Farklı Seviyelerde Karanfil Esansiyel Yağının Kuru Çayır Otuna İlavesinin In Vitro Gaz ve Metan Üretimi ile Yem Değeri Üzerine Etkileri

Yıl 2025, Cilt: 8 Sayı: 2, 51 - 58

Yeliz Burcu , Hatice Kaya

https://doi.org/10.55257/ethabd.1739288

Öz

Bu çalışma, kuru çayır otuna farklı seviyelerde karanfil yağı (Syzgium aromaticum) ilavesinin in vitro gaz (İG) ve metan (CH4) üretimi ile yem değeri, sindirilebilirlik ve bazı rumen parametreleri üzerine etkisinin in vitro gaz üretim tekniği kullanılarak belirlenmesi amacıyla yürütülmüştür. Kuru çayır otuna 0 (K0), 100 (K1), 200 (K2), 400 (K3) ppm düzeylerinde ticari bir firmadan temin edilen karanfil yağı ilavesinin 24 saatlik İG, CH4 üretimi, tahminlenen performans parametre (metabolik enerji (ME), net enerji laktasyon (NEL), organik madde sindirilebilirliği (OMS) değerleri ve sindirilebilirlik Gerçek sindirilen kuru madde (GSKM) ve gerçek sindirim değeri (GSD) parametrelerinin muameleden etkilendiği belirlenmiştir (p<0.01). K0, K1 ve K2 gruplarının İGÜ ve OMS değerleri bakımından benzerlik gösterdiği, ancak bu gruplarda elde edilen değerlerin K3 grubuna kıyasla yüksek olduğu görülmüştür (P<0.05). Polinomiyal analiz sonucunda metan üretimi linear olarak etkilenirken diğer parametrelerde (ME, NEL, OMS) quadratik etki görülmüştür. En yüksek gaz ve metan üretimi K1 grubunda (30.44 ve 5.19 ml), en düşük gaz ve metan üretimi K3 grubunda (24.36 ve 3.86 ml) saptanmıştır (P<0.05). Deneme grupları arasında gerçek sindirilebilir kuru madde ve gerçek sindirim değerlerine ait ortalamaların polinomiyal analiz sonucuna göre linear olarak azaldığı görülmüştür (P<0.05). Kuru çayır otuna farklı seviyelerde karanfil yağı ilavesinin diğer parametreleri (taksimat faktörü, mikrobiyal protein üretimi, mikrobiyal partikül sentezleme etkinliği, rumen pH’sı, toplam uçucu yağ asidi ve amonyak azotu) etkilemediği tespit edilmiştir. Sonuç olarak İG ve CH4 üretimi ile ME, NEL, OMS ve sindirim parametreleri birlikte değerlendirildiğinde kuru çayır otuna 100 ppm seviyesinde karanfil yağı ilavesinin daha tatminkâr sonuç verdiği görülmüştür. Ancak in vivo çalışmalarla bu sonucun desteklenmesi gerektiği kanaatine ulaşılmıştır.

Anahtar Kelimeler

Karanfil esansiyel yağı , kuru çayır otu , in vitro gaz , metan , yem değeri

Effects of the Addition of Different Levels of Clove Essential Oil to Dry Meadow Grass on In Vitro Gas and Methane Production and Feed Value

Öz

This study was carried out to determine the effects of clove oil (Syzgium aromaticum) supplementation at different levels to dry meadow grass on in vitro gas (IG) and methane (CH4) production, feed value, digestibility and some rumen parameters using in vitro gas production technique. Significant differences were detected in the effects of addition of clove oil supplied by a commercial company to dry meadow grass at levels of 0 (K0), 100 (K1), 200 (K2), 400 (K3) ppm on 24-hour IG and CH4 production and estimated performance parameters (metabolic energy (ME), net energy lactation (NEL), organic matter digestibility (OMD)) and digestibilities parameters (true digestible dry matter (TDDM) and true digestibility values (TDV)) (p<0.01). It was determined that K0, K1 and K2 groups were similar in IG and OMD values, but the values obtained in these groups were higher than in the K3 group (P<0.05). In the polynomial analysis, methane production was affected linearly, while quadratic effects were observed in other parameters (ME, NEL, OMS). The highest gas and methane production was determined in K1 group (30.44 and 5.19 ml), the lowest gas and methane production was determined in the K3 group (24.36 and 3.86 ml) (P<0.05). It was observed that the means of true digestible dry matter and true digestibility values among the experimental groups decreased linearly according to the polynomial analysis result (P<0.05). It was found that adding different levels of clove oil to dry meadow grass did not affect other parameters (partitioning factor, microbial protein production, microbial particle synthesis efficiency, rumen pH, total volatile fatty acid and ammonia nitrogen). As a result, when the production of IG and CH4 and ME, NEL, OMD and digestion parameters were evaluated together, it was seen that the addition of 100 ppm clove oil to dry meadow grass gave more satisfactory results. However, it was concluded that this result should be supported by in vivo studies.

