nedeniyle zarar görebilecekleri olası sonuçlar konusunda bir erken uyarı verme niteliğindedir.

Anahtar Kelimeler

ATPaz, İmidakloprid, Tatlısu midyeleri, Unio mancus

Kaynakça

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