BİTKİLERDE GENOTOKSİSİTE ÇALIŞMALARINDA YAYGIN OLARAK KULLANILAN PARAMETRELER VE UYGULANAN TEST SİSTEMLERİ

Yıl 2015, , 223 - 247, 16.12.2015

Dilek Akyıl , Arzu Özkara

https://doi.org/10.18185/eufbed.83628

Öz

Araştırmacılar son yıllarda çevremizde hem insanlar tarafından hem de doğal olarak meydana gelmiş birçok mutajenin varlığını net bir şekilde göstermişlerdir. Hava ve suların her ikisini de kirleten kimyasal ajanlar, gıdalar, ilaçlar, kozmetik ürünleri, pestisitler, ev yapımı ve endüstriyel kimyasallar gibi birçok kimyasal sınıfını içermektedir. Genotoksik kimyasalların salınımı ile meydana gelen bu kirlilik artışı ekosistemi ve insanların da içinde bulunduğu tüm organizmaları olumsuz yönde etkilemektedir. Bundan dolayı hava, su, ve toprak kontaminantları ve bunların organizmalara etkisini belirlemek amacıyla hızlı ve kesin metotlara ihtiyaç vardır. Genotoksinlerin etkilerini tanımlayabilmek için birçok genotoksisite test sistemi geliştirilmiştir. Var olan birçok araştırma göstermiştir ki bitki biyotestleri çevredeki genotoksik kontaminasyonun değerlendirilmesinde oldukça önemli metotlardır. Bitki testleri gen mutasyonları ve kromozom aberasyonları gibi geniş bir alanı kapsayan genetik hasarların belirlenmesini sağlamaktadır. Ayrıca diğer testlere göre oldukça hassas ve kolay uygulanabilen metotlar olan bitki biyotestleri, çevresel kirleticilerin genotoksik etkisinin belirlenmesinde sıklıkla kullanılmaktadır. Bu derleme ile bitkilerde yaygın olarak kullanılan genotoksisite ölçütü parametreler ve bu parametreleri içeren bazı çalışmalar değerlendirilmeye alınmıştır.

Anahtar Kelimeler

Genotoksisite, mitotik indeks, mikronükleus, kromozom aberasyonu

Kaynakça

• Achary, V.M., Jena, S., Panda, K.K., Panda, B.B. 2008. Aluminium Induced Oxidative Stress and DNA Damage in Root Cells of Allium cepa L. Ecotoxicology and Environmental Safety, 70, 300– 310.
• Akyıl, D., Konuk, M. 2014. Detection of Genotoxicity and Mutagenicity Chromosome Aberration, Sister Chromatid Exchange, and Ames 10.1002/tox.21968. Chlorthiophos Using Micronucleus, Tests. Environmental Toxicology, DOI:
• Akyıl, D., Özkara, A., Erdoğmuş, S,F., Eren, Y., Konuk, M., Sağlam, E. 2014. Micronucleus Assay In Human Lymphocytes After Exposure to Alloxydim Sodium Herbicide In Vitro. Cytotechnology, DOİ: 10.1007/s10616-014-9746-8.
• Albertini, R.J., Anderson, D., Douglas, G.R., Hagmar, L., Hemmink, K., Merlo, F., Natarajan, A.T., Norppa, H., Shuker, D.E., Tice, R., Water, M.D., Aitio, A. 2000. IPCS Guideline for The Monitoring of Genotoxic Effects of Carcinogens in Humans, International Programme on Chemical Safety. Mutation Research, 463, 111– 172.
• Amer, S.M., Aly, F.A. 1992. Cytogenetic Effects of Pesticides. IV. Cytogenetic Effects of The Insecticides Gardona and Dursban. Mutation Research, 279(3), 165-170.
• Angelis, K.J., McGuffie, M., Menke, M. and Schubert, I. 2000. Adaptation to Alkylating Damage in DNA Measured by Comet assay. Environmental and Molecular Mutagenesis, 36, 146-150.
• Antonsie-wiez, D., 1990. Analysis of the Cell Cycle in the Root Meristem of Allium cepa under the Influence of Leda Krin. Folia Histochemica et Cytobiologica, 26, 79-96.