Anahtar Kelimeler

Extraction method , extract , in vitro gas , methane , tarragon

Kaynakça

• A.O.A.C. (1990). Official Methods of Analysis. 15th Edition, Association of Official Analytical Chemist, Washington DC.
• Agarwal, N., Shekhar, C., Kumar, R., Chaudhary, L.C. and Karma, D. N. (2009). Effect of Peppermint (Mentha piperita) Oil on İn Vitro Methanogenesis and Fermentation of Feed with Buffalo rumen Liquor. Animal Feed Science and Technolgy, 148: 321-327.
• Arslan, C. ve Çelebi, E. (2017). Ruminantlarda Rumende Oluşan Metan Üretimini Azaltmaya Yönelik Çalışmalar. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 12 (3):327-337 (Erzurum).
• Benchaar, C. and Greathead, H. (2011). Essential Oilsand Opportynities to Mitigate Enteric Methane Emissions From Ruminants. Animal Feed Science and Technology, 166-167: 338-355.
• Benchaar, C., Petit, H.V., Berthiaume, R., Ouellet, D.R., Chiquette, J. and Chouinard, P.Y. (2007). Effects of essential oils on digestion, ruminal fermentation rumen microbial populations, milk production, and milk composition in dairy cows fed alfalfa silage or corn silage. Journal of Dairy Science, 90: 886-897.
• Blummel, M., Makkar, HPS. ve Becker, K. (1997). İn vitro gas production- A technique revised. Journal of Animal Physiology and Animal Nutrition, 77: 24-34.
• Bokharaeian, M., Ghoorchi, T., Toghdory, A. and Esfahani, I.J. (2023). The dose-dependent role of sage, clove and pine essential oils in modulating ruminal fermantation and biohydrogenation of polyunsaturated fatty acid: A promising strategy to reduce methane emissions and enhance the nutritional profile of ruminant products. Applied Sciences,13(20):11605, https://doi.org/10.3390/app132011605.
• Canbolat, Ö. (2012). Bazı esansiyel yağlarin in vitro sindirim, rumen fermantasyonu ve metan gazi üretimi üzerine etkileri. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2(1):91-98.
• Canbolat, Ö., Kalkan, H., Karaman, Ş. ve Filya, İ. (2011). Esansiyel yağların sindirim, rumen fermantasyonu ve mikrobiyal protein üretimi üzerine etkileri. Kafkas Ünviveristesi Veterinerlik Fakültesi Dergisi, 17 (4): 557-565.
• Canbolat, Ö., Karaman, Ş. ve Filya, İ. (2010). Farklı kekik yağı dozlarının mısır silajının sindirimi ve rumen fermentasyonu üzerine etkileri. Kafkas Ünviveristesi Veterinerlik Fakültesi Dergisi, 16 (6): 933-939.
• Castillejos, L., Calsamigla, S., Ferret, A. and Losa, R. (2007). Effects of does and adaptation time of a specific blend of essential oil compounds on rumen fermentation. Animal Feed Science and Technology, 132: 186-201.
• Chaves, A.V., He, M.L., Yang, W.Z., Hristov, A.N., McAllister, T.A. and Benchaar, C. (2008). Effect of Essential Oils on Proteolytic, Deminative and Methanogenic Activites of Mixed Ruminal Bacteria. Canadian Journal of Animal Science, 88: 117-122.
• Duncan, D. B. (1955). Multiple range and multiple f tests. Biometrics, 11: 1-42.
• Fouts, J.Q., Honan, M.C., Roque, B.M., Tricarico, J.M. and Kebreab, E. (2022). Enteric methane mitigation interventions. Translational Animal Science, 6(2):1-16.
• Goel, G., Makkar, H. P. S., & Becker, K. (2008). Effects of Sesbania sesban and Carduus pycnocephalus leaves and their extracts on partitioning of nutrients from roughage-and concentrate-based feeds to methane. Animal Feed Science and Technology, 147(1-3), 72-89.
• Gül, H., Avcı, M. ve Kaplan, O. (2017). Bazı kaba yemlere çörek otu, kekik otu ve yağlari ilavesinin in vitro organik madde sindirimi ve metan üretimi üzerine etkileri. Harran Üniversitesi Veterinerlik Fakültesi Dergisi, 6(2): 167-173.
• Kaya, A., Kaya, H. ve Çelebi, Ş. (2012). ruminant hayvanlarda metan üretimini azaltmaya yönelik çalışmalar. Atatürk Üniversitesi Ziraat Fak. Derg., 43(2): 197-204.
• Kaya, A. ve Kaya, A. (2021). The effect of some vegetable oils added to dairy calf rations on in vitro feed value and enteric methane production. Journal of Agricultural Production, 2: 1-6.
