CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH

Ömer Yamaner; Bengi Erdağ

doi:10.18036/estubtdc.569370

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CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH

Abstract

In this study, A standart protocol to callus formation and adventitious shoot regeneration of Hypericum adenotrichum Spach via direct or indirect organogenesis has been described.

Callus induction was carried out by using leaves which were collected from their native environment. The maximum callus induction frequency has been observed on MS media containing 4 mgL^-1 BA and 0.2 mgL^-1 NAA. These calli were not induced to shoot regeneration. Shoot formation was obtained by transferring the calluses from BA-containing media to MS medium containing 0.5 mgL^-1 KIN. Maximum shoot number was observedin transferred calluses from callus induction medium containing 3 mgL^-1 BA to media containing 0.5 mgL^-1 KIN.

Direct shoot formation developed on leaf explants of H. adenotrichum on MS media containing KIN alone. The highest direct shoot development was observed on MS medium with 1 mgL^-1KIN.

Keywords

Hypericum adenotrichum,callus,, endemic plant,, adventitious shoot

Supporting Institution

Aydın Adnan Menderes Üniversitesi

Project Number

FBE- 09013

Thanks

This study was part of the pH D thesis of Ömer Yamaner and financially supported by Aydın Adnan Menderes University Scientific Research Project Department (Project no: FBE- 09013). Abstract part of the study was presented as poster presentation at the 21st National Biology Congress, 3-7 September 2012, İzmir TURKEY.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ömer Yamaner
Türkiye

Bengi Erdağ ^*
0000-0001-7042-9011
Türkiye

Publication Date

January 31, 2020

Submission Date

May 23, 2019

Acceptance Date

January 30, 2020

Published in Issue

Year 2020 Volume: 9 Number: 1

DOI

https://doi.org/10.18036/estubtdc.569370

IZ

https://izlik.org/JA98GF84YC

APA

Yamaner, Ö., & Erdağ, B. (2020). CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 9(1), 98-108. https://doi.org/10.18036/estubtdc.569370

AMA

1.Yamaner Ö, Erdağ B. CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2020;9(1):98-108. doi:10.18036/estubtdc.569370

Chicago

Yamaner, Ömer, and Bengi Erdağ. 2020. “CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum Adenotrichum SPACH”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 9 (1): 98-108. https://doi.org/10.18036/estubtdc.569370.

EndNote

Yamaner Ö, Erdağ B (January 1, 2020) CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 9 1 98–108.

IEEE

[1]Ö. Yamaner and B. Erdağ, “CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 9, no. 1, pp. 98–108, Jan. 2020, doi: 10.18036/estubtdc.569370.

ISNAD

Yamaner, Ömer - Erdağ, Bengi. “CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum Adenotrichum SPACH”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 9/1 (January 1, 2020): 98-108. https://doi.org/10.18036/estubtdc.569370.

JAMA

1.Yamaner Ö, Erdağ B. CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2020;9:98–108.

MLA

Yamaner, Ömer, and Bengi Erdağ. “CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum Adenotrichum SPACH”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 9, no. 1, Jan. 2020, pp. 98-108, doi:10.18036/estubtdc.569370.

Vancouver

1.Ömer Yamaner, Bengi Erdağ. CALLUS INDUCTION AND ADVENTITIOUS SHOOT REGENERATION OF Hypericum adenotrichum SPACH. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2020 Jan. 1;9(1):98-108. doi:10.18036/estubtdc.569370