• Arıkan, E.S. 2006. Quizalofop-P-Ethyl Herbisitinin Allium cepa Kök Meristem Hücreleri Üzerine Sitogenetik Etkileri. Afyon Kocatepe Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, 67s, Afyonkarahisar.
• Babula, P., Vaverkova, V., Poborilova, Z., Ballova, L., Masarik, M., Provaznik, I. 2014. Phytotoxic Action of Naphthoquinone Juglone Demonstrated on Lettuce Seedling Roots. Plant Physiology and Biochemistry, 84, 78-86.
• Barile, F.A. 2008. Principles of Toxicology Testing, CRC Press Taylor & Francis Group, St. John's University, Queens, New York.
• Beg, T., Siddique, Y.H., Ara, G., Gupta, M., Afzal, J. 2009. Protective Action of EGCG Against Anticancer Drugs MMS and CP. International Journal of Pharmacology, 6(2), 1-7.
• Behboodi, B.S., Samadi, L. 2004. Detection of Apoptotic Bodies and Oligonucleosomal DNA Fragments in Cadmium-Treated Root Apical Cells of Allium cepa Linnaeus. Plant Science, 167, 411– 416.
• Beraud, E., Cotelle, S., Leroy, P., Ferard, J.F. 2007. Genotoxic Effects and Induction of Phytochelatins in The Presence of Cadmium in Vicia faba Roots. Mutation Research, 633, 112–116.
• Büssing, A., Azhari, T., Ostendorp, H., Lehnert, A., Schweizer, K. 1994. Viscum album L. Extracts Reduce Sister Chromatid Exchanges in Cultured Peripheral Blood Mononuclear Cells. European Journal of Cancer, 30, 1836-1841.
• Caritá, R., Marin-Morales, M.A., 2008. Induction of Chromosome Aberrations in the Allium cepa Test System Caused by the Exposure of Seeds to Industrial Effluents Contaminated with Azo Dyes. Chemosphere, 72, 722–725.
• Chandra, S., Chauhan, L.K.S., Murthy, R.C., Saxena, P.N., Pande, P.N., Gupta, S.K. 2005. Comparative Biomonitoring of Leachates from Hazardous Solid Waste of Two Industries using Allium cepa. Science of the Total Environment, 347, 46-52.
• Chauhan, L.K.S., Saxena, P.N., Gupta, S.K. 1999. Cytogenetic Effects of Cypermethrin and Fenvalerate on the Root Meristem Cells of Allium cepa. Environmental Experimental Botany, 42, 181–189.
• Chetalat, A.A., Albertini, S., Gocke, E. 1996. The Photomutagenicity of Floroquindones in Test for Gene Mutation, Chromosomal Abberation, Gene Conversion and DNA Breakage (Comet Assay). Mutagen, 5(11), 497- 504.
• Choy, W.N. 2001. Genetic Toxicology and Cancer Risk Assessment. Marcel Dekker, New York, 29-187.
• Cortès, F., Andersson, H.C. 1987. Analysis of SCEs in Vicia faba Chromosomes by a Simple Fluorescent Plus Giemsa Technique. Hereditas, 107, 7-13.
• Cortès, F., Escalza, P., Mateos, S., Diaz-Recasens, M. 1987. Factors Affecting the Production of Sces by Maleic Hydrazide in Root- Tip Chromosomes of Allium cepa. Mutation Research, 192, 125- 30.
• Cotelle, S., Masfaraud, J.F., Fe´rard, JF. 1999. Assessment of the Genotoxicity of Contaminated Soil with the Allium/Vicia- Micronucleus and the Tradescantia-Micronucleus Assays. Mutation Research, 426, 167–171.
• Dho, S., Camussoa,W., Mucciarellib, M., Fusconia, A. 2010. Arsenate Toxicity on The Apices of Pisum sativum L. Seedling Roots: Effects on Mitotic Activity, Chromatin Integrity and Microtubules. Environmental and Experimental Botany, 69, 17– 23.