• Kaya, A., Başer, A., Kaya, A., Selçuk, B., Cengiz, T., 2022. Determination of the antimethanogenic properties of sumac leaves (rhus coriaria l.) subsitution at different ratios instead of corn silage in sheep rations by in vitro gas production method. Turkish Journal of Agriculture - Food Science and Technology, 10(2): 309–312.
• Kaya, E. (2022). Assessing the effects of clove oil on gas and methane production of some roughages using in vitro gas production. Journal of Agriculture, 5(2): 14-20.
• McIntosh, F.M., Williams, P., Losa, R., Wallace, R.J., Beever, D.A. and Newbold, C.J. (2003). Effects of essential oil on rumenial microorganism and their protein metabolism. Applied and Environmental Microbiology, 69(8): 5011-5014.
• Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W. (1979). The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, 93, 217-222.
• Menke, K.H. and Steingass, H. (1988). Estimation of energetic feed value obtained from chemical analysis and in vitro gas production. Animal Research Development, 28: 7-55.
• Monteiro,V.C., Miles, N.L., Richardson, S.J., Barkley, Z. Haupt, B.J., Lyon, D., Hmiel, B. and Davis, K.J. (2022). Methane, carbon dioxide, hydrogen sulfide and isotopic rations of methane observations from the Permian Basin tower network. Earth System Science Data (ESSD), 14: 2401-2417.
• Öztürk, H. (2007). Küresel ısınmada ruminantların rolü. Veteriner Hekimler Derneği Dergisi, 78(1): 17-21.
• Patra, A. K. and Yu, Z. (2012). Effect of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations. Applied and Environmental Microbiology, 78 (12): 4271-4280.
• Rofig, M.N., Negara, W., Martono, S., Gopar, R.A. and Boga, M. (2021). Potential Effect of Some Essential Oils on Rumen Methane Reduction and Digestibility by In Vitro Incubation Technique. The 8th International Conference on Sustainable Agriculture and Environment, 905(2021) 012138.
• Rofiq, M.N., Görgülü, M. ve Boğa, M. (2012). Karanfil Uçucu Yağinin (Clove Oil) Ruminantlarda In Vitro Gerçek KM ve NDF Sindirilebilirliği ve Yemin Enerji İçeriğine Etkileri, 8. Ulusal Zootekni Öğrenci Kongresi, P.127-129, 7-19 Mayıs 2012, Şanlıurfa, Turkiye.
• Roy, D., Tomar, S. and Kumar, V. (2015). Rumen modulatory effect of thyme, clove and peppermint oils in vitro using buffalo rumen liquor. Veterinary World, 8: 203.
• Sandner, G., Heckmann, M. and Weghuber, J. (2020). Immunomodulatory activities of selected essential oils. Biomolecules,10: 1139.
• Şahan, Z., Tel, A.Z. and Kutay, H. (2021). Effect of cyclotrichium niveum essential oil on rumen microbial fermentation and in vitro digestibility of barley. Tekirdağ Ziraat Fakültesi Dergisi, 18 (1):138-145.
• Şehitoğlu M. and Kaya, H., (2021). The Effect of Clove Oil Supplementation in Laying Hen Diets on Performance, Egg Quality, Some Blood Parameters, and Yolk TBARS. Turkish Journal of Agriculture - Food Science and Technology, 9(12): 2213-2218.
• SPSS. Windows User’s Guide. Version 20.0, SPSS Inc., Michigan Ave., Illinois, USA., Chicago
• Temiz, U. and Ozturk, E. (2020). Antioxidant and Immunostimulant Effects of Some Medicinal and Aromatic Plants. In Proceedings of the4th International Eurasian Agriculture and Natural Sciences Congress, Online, 30–31 October 2020; pp. 312–317. Available online:https://online.agrieurasia.com/TR/ (accessed on 22 October 2023).
• Torres, R.N.S., Moura, D.C., Ghedini, C.P., Ezequiel, J.M.B. ve Almeida, M.T.C. (2020). Meta-analysis of the effects of essential oils onruminal fermentation and performance of sheep.(2020). Small Ruminant Research, 189, 106148.
• Ünal, K.ve Karakaya, M. (2017). Farklı Hayvansal Yağlar İlave Edilerek Üretilen Sucukların Bazı Fizikokimyasal Özellikleri Üzerine Karanfil ve Tarçının Etkisi. Tekirdağ Ziraat Fakültesi Dergisi: 14 (03): 55-65.
• Van Soest, P.J., Robertson, J. D., and Lewis, B. A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74: 3583-3597.