Yaygın İnorganik Anyonların Çay Örneklerinde İyon Kromatografik Tayini

Yıl 2021, , 168 - 174, 31.03.2021

Mustafa Cittan

https://doi.org/10.18185/erzifbed.880524

Öz

Bu çalışmada, poşet çaylarda bulunan inorganik anyonları - florür, klorür, nitrit, bromür, nitrat, fosfat ve sülfat - belirlemek için, iletkenlik detektörü ile eşleştirilmiş iyon kromatografisine dayalı basit, hızlı ve doğru bir teknik tanımlanmıştır. Analitlerin kromatografik ayrılması, Dionex IonPac AG9-HC 4×50 mm ön kolonu ile birleştirilmiş, Dionex IonPac AS9-HC 4x250 mm analitik kolon kullanılarak gerçekleştirilmiştir. Elüent olarak 12 mM KOH çözeltisi kullanılmıştır. İnorganik anyonlar için tayin limitleri, 0.02 - 0.20 mg L-1 aralığında belirlenmiştir. Çay infüzyonları geleneksel olarak poşet çayların 200 mL hacimli kaynar ultra saf su ile demlenmesi yoluyla hazırlanmıştır. Söz konusu anyonların ekstraksiyonuna demleme süresinin etkisi incelenmiş ve elde edilen sonuçlara göre optimum demleme süresi 6 dakika olarak belirlenmiştir. Son olarak tarif edilen kromatografik yöntem bazı siyah, yeşil ve beyaz poşet çay örneklerindeki inorganik anyonların analizleri için uygulanmış ve beş adet anyon numunelerde nicel olarak belirlenmiştir. Tüm örneklerdeki nitrit ve bromür miktarları ise yöntemin tayin limitlerinin oldukça altında tespit edilmiştir.

Anahtar Kelimeler

İyon kromatografi, inorganik anyonlar, siyah çay, yeşil çay, beyaz çay

Ion Chromatographic Determination of Common Inorganic Anions in Tea Samples

Öz

A simple, rapid, and accurate technique based on ion chromatography coupled with a suppressed conductivity detector was described to determine the inorganic anions – fluoride, chloride, nitrite, bromide, nitrate, phosphate, and sulphate in tea bag samples. A Dionex IonPac AS9-HC 4×250 mm analytical column with a Dionex IonPac AG9-HC 4×50 mm guard column was used for the chromatographic separation of the analytes. The eluent was 12 mM KOH and the limit of quantification for the inorganic anions were in the range 0.02 - 0.20 mg L-1. Tea infusions were prepared traditionally by pouring the tea bags with 200 mL of boiling ultra-pure water. Influence of the brewing time on the extraction of inorganic anions from the tea bags was examined and optimum brewing time was determined as 6 min according to the results obtained. Finally, the ion chromatographic technique was applied to analyze the inorganic anions in some black, green, and white tea bag samples and five anions were determined quantitatively. The nitrite and bromide amount in all the samples were well below the quantification limit of the method.

Anahtar Kelimeler

Ion chromatography, inorganic anions, black tea, green tea, white tea

Kaynakça

• Alcázar, A., Fernández-Cáceres, P. L., Martín, M. J., Pablos, F., and González, A. G. 2003. "Ion chromatographic determination of some organic acids, chloride and phosphate in coffee and tea", Talanta, 61(2), 95–101.
• Balcerzak, M., and Janiszewska, J. 2015. "Determination of common inorganic anions in tea samples by ion chromatography", Acta Alimentaria, 44(3), 365–373.
• Ding, M. Y., Chen, P. R., and Luo, G. A. 1997. "Simultaneous determination of organic acids and inorganic anions in tea by ion chromatography", Journal of Chromatography A, 764(2), 341–345.
• Jagadish, R., and Shanmugaselvan, V. A. 2018. "Quantification of Inorganic Anions in Tea ( Camellia Sinensis (L) O. Kuntze) Tissues and Soil Using Ion Chromatography Coupled with Conductivity Detector", Communications in Soil Science and Plant Analysis, 49(8), 875–888.
• Kumar, S. D., Narayan, G., and Hassarajani, S. 2008. "Determination of anionic minerals in black and kombucha tea using ion chromatography", Food Chemistry, 111(3), 784–788.
• Ma, L., Shi, Y., Ruan, J., and Han, W. 2002. "Status of Fluorine in the Soils of Tea Gardens in Brick Tea Areas of Hunan, Hubei Provinces and Its Affecting Factors", Journal Tea Science, 22, 34–38.
• Michalski, R. 2006. "Simultaneous determination of common inorganic anions in black and herbal tea by suppressed ion chromatography", Journal of Food Quality, 29(6), 607–616.
• Milovanovic, Z., Popovic, S., Pantelic, A., Milinkov, J., Milosevic, D., Petrovic, V., and Vidovic, M. 2018. "Determination of inorganic anions in herbal tea infusions using ion chromatography", Matica Srpska Journal for Natural Sciences, 134, 89–99.
• Minca, I., Bajenaru, I., Guran, C., Maria Josceanu, A., and Daniela Isopescu, R. 2015. "Validation of an Ion Chromatographic Method for Determination of Anions in Tea Infusions", Revista de Chimie (Bucharest), 66(6), 759–765.
• Mincă, I., Josceanu, A. M., Isopescu, R. D., and Guran, C. 2013. "Determination of ionic species in tea infusions by ion chromatography", UPB Scientific Bulletin, Series B: Chemistry and Materials Science, 75(3), 65–78.
• Mondal, T. K., Bhattacharya, A., Laxmikumaran, M., and Ahuja, P. S. 2004. "Recent advances of tea (Camellia sinensis) biotechnology", Plant Cell, Tissue and Organ Culture, 76, 195-254.
• Q2B Validation of Analytical Procedures: Methodology | FDA. (1997).
• Spiro, M., and Lam, P. L. L. 1995. "Kinetics and equilibria of tea infusion-Part 12. Equilibrium and kinetic study of mineral ion extraction from black Assam Bukial and green Chun Mee teas", Food Chemistry, 54(4), 393–396.