Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)

Öz

Abstract This study was carried out to determine the reason of mortality in guppy (Poecilia reticulata) kept in an aquarium fish-rearing farm in Istanbul province and confirm damage caused in tissues and antibiotic susceptibility of isolated pathogen bacteria. Externally diseased guppies (n=15) showed sloughing scales, ulcerative skin lesions on the body surface and abdominal distension; internally pale liver and accumulation of a liquid in the abdominal cavity were observed. Bacteriological inoculation from internal organs such as liver, spleen and kidney of guppies (n=15) were made onto Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA). After incubation, all isolated bacteria were identified as Aeromonas hydrophila according to their psychological, morphological, biochemical characteristic, and API 20E profile.  Also, these isolates (n=15) were determined to be sensitive to chloramphenicol and florfenicol. Histopathologically, multifocal liquefactive necrosis in the spleen, liver, kidney and heart, degeneration of tubular epithelium and necrosis in the kidney, desquamation of mucosa epithelium and necrotic gastric glands in the stomach, discharge of the white pulp in the spleen, desquamation of the lamina propria and mucosa epithelium in the intestine, and sloughed off the necrotic gill epithelium cells were observed.

Anahtar Kelimeler

• Aeromonas hydrophila,antibiotic susceptibility,API 20E,guppy,histopathology

Hasta Lepistes Balığından (Poecilia reticulata)İzole Edilen Aeromonas hydrophila’ nın Antibiyotik Duyarlılığı ve Histopatolojisi

Öz

Bu çalışma İstanbul ilinde bulunan bir akvaryum balığı üretim işletmesindeki hasta lepistes balıklarında görülen ölümlerin nedenini ortaya koymak ve izole edilen patojen bakterinin dokularda oluşturduğu hasarı ve antibiyotik duyarlılığını tespit etmek amacı ile yürütülmüştür. Eksternal olarak, ölmek üzere olan lepistes (Poecilia reticulata) balıklarında pullarda dökülme, vücut yüzeyinde ülseratif deri lezyonları ve abdominal genişleme görüldü; internal olarak karaciğerin renginde açılma, abdominal boşlukta sıvı birikimi tespit edildi. Karaciğer, böbrek ve dalak gibi iç organlardan Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA) besiyerlerine ekimler yapıldı. İnkubasyon sonrasında izole edilen tüm bakteriler fizyolojik, morfolojik ve biyokimyasal özellikleri ve API 20E profiline göre Aeromonas hydrophila olarak identifiye edildi. Bu izolatların (n=15) kloramfenikol ve florfenikol’e duyarlı olduğu tespit edildi. Histopatolojik olarak dalak, karaciğer, böbrek ve kalp dokusunda multifokal liquefaktif nekroz, tubuler böbrek epitelyumunda dejenerasyon ve nekroz, mide bezlerinde ve mukoza epitelyumunda dökülme, dalağın kırmızı pulpasında boşalma, bağırsağın mukoza epitelinde ve lamina propriasında dökülme yanı sıra nekrotik solungaç epitel hücrelerinde dökülme tespit edilmiştir.

Anahtar Kelimeler

• Aeromonas hydrophila,API 20E,antibiyotik duyarlılığı,histopatoloji

Kaynakça

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