Adsorption of DNA on Coated Magnetic Beads: Comparison of Physical and Chemical Adsorption

Yıl 2019, , 215 - 224, 24.03.2019

Ceren Bayraç , Abdullah Tahir Bayraç Ecem Sarıkaya Merve Varçin

https://doi.org/10.18185/erzifbed.442557

Öz

Based on their unique properties
various iron magnetic nanoparticles have proved to be excellent nanomaterials
for applications in separation and concentration process. Immobilization of
deoxyribonucleic acid (DNA) molecules on the magnetic beads are acutely
important and have potential uses in many techniques such as DNA extraction,
concentration, biosensors, microarrays and next generation sequencing. In this
study the adsorption of single stranded DNA (ssDNA) via poly-l-lysine coated
iron oxide magnetic beads was performed under varying conditions of
poly-l-lysine amount, initial DNA concentration, ionic strength, bonding type
and length of DNA. The adsorption process was examined via Langmuir and
Freundlich isotherm models. The ionic interaction between negatively charged
DNA and positively charged surface of magnetic beads showed multilayer
adsorption with Freundlich adsorption isotherm, covalent bonding between
modified DNA and surface by crosslinking provided higher adsorption efficiency
with Langmuir adsorption isotherm. Both adsorption methods provided magnetic
beads with favorable adsorption of ssDNA.

Anahtar Kelimeler

ssDNA, magnetic beads, adsorption, Langmuir, Freundlich

Kaplanmış Manyetik Kürelere DNA Adsorpsiyonu: Fiziksel ve Kimyasal Adsorpsiyonun Karşılaştırılması

Öz

Kendilerine has
özellikleri nedeniyle çeşitli demir manyetik nanopartiküllerin ayırma ve yoğunlaştırma
uygulamalarında mükemmel nanomateryal oldukları kanıtlanmıştır.
Deoksiribonükleik asit (DNA) moleküllerinin manyetik kürelere immobilizasyonu
son derece önemli olmakla birlikte DNA ekstraksiyonu, yoğunlaştırılması,
biyosensörler, mikrodiziler ve yeni nesil dizileme gibi birçok teknikte
potansiyel kullanım alanına sahiptir. Bu çalışmada, poli-l-lizin miktarı,
başlangıç ​​DNA konsantrasyonu, iyonik kuvveti, bağlanma tipi ve DNA
uzunluğunun değişen koşulları altında poli-l-lizin kaplı demir oksit manyetik
küreler aracılığıyla tek iplik DNA'nın (ssDNA) adsorpsiyonu,
gerçekleştirilmiştir. Adsorpsiyon işlemi Langmuir ve Freundlich izoterm
modelleri ile incelenmiştir. Negatif yüklü DNA ile pozitif yüklü manyetik
küreler arasındaki iyonik etkileşimi Freundlich adsorpsiyon izotermiyle çok
katmanlı adsorpsiyon, modeli gösterirken, DNA ve yüzey arasındaki kovalent
bağlanma Langmuir adsorpsiyon izoterminde daha yüksek adsorpsiyon verimi
göstermiştir. Her iki adsorpsiyon yöntemi, manyetik kürelere uygun ssDNA
adsorpsiyonu sağlamıştır.

Anahtar Kelimeler

ssDNA, manyetik küreler, adsorpsiyon, Langmuir, Freundlich

Kaynakça

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