Analysis of Atenolol in Rabbit Plasma by HPLC Method

Öz

The goal of this research is to develop a high-performance liquid chromatography method for analyzing atenolol levels in rabbit plasma and apply this method to the pharmacokinetic study. The liquid-liquid extraction technique was used to prepare blood samples from rabbits. Separation of atenolol was achieved on an Ace C18 column. The method’s calibration curve was plotted between 5 and 250 ng/mL. The accuracy results were better than 2.97% and the precision results were less than 6.30% in rabbit plasma for atenolol. The method had recovery values >95.8% for all samples in rabbit plasma. In addition, the validated method was used to study atenolol pharmacokinetics in rabbits. The maximum atenolol plasma concentration is 240.1 ± 33.41 ng/mL. The duration to attain the greatest atenolol concentration and the area under the curve from (AUC0-12 h) were 3.0 ± 0.64 h and 1184.1 ± 235.13 ng/mL h, respectively.

Anahtar Kelimeler

• Atenolol
• HPLC
• extraction
• pharmacokinetic
• rabbit

Teşekkür

The author would like to thank Abdi Ibrahim Pharmaceutical Industry for atenolol standard. The author also wishes to thank Vedat Akba (Criminal Police Laboratory, 25060, Erzurum, Turkey) for their help in GC-MS analyses.

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