Relationship Between B-Type Natriuretic Peptide Levels With Some Blood Parameters in Diarrheic Calves

Yıl 2025, Cilt: 34 Sayı: 3, 374 - 381, 30.12.2025

İlknur Karaca Bekdik , Emre Tüfekçi , Mehmet Akif Rıhtım , Aytaç Akçay , Öznur Aslan

https://doi.org/10.34108/eujhs.1659353

Öz

This study aimed to determine the relationship of plasma B-type natriuretic peptide levels with hematological and some serum biochemical parameters (serum creatinine, sodium, potassium, and total protein levels) in healthy and neonatal diarrhea calves. The material of the study was consisted of newborn calves with 44 diarrheic and 40 healthy, which were brought to Erciyes University Veterinary Faculty Training, Research and Application Hospital Internal Medicine Clinics for diagnosis, treatment and control purposes. Blood and serum samples were taken from the calves included in the study. Hematological parameters, plasma B-type natriuretic peptide, serum creatinine, sodium, potassium and total protein levels were determined from the samples taken. There was no statistically significant difference in plasma B-type natriuretic peptide levels between diarrheic and healthy groups (p>0.05). However, the Cohen’s d effect size to determine clinical significance was calculated as 0.228. This result indicated that an average of 53 units (1259-1206) lower plasma B-type natriuretic peptide value in the diarrheal group could be considered clinically significant. In addition, a statistically significant difference was found between the diarrheal and healthy groups in creatinine, Na, to tally leukocytes, neutrophils and mean platelet volume parameters (p<0.05). The only effective variable on plasma B-type natriuretic peptide was determined as creatine. As a result, it was determined that the plasma B-type natriuretic peptide level in calves with diarrhea was lower than the healthy ones and there was a correlation between the plasma B-type natriuretic peptide levels and the serum creatinine levels.

Anahtar Kelimeler

Biochemistry , B-type natriuretic peptide , calf , calf diarrhea , hematology

Etik Beyan

Ethics committee approval was received for this study from the Animal Experiments Ethics Committee of Erciyes University (Date: 07.04.2021, Number: 21/96).

İshalli Buzağılarda B-Tipi Natriüretik Peptit Seviyeleri ve Bazı Kan Parametreleri Arasındaki İlişki*

Öz

Bu çalışmanın amacı neonatal ishalli ve sağlıklı buzağılarda plazma B-tipi natriüretik peptit seviyeleri ile hematolojik ve bazı serum biyokimyasal parametreleri (serum kreatinin, sodyum, potasyum ve total protein seviyeleri) arasındaki ilişkinin belirlenmesidir. Çalışma materyalini Erciyes Üniversitesi Veteriner Fakültesi Eğitim Araştırma Uygulama Hastanesi İç Hastalıkları Anabilim Dalı Kliniğine teşhis, tedavi ve kontrol amacı ile getirilen 44 adet ishalli ve 40 adet sağlıklı yeni doğan buzağı oluşturdu. Çalışmaya dâhil edilen buzağılardan alınan tam kan ve serum örneklerinde hematolojik parametreler ile biyokimyasal parametreler belirlendi. İshalli ve sağlıklı gruplar arasında plazma B-tipi natriüretik peptit düzeyi bakımından istatistiksel olarak anlamlı farklılık bulunmadı (p>0.05). Ancak klinik anlamlılığın tespiti amacıyla Cohen d etki büyüklüğü 0.228 olarak hesaplandı. Bu durum ishalli grupta plazma B-tipi natriüretik peptit değerinin ortalama 53 birim (1259-1206) az olmasının klinik olarak anlamlı kabul edilebileceğini gösterdi. Ayrıca çalışmada ishalli ve sağlıklı gruplar arasında kreatinin, Na, lökosit, nötrofil ve ortalama platelet hacmi değişkenleri bakımından istatistiksel olarak anlamlı farklılık bulundu (p<0.05). Plazma B-tipi natriüretik peptit üzerine tek etkili değişken kreatinin olarak belirlendi. Sonuç olarak, ishalli buzağılarda plazma B-tipi natriüretik peptit seviyesinin sağlıklılara göre düşük olduğu ve plazma B-tipi natriüretik peptit düzeyi ile serum kreatinin seviyesi arasında negatif korelasyon olduğu belirlendi.

Anahtar Kelimeler

Biyokimya , B-tip natriüretik peptid , buzağı , buzağı ishalleri , hematoloji

Etik Beyan

Bu çalışma için Erciyes Üniversitesi Hayvan Deneyleri Yerel Etik Kurulundan (Tarih: 07.04.2021, Sayı: 21/96) onay alınmıştır.