• Dhyèvre, A., Sophie Foltête, A., Aran, D., Muller, S., Cotelle S. 2014. Effects of Soil pH on the Vicia-micronucleus Genotoxicity Assay. Mutation Research, 774,17–21.
• Dimitrov, B. 1987. Relationship Between Sister-Chromatid Exchanges and Heterochromatin or DNA Replication in Chromosomes of Crepis capillaris. Mutation Research,190, 271-276.
• Dong, Y., Zhang, J. 2010. Testing the Genotoxicity of Coking Wastewater using Vicia faba and Hordeum vulgare Bioassays. Ecotoxicology and Environmental Safety, 73, 944–948.
• Duquesnoy, I., Champeau, G.M., Evray, G., Ledoigt, G., Piquet- Pissaloux, A. 2010. Enzymatic Adaptations to Arsenic-İnduced Oxidative Stress in Zea mays and Genotoxic Effect of Arsenic in Root Tips of Vicia faba and Zea mays. Comptes Rendus Biologies, 333, 814–824.
• Eisha, A.A., Marcos, R., Creus, A. 2004. Genotoxicity Studies on the Antimicrobial Drug Sulfamethoxazole in Cultured Human Lymphocytes. Mutation Research, 564, 51-56.
• Elezaj, I.R., Millaku, L.B., Imeri-Millaku, R.H., Selimi, Q.I., Letaj K.R.R. 2011. Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida. Journal of Chemical Health Risks 1(1), 23-28.
• El-Ghamery, A.A., El-Nahas, A.I., Mansour, M.M. 2000. The Action of Atrazine Herbicide as an Inhibitor of Cell Division on Chromosomes and Nucleic Acids Content in Root Meristems of Allium cepa and Vicia faba. Cytologia, 55, 209-215.
• Ertürk Ş. 2001. Sevofuloranın DNA Hasarı Üzerine Etkilerinin Bening ve Değerlendirilmesi. Ankara Üniversitesi, Tıp Fakültesi, Anesteziyoloji Ve Reaminasyon Anabilim Dalı, Uzmanlık Tezi.
• Evans, H.J. 1984. Human Peripheral Blood Lymphocytes for the Analysis of Chromosome Aberrations in Mutagen Tests, In. Kilbey, B.J., Legator, M., Nichols, W., Ramel, C., (Editors), Handbook of Mutagenicity Test Procedures. Elsevier Science, Amsterdam, 405–427.
• Fairbairn, D.W., Olive, P.L., O'Neill, K.L. 1995. The Comet Assay: A Comprehensive Review. Mutation Research/Reviews in Genetic Toxicology, 339(1), 37-59.
• Fenech, M. 1993. The Cytokinesis Blocks Micronucleus Technique. A Detailed Description on The Method and its Application to Genotoxicity Studies in Human Population. Mutation Research, 285, 35-44.
• Fenech, M. 2000. The In Vitro Micronucleus Technique. Mutation Research, 455, 81–95.
• Fernandes, T.C.C., Mazzeo, D.E.C., Marin-Morales, M.A. 2007 Mechanism of Micronuclei Formation in Polyploidizated Cells of Allium cepa Exposed to Trifluralin Herbicide. Pesticide Biochemistry Physiology, 88, 252–259.
• Fidan, A.F. 2008. DNA Hasar Tespitinde Tek Hücre Jel Elektroforezi. Afyon Kocatepe Üniversitesi, Fen Bilimleri Dergisi, 8(1).
• Fiskesjö, G. 1985. The Allium Test as a Standard in Environmental Monitoring. Hereditas, 102, 99-112.
• Fusconi, A., Gallo, C., Camusso, W. 2007. Effects of Cadmium on Root Apical Meristems of Pisum Sativum L.: Cell Viability, Cell Proliferation and Microtubule Pattern as Suitable Markers For Assessment of Stress Pollution. Mutation Research, 632, 9–19.
• Gadeva, P., Dimitrov, B. 2008.Genotoxic Effects of the Pesticides Rubigan, Omite and Rovral in Root-Meristem Cells of Crepis capillaris L. Genetic Toxicology and Environmental Mutagenesis, 652, 191–197.
