SHSY5Y NÖROBLASTOMA HÜCRELERİNDE SKOPOLETİN NÖROTOKSİSİTESİNE BAĞLI EMPEDANS DEĞİŞİKLİKLERİNİN GERÇEK ZAMANLI ANALİZİ

Öz

Bitki kumarinleri, insan diyetinin önemli bileşenleridir ve birçoğunun kansere karşı koruyucu ve tedavi edici özelliklere sahip olduğu bilinmektedir. Doğal bir kumarin bileşeni olan skopoletinin birçok kanser hücresinin büyümesini engellediği gösterilmiştir. Bu çalışmada, insan nöroblastom hücreleri SHSY5Y'ye karşı skopoletinin sitotoksik aktivitesi incelendi. Scopoletin aktivitesi, Sulphorhodamine B (SRB) testi ve xCELLigence sistemi ile değerlendirildi. Skopoletinin insan SHSY5Y nöroblastom hücre canlılığını azalttığı gösterildi. Scopoletin hücre büyümesini inhibe etti ve IC50 değerleri 48 ve 72 saat için sırasıyla 91,82 ve 79,19 µM’dir. Bu çalışmadan elde edilen bulgular, skopoletinin SHSY5Y hücreleri üzerindeki büyüme önleyici ve sitotoksik etkilerinin, nöroblastom kanseri için yeni tedavi rejimlerinin geliştirilmesinde önemli olabileceğini kanıtladı.

Anahtar Kelimeler

• Sitotoksisite
• skopoletin
• SH-SY5Y
• SRB
• xCELLigence

REAL TIME ANALYSIS OF IMPEDANCE ALTERATIONS BY THE NEUROTOXICITY OF SCOPOLETIN ON SHSY5Y NEUROBLASTOMA CELLS

Öz

Plant coumarins are important components of the human diet and a number of them are considered to possess chemopreventive and therapeutic properties against cancer. Scopoletin, a natural coumarin component has been shown to inhibit the growth of many cancer cells. In this study, the cytotoxic activity of scopoletin was studied against human neuroblastoma cells SHSY5Y. The activity of scopoletin was evaluated by Sulphorhodamine B (SRB) assay and xCELLigence system. It was demonstrated that scopoletin reduced human SHSY5Y neuroblastoma cell viability. Scopoletin inhibited the growth of the cell line and the IC50 values were 91.82 and 79.19 μM for 48 h and 72 h, respectively. The findings from this study demonstrated that the growth inhibitory and cytotoxic effects of scopoletin on SHSY5Y cells may prove essential in the development of novel treatment regimens for neuroblastoma cancer.

Anahtar Kelimeler

• Cytotoxicity
• scopoletin
• SHSY5Y
• SRB
• xCELLigence

Kaynakça

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