Endoparazitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) venomunun glioblastoma hücreleri üzerindeki sitotoksisitesi

Öz

Beyin kanseri tedavisinde, antikanser ajanlar kullanılarak kanser hücrelerinin proliferasyonunun inhibe edilmesi öncelikli bir yaklaşımdır. Endoparazitoid arıların venomu da, yeni terapötik ajanların geliştirilmesine aday olabilir. Böylece bir endoparasitoid tür olan Pimpla turionellae L. (Hymenoptera: Ichneumonidae)’nın venomu, çeşitli mekanizmalarla geleneksel tedavilere direnç gösteren glioblastoma üzerinde antikanserojen bir etkiye sahip olabilir. Bu nedenle, çalışmamızda endoparazitoid venomunun glioblastoma hücreleri üzerindeki sitotoksisitesinin araştırılması amaçlanmıştır. Bu amaçla, P. turionellae venomunun sitotoksik potansiyeli, metil tiyazolil tetrazolyum testi (MTT assay) ile sıçan C6 glioblastoma hücre hattı üzerinde değerlendirildi. P. turionellae venomu 15 ± 2 günlük dişi bireylerden izole edildi. farklı konsantrasyonlardaki P. turionellae venomu (176.6-1.83 μg/ml) in vitro olarak C6 sıçan glioblastoma hücrelerine uygulandı. MTT testinden elde edilen bulgulara göre, uygulanan parazitoid venom konsantrasyonuna bağlı olarak C6 hücrelerinin in vitro canlılığının önemli ölçüde azalmıştır. Bu nedenle, P. turionellae zehiri zamana ve doza bağlı bir şekilde hücreler üzerinde sitotoksik aktivite göstermiştir. Sonuç olarak, endoparazitoid arı venomunun sitotoksisitesine ait elde edilen bu bulgular, malignant tümörlere karşı yeni kemoterapötik ajanların araştırılmasında primer veri olarak kullanılabir.

Anahtar Kelimeler

• Pimpla turionellae
• endoparazitoid venom
• glioblastoma
• sitotoksisite
• MTT
• MTT

CYTOTOXICITY OF VENOM FROM ENDOPARASITOID PIMPLA TURIONELLAE L. (HYMENOPTERA: ICHNEUMONIDAE) ON GLIOBLASTOMA CELLS

Öz

In the treatment of brain cancer, the inhibition of cancer cell proliferation using anti-cancer agents is a priority approach. Venom of endoparasitoid wasps also may be a candidate for the development of new therapeutic agents. In this context, endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) venom may have an anti-carcinogenic effect on glioblastoma that resists traditional therapies by various mechanisms. Therefore, we aimed to investigate endoparasitoid venom cytotoxicity on glioblastoma cells. For this purpose, the cytotoxic potential of venom from P. turionellae was evaluated on rat C6 glioblastoma cell lines with methyl thiazolyl tetrazolium assay (MTT assay). P. turionellae venom was isolated from 15±2 day-old females. Different concentrations of P. turionellae venom (176.6-1.83 μg/ml) were applied to C6 rat glioblastoma cells in vitro. Results of MTT assay showed that the viability of C6 cells in vitro significantly decreased depending on the parasitoid venom concentrations. Therefore, P. turionellae venom showed cytotoxic activity in a time- and dose-dependent manner. In conclusion, the results from this research could be used as primary data of venom cytotoxicity for investigation of new chemotherapeutic agents against malignant tumors.

Anahtar Kelimeler

• Pimpla turionellae
• endoparasitoid venom
• glioblastoma
• cytotoxicity
• MTT

Kaynakça

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