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BİTKİLERDE GENOTOKSİSİTE ÇALIŞMALARINDA YAYGIN OLARAK KULLANILAN PARAMETRELER VE UYGULANAN TEST SİSTEMLERİ

Yıl 2015, , 223 - 247, 16.12.2015
https://doi.org/10.18185/eufbed.83628

Öz

Araştırmacılar son yıllarda çevremizde hem insanlar tarafından hem de doğal olarak meydana gelmiş birçok mutajenin varlığını net bir şekilde göstermişlerdir. Hava ve suların her ikisini de kirleten kimyasal ajanlar, gıdalar, ilaçlar, kozmetik ürünleri, pestisitler, ev yapımı ve endüstriyel kimyasallar gibi birçok kimyasal sınıfını içermektedir. Genotoksik kimyasalların salınımı ile meydana gelen bu kirlilik artışı ekosistemi ve insanların da içinde bulunduğu tüm organizmaları olumsuz yönde etkilemektedir. Bundan dolayı hava, su, ve toprak kontaminantları ve bunların organizmalara etkisini belirlemek amacıyla hızlı ve kesin metotlara ihtiyaç vardır. Genotoksinlerin etkilerini tanımlayabilmek için birçok genotoksisite test sistemi geliştirilmiştir. Var olan birçok araştırma göstermiştir ki bitki biyotestleri çevredeki genotoksik kontaminasyonun değerlendirilmesinde oldukça önemli metotlardır. Bitki testleri gen mutasyonları ve kromozom aberasyonları gibi geniş bir alanı kapsayan genetik hasarların belirlenmesini sağlamaktadır. Ayrıca diğer testlere göre oldukça hassas ve kolay uygulanabilen metotlar olan bitki biyotestleri, çevresel kirleticilerin genotoksik etkisinin belirlenmesinde sıklıkla kullanılmaktadır. Bu derleme ile bitkilerde yaygın olarak kullanılan genotoksisite ölçütü parametreler ve bu parametreleri içeren bazı çalışmalar değerlendirilmeye alınmıştır.

Kaynakça

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  • Tice, R.R., Agurell, E., Anderson, D., Burlinson, B., Hartman, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C., Sasaki, Y.F. 2000. Single Cell Gel/ Comet Assay: Guidelines for in Vitro and in Vivo Genetic Toxicolgoy Testing. Mutation Research, 35, 206- 221.
  • Türkoğlu, Ş. 2009. Genotoxic Effects of mono-,di-, and Trisodium Phosphste on Mitotic Activity, DNA Content, and Nuclear Volume in Allium cepa L. Caryologia, 62(3), 171–179.
  • Türkoğlu, Ş. 2012. Determination of Genotoxic Effects of Chlorfenvinphos and Fenbuconazole in Allium cepa Root Cells by Mitotic Activity, Chromosome Aberration, DNA Content, and Comet Assay. Pesticide Biochemistry and Physiology, 103, 224–230.
  • Ünal Y. 1998. Radyoterapi Gören Kanserli Hastalara Ait Kan Lenfositlerinde DNA Hasarının COMET Assay Tekniği ile Araştırılması, Gazi Üniversitesi, Sağlık Bilimler Entitüsü, Yüksek Lisans Tezi.
  • Vural N. 1984. Toksikoloji, Ankara Üniversitesi, Eczacılık Fakültesi Yayınları, No. 56, Ankara, 32-81p.
  • Wu, L., Yi, H., Yi, M. 2010. Assessment of Arsenic Toxicity using Allium/Vicia Root Tip Micronucleus Assays. Journal of Hazardous Materials, 176, 952–956.
  • Yıldız, M., Ciğerci, İ.H., Konuk, M., Fidan, A.F., Terzi, H. 2009. Determination of Genotoxic Effects of Copper Sulphate and Cobalt Chloride in Allium cepa Root Cells by Chromosome Aberration and Comet Assays. Chemosphere, 75, 934–938.
  • Yi, H., Meng, Z. 2003. Genotoxicity of Hydrated Sulfur Dioxide on Root Tips of Allium sativum and Vicia faba. Mutation Research, 537, 109–114.
  • Yi, H., Si, L. 2007. Vicia Root-Mirconucleus and Sister Chromatid Exchange Assays on The Genotoxicity of Selenium Compounds. Mutation Research, 630, 92–96.
  • Zijno, A., Leopardi, A., Marcon, F., Crebelli R. 1994. Sex Chromosome Loss and Non-Disjunction in Women: Analysis of Chromosomal Segregation in Binucleated Lymphocytes. Chromosoma, 104(6), 461-467.
Toplam 121 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Dilek Akyıl

Arzu Özkara

Yayımlanma Tarihi 16 Aralık 2015
Yayımlandığı Sayı Yıl 2015

Kaynak Göster

APA Akyıl, D., & Özkara, A. (2015). BİTKİLERDE GENOTOKSİSİTE ÇALIŞMALARINDA YAYGIN OLARAK KULLANILAN PARAMETRELER VE UYGULANAN TEST SİSTEMLERİ. Erzincan University Journal of Science and Technology, 8(2), 223-247. https://doi.org/10.18185/eufbed.83628