• González Borroto, J.I., Creus, A., Marcos, R. 2002. Genotoxic Evaluation of the Furylethylene Derivative 1-(5-Bromofor-2-Yl)- 2- Nitroethene in Cultured Human Lymphocytes. Mutation Research, 519, 179-185.
• Goujon, E., Sta, C., Trivella, A., Goupil , P., Richard, C., Ledoigt, G. 2014. Genotoxicity of Sulcotrione Pesticide and Photoproducts on Allium cepa Root Meristem. Pesticide Biochemistry and Physiology, 113, 47–54.
• Gökalp Muranlı, F.D. 2006. Kültürü Yapılan İnsan Lenfositlerinde Triasulfuron’un Genotoksik Etkileri. Doktora Tezi, Trakya Üniversitesi Fen Bilimleri Enstitüsü, Edirne.
• Grant ,W.F. 1992. Cytogenetics Studies of Agricultural Chemicals in Plants in Genetic Toxicology an Agricultural Perspective. Plenum Press, New York, 335–378.
• Grant, W.F. 1978. Chromosome Aberrations in Plants as a Monitoring System. Environmental Health Perspectives, 27, 37-43.
• Grant, W.F. 1982. Chromosome Aberration Assays in Allium. A report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research, 99, 273-291.
• Grant, W.F., Salamone, M.F. 1994. Comparative Mutagenicity of Chemicals Selected for Test in The International Program on Chemical Safety Collaborative Study in Plant Systems for the Detection of Environmental Mutagens. Mutation Research, 310, 187-209.
• Han, M., Li, G., Sang, N., Dong, Y. 2011. Investigating the Bio-toxicity of Coking Waste Water Using Zea mays L. Assay. Ecotoxicology and Environmental Safety, 74, 1050–1056.
• Havel, L., Durzan, D.J. 1996. Apoptosis in Plants. Botanica Acta, 109, 268-77.
• Helleday, T. 2003. Pathways for Mitotic Homologous Recombination in Mammalian Cells. Mutation Research, 532, 103-115.
• Juchimiuk, J., Maluszynska, J. 2003. Detection of DNA Fragmentation Caused by Chemical Mutagens Using the TUNEL Test. In. Maluszynska, J., Plewa, M., (Editors). Bioassays in Plant Cells for Improvement of Ecosystem and Human Health. Katowice, Wydawnictwo Uniwersytetu Śląskiego, 133-138 pp.
• Juchimiuk, J., Maluszynska, J. 2005. Transformed Roots of Crepis capillaris-A Sensitive System for the Evaluation of the Clastogenicity of Abiotic Agents. Mutation Research, 565, 129- 138.
• Kasamatsu, T., Kohda, K., Kawazoa, Y. 1996. Comparison of Chemically Induced DNA Breakage in Cellular and Subcellular Systems Using the Comet Assay. Mutation Research, 389, 1-6.
• Khadra, A, Pinelli, E., Lacroix, M.Z., Bousquet-Melou, A., Hamdi, H., Merlina, G., Guiresse, M., Hafidi, M. 2012. Assessment of the Genotoxicity of Quinolone and Fluoroquinolones contaminated soil with the Vicia faba Micronucleus Test. Ecotoxicology and Environmental Safety, 76, 187–192.
• Kihlman, B.A. 1975. Root Tips of Vicia faba for the Study of Induction of Chrosomal Aberrations, Mutation Research, 31, 401-412.
• Konuk, M., Liman, R., Cigerci, I.H. 2007. Determination of Genotoxic Effect of Boron on Allium cepa Root Meristematic Cells. Pakistan Journal of Botany, 39, 73–79.
• Koppen, G., Verschaeve, L. 1996. The Alkaline Comet Test on Plant Cells: A New Genotoxicity Test for DNA Strand Breaks in Vicia faba Root Cells. Mutation Research, 360, 193-200.
• Leme, D.M., Marin-Morales, M.A. 2009. Allium cepa Test in Environmental Monitoring: A Review on Its Application. Mutation Research, 682, 71–81.