Kaynakça

• McClure JT. Oral fluid therapy for treatment of neonatal diarrhoea in calves. Vet J. 2001;162(2):87-89. doi:10.1053/tvjl.2001.0597.
• Ekinci G, Tüfekçi E, Onmaz AC, Çitil M, Keleş İ, Güneş V. Erciyes Üniversitesi Hayvan Hastanesi’ne 2019-2021 Yılları Arasında Getirilen Neonatal İshalli Buzağılarda Majör Enteropatojenlerin Yaygınlığının Araştırılması. Erciyes Üniv Vet Fak Derg. 2022;19(2):113-122. doi:10.32707/ercivet.1142579
• Keleş İ, Ekinci G, Tüfekçi E, et al. Etiological and predisposing factors in calves with Neonatal Diarrhea: a clinical study in 270 caseseries. Kafkas Univ Vet Fak Derg. 2022;28(3):315-326. doi:10.9775/kvfd.2021.26981.
• Smith GW. Treatment of calf diarrhea: oral fluid therapy. Vet Clin North Am Food Anim Pract. 2009;25(1):55-72. doi:10.1016/j.cvfa.2008.10.006.
• Gomez DE, Lofstedt J, Stämpfli HR, Wichtel M, Muirhead T, McClure JT. Contribution of unmeasured anions to acid–base disorders and its association with altered demeanor in 264 calves with neonatal diarrhea. J Vet Intern Med. 2013;27(6):1604-1612. doi:10.1111/jvim.12193.
• Uçar F, Turhan S. Natriüretik peptidler. Türk Hij Den Biyo lDerg. 2005;62(1):49-54.
• Demirtaş S, Karaboğa İ, Karaca T. Natriüretik peptitler. Int J Basic Clin Med. 2014;2(3):157-164.
• Levin ER, Gardner DG, Samson WK. Natriureticpeptides. N Engl J Med. 1998;339(5):321-328. doi:10.1056/NEJM199807303390507.
• Potter LR, Abbey-Hosch S, Dickey DM. Natriuretic peptides, their receptors, and cyclic guanosine monophosphate-dependent signaling functions. Endocr Rev. 2006;27(1):47-72. doi:10.1210/er.2005-0014.
• Goggs R. Pulmonary Thromboembolism. Bruyette D, ed. USA: Wiley-Blackwell; 2020:318.
• McCullough PA, Sandberg KR. B-type natriuretic peptide and renal disease. Heart Fail Rev. 2003;8(4):355-358. doi:10.1023/a:1026195332025.
• deBold AJ, Borenstein HB, Veress AT, Sonnenberg H. A rapid and potent natriuretic responsetoin travenous injection of atrial myocardial extracts in rats. Life Sci. 1981;28(1):89-94. doi:10.1016/0024-3205(81)90370-2.
• Vanderheyden M, Bartunek J, Goethals M. Brain and other natriuretic peptides: molecular aspects. Eur J Heart Fail. 2004;6(3): 261-268.doi: 10.1016/j.ejheart.2004.01.004.
• Sudoh T, Kangawa K, Minamino N, Matsuo H. A new natriuretic peptide in porcine brain. Nature. 1988;332(6159):78-81. doi:10.1038/332078a0.
• Hall C. Essential biochemistry and physiology of (NT‐pro) BNP. Eur J Heart Fail. 2004;6(3): 257-260. doi: 10.1016/j.ejheart.2003.12.015.
• Azzazy HME, Christenson RH. B-type natriuretic peptide: physiologic role and assay characteristics. Heart Fail Rev. 2003;8(4):315-320. doi:10.1023/a:1026182912461.
• Ozgo M. Renin-angiotensin-aldosterone system versus osmotic pressure of blood plasma in calves in the neonatal period. Electron J Pol ArgicUniv Ser Vet Med. 2001;4:2.
• Ozgo M. The relationship between blood plasma AVP and electrolytes concentration in calves in the neonatal period (in Polish). Folia Univ Agric Stetin. 2000;39:119-124.
• Dratwa A. Atrial natriuretic peptide and renal haemodynamics in new born calves. Acta Vet Brno. 2006; 75:477-483. doi:10.2754/avb200675040477.
• Volpe M. Natriuretic peptides and cardio-renal disease. Int J Cardiol. 2014;176(3):630-639.doi: 10.1016/j.ijcard.2014.08.032.
• Okamoto R, Ali Y, Hashizume R, Suzuki N, Ito M. BNP as a major player in the heart-kidney connection. Int J Mol Sci. 2019;20(14):3581. doi:10.3390/ijms20143581.
• Cohen J. Statistical Power Analysis for the Behavioral Sciences. New York: Lawrence Erlbaum Associates; 1988:274-288.
