Bendiokarb İnsektisitinin İnsan Lökositlerinde Lipid Peroksidasyonu, Antioksidan Enzimler ve DNA Hasarı Üzerindeki Etkileri

Yıl 2021, Cilt: 37 Sayı: 2, 348 - 355, 28.08.2021

Fatma Öztürk Küp İsrafil Doğanyiğit

Öz

Bu çalışma da karbamat grubundan bir insektisit olan Bendiokarb’ın artan dozlarının insan kan hücreleri üzerine in vitro koşullarda toksik etkisini komet testi, total antioksidan kapasite tayini, antioksidan enzim aktivitesi ve Malondialdehit (MDA) seviyesini değerlendirmek suretiyle belirlemeyi amaçladık. Sigara ve alkol kullanmayan, çalıştığı ortamda herhangi bir kimyasal maddeye maruz kalmayan sağlıklı 6 erkek bireyden heparinli tüplere alınan kan örneklerinden lökosit izolasyonu yapıldı ve sonra artan dozlarda Bendiokarb’a (20, 40, 80, 160 µg/mL) maruz bırakılmıştır. Komet testi, Demir İndirgeme Antioksidan Kapasitesi (FRAP), 2-2'Azinobis 3-Ethilbenzotiazolin-6-Sülfonik Asit (ABTS/TEAC) analizleri ile antioksidan enzimler olan Süperoksit dismutaz (SOD), Katalaz (CAT), Glutatyon peroksidaz (GPx) ve lipid peroksidasyon göstergesi olan MDA düzeyleri üzerine etkileri değerlendirilmiştir. Çalışma sonunda antioksidan enzim düzeylerinin doz artışına bağlı olarak anlamlı azaldığı, tüm dozlarda ise MDA düzeylerinin anlamlı olarak arttığı belirlendi (p<0.05). FRAP ve TEAC yöntemlerine göre, kan hücrelerinin antioksidan kapasitesi, kontrol grubuna göre artan Bendiokarb dozlarında anlamlı olarak azaldı (p<0.05). Comet testinde artan Bendiokarb dozlarında DNA kuyruk yüzdesi, uzunluğu ve momenti anlamlı olarak artmış ve DNA hasarı tespit edilmiştir (p <0.05).
Bu çalışmayla Bendiokarb pestisitinin doğada kontrolsüz kullanımının çevreye ve canlılara zararlı etkileri olacağı gösterilmiş olmaktadır. Ancak mevcut çalışma bu konuda yapılmış bir ön çalışma niteliği taşımaktadır ve literatüre katkı sağlayacağı gibi bundan sonraki kapsamlı çalışmalara da ışık tutacağı düşüncesindeyiz.

Anahtar Kelimeler

Bendiokarb, Genotoksisite, Comet Analizi, Pestisit, Antioksidan enzimler

Effects of Bendiocarb Insecticide on Lipid Peroxidation, Antioxidant Enzymes and DNA Damage in Human Leucocytes

Öz

In this study, we aimed to determine the toxic effect of increasing doses of Bendiocarb, an insecticide from carbamate group, on human blood cells in vitro via by Comet test, determination of total antioxidant capacity, antioxidant enzyme activity and malondialdehyde (MDA) level. Leukocyte isolation was performed from blood samples taken from 6 healthy male individuals who did not smoke and alcohol use and were not exposed to any chemicals in the working environment, and then exposed to increasing doses of Bendiocarb (20, 40, 80, 160 µg / mL) and Comet test, Iron Reduction Antioxidant Capacity (FRAP), 2-2'Azinobis 3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS/TEAC) analysis with Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and MDA were evaluated. At the end of the study, it was determined that antioxidant enzyme levels decreased significantly due to dose increase, whereas MDA levels increased significantly in all doses (p<0.05). According to FRAP and TEAC methods, antioxidant capacity of blood cells decreased significantly in increasing doses of Bendiocarb compared to control group (p<0.05). In the Comet test, DNA tail percentage, length and moment were increased significantly in increasing doses of Bendiocarb and DNA damage was detected (p<0.05). Uncontrolled use of Bendiocarb pesticide in nature has been shown to have harmful effects on the environment and organisms. However, the present study is a preliminary study on this subject and we think that it will contribute to the literature and will shed light on the following comprehensive studies.

Anahtar Kelimeler

Bendiocarb, Genotoxicity, Comet Assay, Pesticides, Antioxidant Enzymes

Kaynakça

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