• Li, G., Yun, Y., Li, H., Sang, N. 2008. Effect of Landfill Leachate on Cell Cycle, Micronucleus, and Sister Chromatid Exchange in Triticum aestivum. Journal of Hazardous Materials, 155, 10–16.
• Linnainmaa, K., Meretoja, T., Sorsa, M., Vainto, H. 1978. Cytogenetic Effects of Styrene and Styrene Oxide. Mutation Research, 58, 277–286.
• Ma, T.H., Cabrera, G.L., Cebulska-Wasilewska, A., Cabrera, G.L., Loarca, F., Vandenberg, A.L., Salamone, M.F. 1994. Tradescantia Stamen Hair Mutation Bioassay. Mutation Research, 310, 211-220.
• Ma, T.H., Cabrera, G.L., Gill, B.S., Sandhu, S.S., Vandenberg, A.L., Salamone, M.F. 1994b. Tradescantia Micronucleus Bioassay. Mutation Research, 310, 221-230.
• Ma, T.H., Xu, C., Liao, S., McConnell, H., Jeong, B.S., Won, C.D. 1996. In Situ Monitoring with the Tradescantia Bioassays on the Genotoxicity of Gaseous Emissions from a Closed Landfill Site and an Incinerator. Mutation Research, 359, 39–52.
• Majer, B.J., Grummt, T., Uhl, M., Knasmuller, S. 2005. Use of Plant Bioassays for the Detection of Genotoxins in the Aquatic Environment. Acta Hydrochim Hydrobiology, 33, 45–55.
• Majer, B.J., Tscherko, D., Paschke, A., Wennrich, R., Kundi, M., Kandeler, E., Knasmüller, S. 2002. Effects of Heavy Metal Contamination of Soils on Micronucleus Induction in Tradescantia and on Microbial Enzyme Activities: A Comparative Investigation. Mutation Research, 515, 111–124.
• Maluszynska, J. 1990. Chromosomes of Crepis capillaris (L.) Waller. In Vivo and In Vitro. Prace Naukowe Uniwersytetu Śląskiego, Katowice, 147.
• Maluszynska, J., Juchimiuk, J. 2005. Plant Genotoxicity: A Molecular Cytogenetic Approach in Plant Bioassays. Journal of Plant Genotoxicity, 56, 177-184.
• Marcano, L., Carruyo, I., Del Campo, A., Montiel, X. 2004. Cytotoxicity and Mode of Action of Maleic Hydrazide in Root Tips of Allium cepa L. Environmental Research, 94, 221-226.
• McKelvey-Martin, V.J., Green, M.H.L., Schmezer, P., Pool- Zobel, B.L., De Meo, M.P., Collins, A. 1993. The Single Cell Gel Electrophoresis Assay (Comet Assay): A European Review. Mutation Research, 288, 47-63.
• Montoro, A., Soriano, J.M., Barquinero, J.F., Almonacid, M., Montoro, A., Verdu, G., Sahuquillo, V., Villaescusa, J.I., Sebastia, N. 2012. Assessment In Vitro of Cytogenetic and Genotoxic Effects of Propolis on Human Lymphocytes. Food Chemical Toxicology, 50(2), 216-221.
• Mortelmans, K., Rupa, S.D. 2004. Current Issues in Genetic Toxicology Testing for Microbiologists. Advances in Applied Microbiology, 56, 379-401.
• Müller, W.U., Streffer, C. 1994. Micronucleus Assays. Mutation Research, 5, 1-13.
• Navarrete, M.H., Carrera, P., de Miguel, M., de la Torre, C. 1997. A Fast Comet Assay Variant for Solid Tissue Cells. The Assessment of DNA Damage in Higher Plants. Mutation Research, 389, 271-7.
• Ocak, A., Çiçek, A., Zeytinoğlu, H., Mercangöz, A. 2002. Porsuk Çayı Suyunun Bazı Tarım Bitkileri Üzerindeki Ekotoksikolojik Etkileri. Çevre Koruma Dergisi, 11, 9-13.