• Taylor JA. Leukocyte Responsees in Ruminants. In: Bernart FF, Joseph GZ, Nemi CJ, eds. Schalm’s Veterinary Hematology. Philadelphia, PA: Lippincott Wiliams and Wilkins; 2000:391-401.
• Ekinci G, Tüfekçi E, Cissé Y, et al. Chloride and lactate as prognostic indicators of calf diarrhea from eighty-nine cases. J Vet Sci. 2024;25(3): e38. doi:10.4142/jvs.23155.
• Ünal CN, Uslu P. Evaluation of Hematology and blood gasparameters in calves with sepsis. FU Vet J Health Sci. 2024;38(2):157-163.
• Uztimür, M. Diagnostic significance of mean platelet volume and erythrocyte distribution width in calves with sepsis. Erciyes Üniv Vet Fak Derg. 2024;21(3):194-201.
• Mete E, Akelma AZ, Cizmeci MN, et al. Decreased mean platelet volume in children with acute rotavirus gastroenteritis. Platelets. 2013;25(1):51-54.
• Tanju C, Ekrem G, Emel AB, Nur A. (2014). Mean platelet volume as a negative marker of inflammation in children with rotavirus gastroenteritis. Iran J Pediatr. 2014;24(5):617-622.
• Gasparyan AY, Ayvazyan L, Mikhailidis DP, et al. Mean platelet volume: A link between thrombosis and inflammation? Curr Pharm Des. 2011;17(1):47-58.
• Gasparyan AY, Sandoo A, Stavropoulos-Kalinoglou A, et al. Mean platelet volume in patients with rheumatoid arthritis: The effect of anti-TNF-alpha therapy. Rheumatol Int. 2010;30(8):1125-1129.
• Makdam M, Basbugan Y. Relationship between Cystatin C with some hematological and biochemical parameters in neonatal calf diarrhea. TJVR. 2020;4(2):79-87.
• Song RH, Kang JH, Park KM, Youm JH, Park JH. Analysis of hematological changes in normal and diarrhea calves. Korean J Vet Serv. 2020;43(3):161-165. doi:10.7853/kjvs.2020.43.3.161
• Atcalı T, Yıldız R. Neonatal Buzağı İshallerinde Farklı Etiyolojik Faktörlerin Hemogram Parametreleri Üzerine Etkisi. MAKÜ Sag Bil Enst Derg. 2020;8(3):119-127. doi:10.24998/maeusabed.823819.
• Dratwa A, Skrzypczak WF, Ozgo M. Atrial natriuretic peptide and volemia regulation in new born calves. Electron J Pol Agric Univ Ser Anim Husb. 2004; 7:2.
• Dratwa-Chałupnik A, Herosimczyk A, Lepczyński A, Skrzypczak WF. Calves with diarrhea and a water-electrolyte balance. Med Weter. 2012;68(1):5-8.
• Bradshaw C, Zheng Y, Silver SA, Chertow GM, Long J, Anand S. Acute kidney injury due to diarrhea lillness requiring hospitalization: Data from the National Inpatient Sample. J Gen Intern Med. 2018;33(9):1520-1527. doi:10.1007/s11606-018-4531-6.
• Gowda S, Desai PB, Kulkarni SS, Hull VV, Math AAK, Vernekar SN. 2010. Markers of renal functiontests. North Am J Med Sci. 2010;2:170-173.
• Başer DF, Civelek T. Akut İshalli Neonatal Buzağılarda Venöz Asit-Baz Durumu ve Renal Fonksiyon Arası Korelasyon. Kocatepe Vet J. 2013;6(1):25-31. doi:10.5578/kvj.5516.
• Singh M, Gupta VK, Mondal DB, et al. A study on alteration in Haemato-biochemical parameters in Colibacillosis affected calves. Int J Adv Res. 2014;2(7):746-750.
• Seifi HA, Mohri M, Shoorei E, Farzaneh N. Using haematological and serum biochemical findings as prognostic indicators in calf diarrhoea. Comp Clin Path. 2006;15(3):143-147. doi:10.1007/s00580-006-0620-8.
• Savoj J, Becerra B, Kim JK, et al. Utility of cardiac biomarkers in the setting of kidney disease. Nephron. 2019;141(4):227-235. doi:10.1159/000495946.
• Guzelbektes H, Coskun A, Sen I. Relationship between the degree of dehydration and the balance of acid-based changes in dehydrated calves with diarrhoea. Bull Vet Inst Pulawy. 2007;51(1):83-87.