CYTOTOXICITY OF VENOM FROM ENDOPARASITOID PIMPLA TURIONELLAE L. (HYMENOPTERA: ICHNEUMONIDAE) ON GLIOBLASTOMA CELLS

Yıl 2020, Cilt: 9 Sayı: 2, 219 - 225, 30.07.2020

Selin Çim Hülya Altuntaş , Ayşe Ak

https://doi.org/10.18036/estubtdc.616173

Öz

In the treatment of brain
cancer, the inhibition of cancer cell proliferation using anti-cancer agents is
a priority approach. Venom of endoparasitoid wasps also may be a candidate for
the development of new therapeutic agents. In this context, endoparasitoid Pimpla turionellae L. (Hymenoptera:
Ichneumonidae) venom may have an anti-carcinogenic effect on glioblastoma that
resists traditional therapies by various mechanisms. Therefore, we aimed to
investigate endoparasitoid venom cytotoxicity on glioblastoma cells. For this
purpose, the cytotoxic potential of venom from P. turionellae was evaluated on rat C6 glioblastoma cell lines with
methyl thiazolyl tetrazolium assay (MTT assay). P. turionellae venom was isolated from 15±2 day-old females.
Different concentrations of P.
turionellae venom (176.6-1.83 μg/ml) were applied to C6 rat glioblastoma
cells in vitro. Results of MTT assay showed that the viability of C6 cells in
vitro significantly decreased depending on the parasitoid venom concentrations.
Therefore, P. turionellae venom
showed cytotoxic activity in a time- and dose-dependent manner. In conclusion,
the results from this research could be used as primary data of venom
cytotoxicity for investigation of new chemotherapeutic agents against malignant
tumors.

Anahtar Kelimeler

Pimpla turionellae, endoparasitoid venom, glioblastoma, cytotoxicity, MTT

Destekleyen Kurum

Eskisehir Technical University, Scientific Research Projects Commission

Proje Numarası

1707F458

Teşekkür

We would like to thank Bora Garipcan for his technical supports in the Institute of Biomedical Engineering at Boğaziçi University, Istanbul, Turkey

Endoparazitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) venomunun glioblastoma hücreleri üzerindeki sitotoksisitesi

Öz

Beyin kanseri tedavisinde, antikanser
ajanlar kullanılarak kanser hücrelerinin proliferasyonunun inhibe edilmesi
öncelikli bir yaklaşımdır. Endoparazitoid arıların venomu da, yeni terapötik
ajanların geliştirilmesine aday olabilir. Böylece bir endoparasitoid tür olan Pimpla turionellae L. (Hymenoptera:
Ichneumonidae)’nın venomu, çeşitli mekanizmalarla geleneksel tedavilere direnç
gösteren glioblastoma üzerinde antikanserojen bir etkiye sahip olabilir. Bu
nedenle, çalışmamızda endoparazitoid venomunun glioblastoma hücreleri
üzerindeki sitotoksisitesinin araştırılması amaçlanmıştır. Bu amaçla, P. turionellae venomunun sitotoksik
potansiyeli, metil tiyazolil tetrazolyum testi (MTT assay) ile sıçan C6
glioblastoma hücre hattı üzerinde değerlendirildi. P. turionellae venomu 15 ± 2 günlük dişi bireylerden izole edildi.
farklı konsantrasyonlardaki P.
turionellae venomu (176.6-1.83 μg/ml) in vitro olarak C6 sıçan glioblastoma
hücrelerine uygulandı. MTT testinden elde edilen bulgulara göre, uygulanan parazitoid
venom konsantrasyonuna bağlı olarak C6 hücrelerinin in vitro canlılığının önemli
ölçüde azalmıştır. Bu nedenle, P.
turionellae zehiri zamana ve doza bağlı bir şekilde hücreler üzerinde sitotoksik
aktivite göstermiştir. Sonuç olarak, endoparazitoid arı venomunun
sitotoksisitesine ait elde edilen bu bulgular, malignant tümörlere karşı yeni
kemoterapötik ajanların araştırılmasında primer veri olarak kullanılabir.

Anahtar Kelimeler

Pimpla turionellae, endoparazitoid venom, glioblastoma, sitotoksisite, MTT, MTT

Proje Numarası

1707F458

Kaynakça

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