• Olaharski, A., Sotelo, R., Solorza-Luna, G., Gonsebatt, M.E., Guzman, P., Mohar, A., Eastmond, D.A. 2006. Tetraploidy and Chromosomal Instability are Early Events During Cervical Carcinogenesis. Carcinogenesis, 27, 3317-43.
• Ostling, O., Johanson, K.J. 1984. Microelectrophoretic Study of Radiation-Induced DNA Damages in Individual Mammalian Cells. Biochemical and Biophysical Research Communications, 123, 291-298.
• Özkara, A., Akyıl, D., Erdoğmuş, S.F., Konuk, M. 2011. Evaluation of Germination, Root Growth and Cytological Effects of Wastewater of Sugar Factory (Afyonkarahisar) using Hordeum vulgare Bioassays. Environmental Monitoring Assessment, 183, 517–524.
• Özkara, A., Akyıl, D., Eren, Y., Erdoğmuş, S.F. 2014. Potential Cytotoxic Effect of Anilofos by Using Allium cepa Assay. Cytotechnology, DOİ: 10.1007/s10616-014-9716-1 2014.
• Özkara, A., Akyıl, D., Eren, Y., Erdoğmuş, S.F., Konuk, M., Sağlam, E. 2014. Assessment of Cytotoxic and Genotoxic Potential of Pyracarblid by Allium test and Micronucleus Assay. Drug and Chemical Toxicology, DOI: 10.3109/01480545.2014.966831.
• Pakova, V., Hilscherova, K., Feldmannova, M., Blaha, L., 2006. Toxic Effects and Oxidative Stress in Higher Plants Exposed to Polycyclic Aromatic Hydrocarbons and Their N-Heterocyclic Derivatives. Environmental Toxicology and Chemistry, 25, 3238–3245.
• Panda, B.B., Sahu, U.K. 1985. Induction of Abnormal Spindle Function and Cytokinesis Inhibition in Mitotic Cells on Allium cepa by the Organophosphorus Insecticide Fensulfotion. Cytobios, 42, 147-155.
• Panda, B.B., Sahu, U.K.. 2002. Induction of Abnormal Spindle Function and Cytokinesis Inhibition in Mitotic Cells of Allium cepa by the Organophosphorus Insecticide Fensulfothion. Cytobios, 42, 147-155.
• Pandey, H., Kumar, V., Roy B.K. 2014. Assessment of Genotoxicity of Some Common Food Preservatives using Allium cepa L. as a Test Plant. Toxicology Reports, 1, 300-308.
• Pereira, B.B., Ju´nior, E.O.C., Morelli, S. 2013. In Situ Biomonitoring of the Genotoxic Effects of Vehicular Pollution in Uberlandia, Brazil,Using a Tradescantia Micronucleus Assay. Ecotoxicology and Environmental Safety, 87, 17–22.
• Pesnya, D.S., Romanovsky, A.V. 2013. Comparison of Cytotoxic and Genotoxic Effects of Plutonium-239 Alpha Particles and Mobile Phone GSM 900 Radiation in the Allium cepa test. Mutation Research, 750, 27-33.
• Poma, A., Arrizza, L., Picozzi, P., Spano, L. 2002. Monitoring Urban Air Particulate Matter (Fractions Pm2.5 And Pm10) Genotoxicity by Plant Systems and Human Cells In Vitro: A Comparative Analysis. Teratogenesis Carcinogenesis and Mutagenesis, 22, 271–284.
• Pourruta, B., Jeana, S., Silvestre, J., Pinelli, E. 2011. Lead-Induced DNA Damage in Vicia faba Root Cells: Potential Involvement of Oxidative Stress. Mutation Research, 726, 123– 128.
• Prajapati, S.K., Tripathi, B.D. 2008. Assessing the Genotoxicity of Urban Air Pollutants in Varanasi City Using Tradescantia Micronucleus International, 34, 1092–1096. Bioassay. Environment
• Preston, R.J., Dean, B.J., Galloway, S., Holden, H., McFee, A.F., Shelby, M. 1987. Mammalian in vivo Cytogenetic Assays: Analysis of Chromosome Aberrations in Bone Marrow Cells. Mutation Research, 189, 157–65.
