Effect of Tianeptine on Oxidative Renal Damage Induced by Ischemia Reperfusion in Rats

Yıl 2018, Cilt: 11 Sayı: 2, 300 - 305, 31.08.2018

Bahadir Süleyman , Aslı Özbek Bilgin , Renad Mammadov

https://doi.org/10.18185/erzifbed.422543

Öz

Renal ischemia-reperfusion (I/R) damage can develop following
major surgical conditions such as shock, trauma, and renal transplantation and
causes acute renal failure. Kidney I/R damage is known to be a pathological
process that begins with the touch free of oxygen and continues with the
production of free oxygen radicals. We will investigate the effect of renal I/R
injury on tianeptine, an atypical antidepressant drug with antioxidant
properties. There was no information on the effect of tianeptine on I/R-induced
kidney damage in the literature. The aim of the
present study was to investigate the effect of tianeptine on oxidative kidney
damage induced by I/R in rats. Albino wistar male rats were divided into groups
as renal I/R (RIR) group, 25 mg/kg of thianeptine+renal I/R (TIR-25) group and
sham operation (SG) group. The I/R procedure (one hour ischemia, three hours
reperfusion) was performed with 25 mg/kg intraperitoneal thiopental sodium
anesthesia. Oxidative damage was assessed by malondialdehyde (MDA) and total
glutathione (TGSH) measurements in kidney tissue. Biochemical test results
showed that the I/R process increased the amount of MDA in the kidney tissue
and decreased the tGSH. Tianeptine significantly prevented the increase of MDA
and decrease of tGSH in kidney tissue after I/R. Our experimental results suggest
that tianeptine may be useful in preventing I/R-induced oxidative renal injury.

Anahtar Kelimeler

Tianeptine, Ischemia/Reperfusion, Kidney, Malondialdehyde, Total glutathione

Tianeptinin Sıçanlarda İskemi Reperfüzyonla Oluşturulan Oksidatif Böbrek Hasarına Etkisi

Öz

Böbrek iskemi-reperfüzyon (İ/R) hasarı  şok, travma
ve böbrek transplantasyonu gibi majör cerrahi durumları takiben
gelişebilmektedir ve akut böbrek yetmezliğine neden
olmaktadır. Böbrek İ/R hasarının,   dokunun
oksijensiz  kalması ile başlayan ve  serbest oksijen
radikallerinin üretimi ile devam eden patolojik bir süreç olduğu
bilinmektedir. Çalışmamızda böbrek İ/R hasarına etkisini araştıracağımız
tianeptin,  antioksidan özelliğe sahip atipik antidepresan bir
ilaçtır. Literetürlerde tianeptinin, İ/R ile oluşturulan böbrek hasarına
karşı etkisine ait bilgilere rastlanmadı. Çalışmamızın amacı tianeptinin, sıçanlarda İ/R ile oluşturulan
oksidatif böbrek hasarına etkisini araştırmaktır. Albino wistar türü erkek
sıçanlar renal iskemi-reperfüzyon (RİR), 25 mg/kg tianeptin+renal
iskemi-reperfüzyon (TİR-25) ve şam operasyonu uygulanacak (SG)
gruplara  ayrıldı. İ/R işlemi (bir saat iskemi, üç saat reperfüzyon),
25 mg/kg intraperitoneal tiopental sodyum anestezisi uygulanarak yapıldı.
Oksidatif hasar böbrek dokusunda malondialdehid (MDA) ve total glutatyon (tGSH)
ölçümü ile değerlendirildi. Biyokimyasal deney sonuçları, İ/R işleminin böbrek
dokusunda MDA miktarının artmasına tGSH’nın ise azalmasına neden olduğunu
göstermiştir. Tianeptinin, böbrek dokusunda MDA’nın İ/R ile artmasını ve
tGSH’nın azalmasını anlamlı önlediği saptanmıştır. Deney
sonuçlarımız, tianeptinin İ/R ile indüklenen oksidatif böbrek hasarını
önlemede yararlı olabileceğini düşündürmektedir.

Anahtar Kelimeler

Malondialdehid, Tianeptin, İskemi/Reperfüzyon, Böbrek, Total glutatyon

Kaynakça

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