• Raoof K.M.A., Siddiqui, M.B. 2013. Allelotoxic Effect of Parthenin on Cytomorphology of Broad Bean (Vicia faba L.). Journal of the Saudi Society of Agricultural Sciences, 12, 143–146.
• Rieger, R., Michaelis, A., Takehisa, S. 1990. On Adaptive Responses in Plant Meristems Cells in vivo: Protection Against Induction of Chromatid Aberration. In. G. Obe and A.T. Natarajan (Editors). Chromosomal Aberrations: Basic and Applied Aspects, Springer-Verlag, Berlin, 163-179 pp.
• Rodriguez, E., Azevedo, R., Fernandes, P., Santos, S. 2011. Cr (VI) Induces DNA Damage, Cell Cycle Arrest and Polyploidization: Aflowcytometric and Comet Assay Study in Pisum sativum . Chemical Research in Toxicology, 24, 1040–1047.
• Rodríguez, Y.A., Christofoletti, C.A., Pedro, J., Bueno, O.C., Malaspina, O., Ferreira, R.A.C., Fontanetti, C.S., 2015. Allium cepa and Tradescantia pallida Bioassays to Evaluate Effects of the Insecticide Imidacloprid. Chemosphere, 120, 438–442.
• Saleh, K., Zeytinoğlu, H. 2001. Micronucleus Test in the Peripheral Erytrocytes of Rana ridibunda as an Indicator of Environmental Pollution. Anadolu Üniversitesi, Journal of Science and Tecnology, 2, 1, 77-82.
• Sandhu, S.S., de Serres, F.J., Gopalan, H.N.B., Grant, W.F., Velemisky, J., Becking, G.C. 1994. An Introduction and Study Design. Mutation Research, 310,169-73.
• Sang, N., Li, G. 2004. Genotoxicity of Municipal Landfill Leachate on Root Tips of Vicia faba. Mutation Research, 560, 159–165.
• Savage, J.R.K. 1993. Update on Target Theory as Applied to Chromosomal Mutagenesis, 22, 198-207. Environmental Molecular
• Saxena, P.N., Chauhan, L.K.S., Gupta, S.K. 2005. Cytogenetic Effects of Commercial Formulation of Cypermethrin in Root Meristem Cells of Allium sativum: Spectroscopic Basis of Chromosome Damage. Toxicology, 216, 244-252.
• Saxena, P.N., Gupta, S.K., Murthy, R.C. 2010. Carbofuran Induced Cytogenetic Effects in Root Meristem Cells of Allium cepa And Allium sativum: A Spectroscopic Approach for Chromosome Damage. Pesticide Biochemistry and Physiology, 96, 93–100.
• Scaldaferro, M.A., Prina, A.R., Moscone, E.A., Kwasniewska, J.. 2013. Effects of Ionizing Radiation on Capsicum baccatum var. pendulum (Solanaceae). Applied Radiation and Isotopes, 79, 103–108.
• Seth, C.S., Chaturvedi, P.K., Misra, V. 2007. Toxic Effect of Arsenate and Cadmium Alone and in Combination on Giant Duckweed (Spirodela polyrrhiza L.) in Response to Its Accumulation. Environmental Toxicology, 22, 539–549.
• Seth, C.S., Misraa, V., Chauhanb, L.K.S., Singh, R.R. 2008. Genotoxicity of Cadmium on Root Meristem Cells of Allium cepa: Cytogenetic and Comet Assay Approach. Ecotoxicology and Environmental Safety, 71, 711–716.
• Singh, N.P., Danner, D.E. Tice, R.R., Brant, L., Schneider, E.L. 1988. Single Cell Gel Electrophoresis Assay (Comet Assay): Its Importance in Human Biology. Mutation Research, 37, 123.
• Singh, S., Lehmann-Grube, B., Goedde, H.W. 1984. Cytogenetic Effects of Paraoxon and Methyl-Parathion on Cultured Human Lymphocytes: SCE, Clastogenic Activity and Cell Cycle Delay. International Archives of Occupational and Environmental Health, 54, 195-200.
• Sinyth, D.R., Evans, H.J. 1976. Mapping of Sister Chromatid Exchanges in Human Chromosomes Using G Banding and Autoradiography. Mutation Research, 35, 139-154.
• Smaka-Kincl, V., Stegnar, P., Lovka, M., Toman, M.J. 1996. The Evaluation of Waste, Surface and Ground Water Quality using the Allium Test Procedure. Mutation Research, 368, 171-179.
• Soyöz, M., Özçelik, N. 2003. Zirai Mücadelede Kullanılan Pestisidlerin Üniversitesi, Tıp Fakültesi Dergisi, 10(1), 6-9. Etkileri. Süleyman Demirel
• Srivastava, K., Mishra, K.K. 2009. Cytogenetic Effects of Commercially Formulated Atrazine on The Somatic Cells of Allium cepa and Vicia faba. Pesticide Biochemistry and Physiology, 93, 8–12.
• Stoilov, L., Wojcik, A,K., Giri, A., Obe, G. 2002. SCE Formation After Exposure of CHO Cells Prelabeled with BrdU or Biotin-Dutp to Various DNA-Damaging Agents. Mutagenesis, 17(5), 399-403.
• Taylor, J.H. 1958. Sister-Chromatid Exchanges in Tritium-labeled Chromosomes. Genetics, 43, 515–529.
• Taylor, J.H., Woods, P.S., Hughes, W.L. 1957. The Organization and Chromosomes Duplication Autoradiographic Studies Using Tritium-Labeled Thymidinee. Proceedings of the National Academy of Sciences, 43, 122–128.
• Tice, R.R., Agurell, E., Anderson, D., Burlinson, B., Hartman, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C., Sasaki, Y.F. 2000. Single Cell Gel/ Comet Assay: Guidelines for in Vitro and in Vivo Genetic Toxicolgoy Testing. Mutation Research, 35, 206- 221.
• Türkoğlu, Ş. 2009. Genotoxic Effects of mono-,di-, and Trisodium Phosphste on Mitotic Activity, DNA Content, and Nuclear Volume in Allium cepa L. Caryologia, 62(3), 171–179.
• Türkoğlu, Ş. 2012. Determination of Genotoxic Effects of Chlorfenvinphos and Fenbuconazole in Allium cepa Root Cells by Mitotic Activity, Chromosome Aberration, DNA Content, and Comet Assay. Pesticide Biochemistry and Physiology, 103, 224–230.
• Ünal Y. 1998. Radyoterapi Gören Kanserli Hastalara Ait Kan Lenfositlerinde DNA Hasarının COMET Assay Tekniği ile Araştırılması, Gazi Üniversitesi, Sağlık Bilimler Entitüsü, Yüksek Lisans Tezi.
• Vural N. 1984. Toksikoloji, Ankara Üniversitesi, Eczacılık Fakültesi Yayınları, No. 56, Ankara, 32-81p.
• Wu, L., Yi, H., Yi, M. 2010. Assessment of Arsenic Toxicity using Allium/Vicia Root Tip Micronucleus Assays. Journal of Hazardous Materials, 176, 952–956.
• Yıldız, M., Ciğerci, İ.H., Konuk, M., Fidan, A.F., Terzi, H. 2009. Determination of Genotoxic Effects of Copper Sulphate and Cobalt Chloride in Allium cepa Root Cells by Chromosome Aberration and Comet Assays. Chemosphere, 75, 934–938.
• Yi, H., Meng, Z. 2003. Genotoxicity of Hydrated Sulfur Dioxide on Root Tips of Allium sativum and Vicia faba. Mutation Research, 537, 109–114.
• Yi, H., Si, L. 2007. Vicia Root-Mirconucleus and Sister Chromatid Exchange Assays on The Genotoxicity of Selenium Compounds. Mutation Research, 630, 92–96.
• Zijno, A., Leopardi, A., Marcon, F., Crebelli R. 1994. Sex Chromosome Loss and Non-Disjunction in Women: Analysis of Chromosomal Segregation in Binucleated Lymphocytes. Chromosoma, 104(6), 461-467.